Salmonella phage SEN4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Senquatrovirus; Salmonella virus SEN4

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0M4R507|A0A0M4R507_9CAUD Late control D protein OS=Salmonella phage SEN4 OX=1647465 GN=SEN4_28 PE=4 SV=1
MM1 pKa = 7.64SGTIDD6 pKa = 4.84LSQLPPPVVVEE17 pKa = 4.06PLDD20 pKa = 3.89FEE22 pKa = 4.7TLFAQRR28 pKa = 11.84KK29 pKa = 6.95AAFIAMYY36 pKa = 10.23PEE38 pKa = 4.92DD39 pKa = 4.31EE40 pKa = 4.28QEE42 pKa = 4.23EE43 pKa = 4.27IARR46 pKa = 11.84TLEE49 pKa = 4.3LEE51 pKa = 4.2SEE53 pKa = 4.87PITMLLEE60 pKa = 4.14EE61 pKa = 4.03NCYY64 pKa = 10.44RR65 pKa = 11.84EE66 pKa = 4.01LLLRR70 pKa = 11.84QRR72 pKa = 11.84VNEE75 pKa = 3.92AARR78 pKa = 11.84AVMLAYY84 pKa = 9.8STDD87 pKa = 3.45SDD89 pKa = 4.56LDD91 pKa = 3.66NLAVNFNVEE100 pKa = 3.66RR101 pKa = 11.84LTIQEE106 pKa = 4.41EE107 pKa = 4.42DD108 pKa = 4.01DD109 pKa = 4.2SVTPPIEE116 pKa = 4.01AVVEE120 pKa = 4.06SDD122 pKa = 2.72ADD124 pKa = 3.56LRR126 pKa = 11.84TRR128 pKa = 11.84TQQAFEE134 pKa = 4.23GLSVAGPTAAYY145 pKa = 8.17EE146 pKa = 4.04FWGRR150 pKa = 11.84SADD153 pKa = 3.49GRR155 pKa = 11.84VADD158 pKa = 4.7ISAVSPTPACVTISVLSRR176 pKa = 11.84EE177 pKa = 4.37GDD179 pKa = 3.47GTASDD184 pKa = 4.66DD185 pKa = 3.98LLSVVAAALNDD196 pKa = 3.71EE197 pKa = 4.58EE198 pKa = 4.51VRR200 pKa = 11.84PVADD204 pKa = 3.01RR205 pKa = 11.84VTVQSADD212 pKa = 3.06IVPYY216 pKa = 10.65QIDD219 pKa = 3.74ATLYY223 pKa = 9.62IYY225 pKa = 9.19PGPEE229 pKa = 3.79AEE231 pKa = 4.54PVRR234 pKa = 11.84QASEE238 pKa = 4.05QQLQAYY244 pKa = 6.87ITAQNRR250 pKa = 11.84LGRR253 pKa = 11.84DD254 pKa = 2.99IRR256 pKa = 11.84ISAIYY261 pKa = 9.65AALHH265 pKa = 5.3VEE267 pKa = 4.81GVQRR271 pKa = 11.84VEE273 pKa = 3.98LAQPVADD280 pKa = 4.37IVLSDD285 pKa = 3.81YY286 pKa = 10.63QASHH290 pKa = 5.6CTEE293 pKa = 3.71YY294 pKa = 10.62TITVGGYY301 pKa = 10.39DD302 pKa = 3.42EE303 pKa = 4.8

Molecular weight:
33.23 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0M5M1H9|A0A0M5M1H9_9CAUD Integrase OS=Salmonella phage SEN4 OX=1647465 GN=SEN4_31 PE=3 SV=1
MM1 pKa = 7.74TGQQTDD7 pKa = 3.79PLFQQLDD14 pKa = 3.38DD15 pKa = 3.49WLARR19 pKa = 11.84VAAQLSPGHH28 pKa = 5.97RR29 pKa = 11.84RR30 pKa = 11.84KK31 pKa = 8.87LTRR34 pKa = 11.84DD35 pKa = 3.19VAIGLRR41 pKa = 11.84KK42 pKa = 9.46RR43 pKa = 11.84QQKK46 pKa = 10.28RR47 pKa = 11.84IASQKK52 pKa = 8.7NPSGEE57 pKa = 4.15SYY59 pKa = 9.16QARR62 pKa = 11.84RR63 pKa = 11.84RR64 pKa = 11.84KK65 pKa = 9.45ILRR68 pKa = 11.84TQGGIKK74 pKa = 9.63FIWNDD79 pKa = 3.07EE80 pKa = 3.84ARR82 pKa = 11.84EE83 pKa = 3.95LRR85 pKa = 11.84NWRR88 pKa = 11.84TTGRR92 pKa = 11.84GEE94 pKa = 3.67HH95 pKa = 6.29RR96 pKa = 11.84AITGYY101 pKa = 10.81DD102 pKa = 3.18VDD104 pKa = 4.59RR105 pKa = 11.84GALRR109 pKa = 11.84TFYY112 pKa = 10.87KK113 pKa = 10.27RR114 pKa = 11.84DD115 pKa = 2.95IQRR118 pKa = 11.84YY119 pKa = 8.88IEE121 pKa = 4.19INLNQSKK128 pKa = 9.4QNRR131 pKa = 11.84TRR133 pKa = 11.84KK134 pKa = 10.36DD135 pKa = 3.07PMFRR139 pKa = 11.84KK140 pKa = 9.77LRR142 pKa = 11.84TARR145 pKa = 11.84FLKK148 pKa = 10.65AYY150 pKa = 6.05GTSGIAVVGFQGHH163 pKa = 4.6TAEE166 pKa = 4.51IASVHH171 pKa = 5.79QYY173 pKa = 11.53GEE175 pKa = 3.9VDD177 pKa = 3.46NVVPGARR184 pKa = 11.84ARR186 pKa = 11.84YY187 pKa = 7.83PVRR190 pKa = 11.84EE191 pKa = 4.09LLGMTEE197 pKa = 5.85GDD199 pKa = 4.56LDD201 pKa = 3.8WLADD205 pKa = 3.66TVVAFMQEE213 pKa = 3.51II214 pKa = 3.73

Molecular weight:
24.7 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

10358

38

974

220.4

24.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.34 ± 0.59

1.197 ± 0.191

6.15 ± 0.295

5.793 ± 0.31

3.437 ± 0.318

7.289 ± 0.296

1.757 ± 0.189

5.252 ± 0.209

5.223 ± 0.326

8.37 ± 0.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.365 ± 0.159

3.92 ± 0.199

4.518 ± 0.207

4.364 ± 0.381

6.343 ± 0.507

6.179 ± 0.323

6.246 ± 0.401

6.488 ± 0.226

1.574 ± 0.163

3.196 ± 0.241

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski