Lachnoclostridium sp. An169

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnoclostridium; unclassified Lachnoclostridium

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4280 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4NJX2|A0A1Y4NJX2_9FIRM Cytidylate kinase OS=Lachnoclostridium sp. An169 OX=1965569 GN=B5F07_18555 PE=4 SV=1
MM1 pKa = 8.67KK2 pKa = 9.83MMKK5 pKa = 9.95KK6 pKa = 9.87AASVALASALVVSLAACGSNGGDD29 pKa = 3.48GNSNSGSTGSDD40 pKa = 2.28TFMIGGIGPTTGDD53 pKa = 3.02NAIYY57 pKa = 8.55GTAVKK62 pKa = 10.63NGIQLAVDD70 pKa = 4.95EE71 pKa = 4.7INEE74 pKa = 4.12AGGINGYY81 pKa = 9.16QISYY85 pKa = 10.25KK86 pKa = 10.59FEE88 pKa = 5.67DD89 pKa = 4.18DD90 pKa = 3.5QSDD93 pKa = 3.86SEE95 pKa = 4.59KK96 pKa = 11.03SVNAYY101 pKa = 7.69NTLKK105 pKa = 10.75DD106 pKa = 2.94WGMQMLVGTVTSTPCVAVVEE126 pKa = 4.56EE127 pKa = 4.2THH129 pKa = 6.17VDD131 pKa = 2.97NMFQFTPSATSVEE144 pKa = 4.32SVQYY148 pKa = 11.31DD149 pKa = 3.34NAFRR153 pKa = 11.84MCFSDD158 pKa = 4.25PSQGTVSADD167 pKa = 3.43YY168 pKa = 10.88IADD171 pKa = 3.56NGLATKK177 pKa = 10.3IGVIYY182 pKa = 10.15DD183 pKa = 3.45SSSTYY188 pKa = 9.52STGIYY193 pKa = 10.11QNFAAEE199 pKa = 4.23ADD201 pKa = 4.07VKK203 pKa = 11.06GLEE206 pKa = 4.22IVSAEE211 pKa = 4.04AFTSDD216 pKa = 3.78SNSDD220 pKa = 3.8FNVQLQKK227 pKa = 11.28AKK229 pKa = 10.65DD230 pKa = 3.4AGAEE234 pKa = 4.19LVFLPIYY241 pKa = 9.08YY242 pKa = 9.88QEE244 pKa = 4.49ASLILAQADD253 pKa = 3.63RR254 pKa = 11.84MGYY257 pKa = 8.06APKK260 pKa = 10.11WFGVDD265 pKa = 3.43GMDD268 pKa = 5.99GILNLDD274 pKa = 3.83GFDD277 pKa = 3.55ASLAEE282 pKa = 4.02GVMFLTPFTPTADD295 pKa = 4.21DD296 pKa = 3.84EE297 pKa = 4.45ATQTFVANYY306 pKa = 7.96EE307 pKa = 4.2AEE309 pKa = 4.4FGDD312 pKa = 4.31TPIQFAADD320 pKa = 4.19AYY322 pKa = 10.7DD323 pKa = 3.49CLYY326 pKa = 10.42VIKK329 pKa = 10.49AAAEE333 pKa = 4.13KK334 pKa = 10.86AGITPDD340 pKa = 3.31MSVSDD345 pKa = 3.74ICDD348 pKa = 3.25AMKK351 pKa = 9.59TAMTEE356 pKa = 3.69ITVDD360 pKa = 3.43GLTGKK365 pKa = 10.38QITWGEE371 pKa = 3.93DD372 pKa = 3.37GEE374 pKa = 4.37PSKK377 pKa = 11.25EE378 pKa = 3.88PTVVVIHH385 pKa = 7.0DD386 pKa = 3.74GAYY389 pKa = 9.18TVMM392 pKa = 4.95

Molecular weight: 41.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4NMH4|A0A1Y4NMH4_9FIRM Secretion protein F OS=Lachnoclostridium sp. An169 OX=1965569 GN=B5F07_15575 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.18QLSAA44 pKa = 3.9

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4280

0

4280

1444677

27

2981

337.5

37.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.669 ± 0.037

1.542 ± 0.016

5.71 ± 0.033

8.02 ± 0.042

4.138 ± 0.029

7.456 ± 0.032

1.735 ± 0.014

6.727 ± 0.037

5.863 ± 0.033

8.902 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.065 ± 0.018

3.947 ± 0.026

3.555 ± 0.02

3.134 ± 0.022

5.332 ± 0.036

5.724 ± 0.03

5.369 ± 0.033

6.789 ± 0.031

1.07 ± 0.014

4.252 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski