Mycetocola zhujimingii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Mycetocola

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2928 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U1TBR1|A0A2U1TBR1_9MICO Uncharacterized protein OS=Mycetocola zhujimingii OX=2079792 GN=DF223_11975 PE=4 SV=1
MM1 pKa = 7.58NIGSRR6 pKa = 11.84ITAAAALSVGIVALTGCTAAEE27 pKa = 3.83NSAALEE33 pKa = 4.35NGDD36 pKa = 4.14EE37 pKa = 4.34KK38 pKa = 11.25DD39 pKa = 3.38VSIAVFNGWDD49 pKa = 3.46EE50 pKa = 4.32AVATSVLWEE59 pKa = 4.94SILADD64 pKa = 3.11KK65 pKa = 11.02GYY67 pKa = 11.02DD68 pKa = 3.44VTLDD72 pKa = 3.67YY73 pKa = 11.46ADD75 pKa = 4.7PAPVFAGLAAGDD87 pKa = 3.79YY88 pKa = 10.44DD89 pKa = 3.7ATLDD93 pKa = 3.22VWLPFTHH100 pKa = 7.38KK101 pKa = 10.63SYY103 pKa = 11.14LDD105 pKa = 3.65EE106 pKa = 5.34YY107 pKa = 11.05GDD109 pKa = 5.32DD110 pKa = 3.77IVEE113 pKa = 4.15LGAWNNEE120 pKa = 3.69GKK122 pKa = 10.08NAIAVNADD130 pKa = 3.22APIDD134 pKa = 3.88SLAEE138 pKa = 3.83LAEE141 pKa = 4.2NADD144 pKa = 3.7LFGNKK149 pKa = 8.34IVGIEE154 pKa = 3.99PGAGLTMATEE164 pKa = 4.17EE165 pKa = 4.4RR166 pKa = 11.84VIPTYY171 pKa = 10.79GLEE174 pKa = 4.0DD175 pKa = 3.57MEE177 pKa = 5.02YY178 pKa = 8.49VTSSTSAMLTEE189 pKa = 4.59LTSATKK195 pKa = 10.34AGEE198 pKa = 4.34NIAVTLWEE206 pKa = 4.04PHH208 pKa = 4.33WAYY211 pKa = 10.89GEE213 pKa = 4.05FALKK217 pKa = 10.52NLEE220 pKa = 4.32DD221 pKa = 3.99PEE223 pKa = 4.66GALGAVEE230 pKa = 4.91TIHH233 pKa = 7.12AYY235 pKa = 10.59ASTDD239 pKa = 3.53FSEE242 pKa = 4.51SHH244 pKa = 6.06PTAAGWLSDD253 pKa = 4.13FEE255 pKa = 4.49MDD257 pKa = 5.48LDD259 pKa = 3.99TLYY262 pKa = 11.37SLEE265 pKa = 4.05KK266 pKa = 10.84VLFVDD271 pKa = 5.0YY272 pKa = 11.21DD273 pKa = 3.97GDD275 pKa = 3.91DD276 pKa = 3.52YY277 pKa = 12.13APIVEE282 pKa = 5.31KK283 pKa = 10.12WIEE286 pKa = 3.97DD287 pKa = 3.16NRR289 pKa = 11.84EE290 pKa = 3.97YY291 pKa = 11.54VDD293 pKa = 4.19GLTSS297 pKa = 3.2

Molecular weight: 31.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U1TAE4|A0A2U1TAE4_9MICO ATPase OS=Mycetocola zhujimingii OX=2079792 GN=DF223_14490 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2928

0

2928

942499

29

1940

321.9

34.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.545 ± 0.057

0.477 ± 0.01

6.15 ± 0.038

5.711 ± 0.041

3.319 ± 0.027

8.732 ± 0.04

1.867 ± 0.021

4.971 ± 0.036

2.334 ± 0.037

10.13 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.794 ± 0.015

2.363 ± 0.025

5.252 ± 0.031

2.796 ± 0.022

6.696 ± 0.041

6.268 ± 0.036

6.308 ± 0.033

8.788 ± 0.039

1.447 ± 0.022

2.055 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski