Achromobacter phage vB_AxyP_19-32_Axy09

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Krylovirinae; unclassified Krylovirinae

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514CTU5|A0A514CTU5_9CAUD Uncharacterized protein OS=Achromobacter phage vB_AxyP_19-32_Axy09 OX=2591040 GN=Axy09_006 PE=4 SV=1
MM1 pKa = 7.84AEE3 pKa = 4.01DD4 pKa = 3.46SGAIIHH10 pKa = 6.08KK11 pKa = 6.64TTFEE15 pKa = 4.07NAQKK19 pKa = 10.13LAKK22 pKa = 9.89AARR25 pKa = 11.84LTEE28 pKa = 3.7QRR30 pKa = 11.84LRR32 pKa = 11.84YY33 pKa = 9.08YY34 pKa = 11.07ADD36 pKa = 3.5GQAQPGGNRR45 pKa = 11.84GPYY48 pKa = 9.69RR49 pKa = 11.84PLEE52 pKa = 3.95VDD54 pKa = 3.47AAVTDD59 pKa = 4.39LLAAAEE65 pKa = 4.2ACGYY69 pKa = 9.38PVGAGSPVTDD79 pKa = 3.95GQTVPVVGDD88 pKa = 3.75PQQLAQLAVDD98 pKa = 3.72SGGQVTSVTLIPNPDD113 pKa = 3.74YY114 pKa = 11.33DD115 pKa = 4.03PEE117 pKa = 4.33GG118 pKa = 3.67

Molecular weight: 12.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514CTU3|A0A514CTU3_9CAUD Uncharacterized protein OS=Achromobacter phage vB_AxyP_19-32_Axy09 OX=2591040 GN=Axy09_005 PE=4 SV=1
MM1 pKa = 7.29KK2 pKa = 8.81QTPIASAIQPGRR14 pKa = 11.84TSGAQALSALKK25 pKa = 10.0QRR27 pKa = 11.84QLRR30 pKa = 11.84EE31 pKa = 3.5TGMTHH36 pKa = 5.06WQLLEE41 pKa = 4.12LYY43 pKa = 9.04RR44 pKa = 11.84TMLAHH49 pKa = 7.17KK50 pKa = 10.33AKK52 pKa = 10.57GQDD55 pKa = 3.2RR56 pKa = 11.84VWAVFFDD63 pKa = 3.73VTQFPGRR70 pKa = 11.84PTRR73 pKa = 11.84VAGILTNWLRR83 pKa = 11.84CSVLVRR89 pKa = 11.84DD90 pKa = 4.58SYY92 pKa = 12.21DD93 pKa = 3.31PVQCLWGRR101 pKa = 11.84AIIVGMKK108 pKa = 9.98

Molecular weight: 12.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

12956

59

1280

264.4

28.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.72 ± 0.601

0.679 ± 0.132

5.966 ± 0.235

5.357 ± 0.284

3.141 ± 0.169

7.842 ± 0.294

2.123 ± 0.222

4.168 ± 0.223

4.515 ± 0.228

8.359 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.833 ± 0.18

3.605 ± 0.2

4.701 ± 0.342

4.893 ± 0.331

6.522 ± 0.289

5.195 ± 0.3

6.005 ± 0.404

6.993 ± 0.273

1.443 ± 0.135

2.941 ± 0.212
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski