Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Alkalilimnicola; Alkalilimnicola ehrlichii

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2862 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0AAP8|Q0AAP8_ALKEH Uncharacterized protein OS=Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1) OX=187272 GN=Mlg_0735 PE=4 SV=1
MM1 pKa = 7.95KK2 pKa = 10.31FRR4 pKa = 11.84LLLALAALYY13 pKa = 7.22PASMTAVAQTDD24 pKa = 3.68TATFDD29 pKa = 3.18VTATVDD35 pKa = 3.5PTCTVDD41 pKa = 4.09ADD43 pKa = 3.67NLVFGTYY50 pKa = 10.49DD51 pKa = 3.62PFSDD55 pKa = 3.86TPLDD59 pKa = 3.79EE60 pKa = 4.44NSEE63 pKa = 3.84IRR65 pKa = 11.84VQCTSDD71 pKa = 2.97TPYY74 pKa = 11.06DD75 pKa = 3.39IGLDD79 pKa = 3.8DD80 pKa = 5.12GDD82 pKa = 3.69NTGAEE87 pKa = 4.61GEE89 pKa = 4.18RR90 pKa = 11.84RR91 pKa = 11.84MALADD96 pKa = 3.45EE97 pKa = 4.68SDD99 pKa = 3.46FLEE102 pKa = 4.25YY103 pKa = 10.81DD104 pKa = 4.15LYY106 pKa = 11.16HH107 pKa = 7.3DD108 pKa = 3.99NHH110 pKa = 6.98GGTSWGDD117 pKa = 2.93IDD119 pKa = 5.73SGAEE123 pKa = 3.82LTGLSGTGSEE133 pKa = 4.13QSYY136 pKa = 9.18VVYY139 pKa = 10.69GRR141 pKa = 11.84IFAEE145 pKa = 3.85QSVAVGNYY153 pKa = 8.58VDD155 pKa = 4.21TIEE158 pKa = 4.84VTVKK162 pKa = 8.76WW163 pKa = 3.67

Molecular weight: 17.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0AA45|Q0AA45_ALKEH Cation diffusion facilitator family transporter OS=Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1) OX=187272 GN=Mlg_0938 PE=4 SV=1
MM1 pKa = 6.96KK2 pKa = 9.5TAIPVNTQGRR12 pKa = 11.84VSGHH16 pKa = 5.49FAKK19 pKa = 10.57APYY22 pKa = 9.41MLVLTDD28 pKa = 3.38EE29 pKa = 4.95GQRR32 pKa = 11.84QWVANPMDD40 pKa = 4.82ADD42 pKa = 4.12RR43 pKa = 11.84CSGRR47 pKa = 11.84CKK49 pKa = 10.49LLAEE53 pKa = 4.69LEE55 pKa = 4.41QAGVTRR61 pKa = 11.84VLVRR65 pKa = 11.84QIGQRR70 pKa = 11.84TLGRR74 pKa = 11.84FLRR77 pKa = 11.84AGLQVYY83 pKa = 9.25RR84 pKa = 11.84LPAGATALPRR94 pKa = 11.84AAAIPAEE101 pKa = 4.2AQALSQASQGRR112 pKa = 11.84PSKK115 pKa = 10.37PRR117 pKa = 11.84ASHH120 pKa = 6.49GEE122 pKa = 4.02GTAGMTGCCGHH133 pKa = 6.85HH134 pKa = 6.35

Molecular weight: 14.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2862

0

2862

983950

30

6062

343.8

37.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.535 ± 0.055

0.928 ± 0.018

5.951 ± 0.049

6.752 ± 0.047

3.149 ± 0.029

8.783 ± 0.053

2.547 ± 0.024

4.013 ± 0.033

2.108 ± 0.036

11.327 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.2 ± 0.026

2.258 ± 0.023

5.504 ± 0.036

3.792 ± 0.03

8.681 ± 0.059

4.415 ± 0.024

4.731 ± 0.029

7.465 ± 0.042

1.53 ± 0.023

2.331 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski