Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Granulibacter; Granulibacter bethesdensis

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2565 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0BSF8|Q0BSF8_GRABC Hemagglutinin-related protein OS=Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) OX=391165 GN=GbCGDNIH1_1346 PE=4 SV=1
MM1 pKa = 7.01TTTTTNSGTISTGIVTVSGSEE22 pKa = 3.88IYY24 pKa = 10.78TNTTTGLITVPVTGTEE40 pKa = 4.01YY41 pKa = 10.85GAGIINSGTILNNAASTGATTAVSLNDD68 pKa = 3.56TGSVAGSATNIDD80 pKa = 4.06NNQGALIQGTDD91 pKa = 3.25YY92 pKa = 11.66GLDD95 pKa = 3.18IEE97 pKa = 4.93AASSSLTLSITNQGTILGGAGDD119 pKa = 4.31GSTNGVAIYY128 pKa = 10.12GIANTTLVNNTGGVISGDD146 pKa = 3.34DD147 pKa = 3.1TGIRR151 pKa = 11.84LGVPAANTSPVSYY164 pKa = 10.58SITNYY169 pKa = 8.75GTITGINNTGIDD181 pKa = 3.93LASGGTVTNNHH192 pKa = 5.78GAVIRR197 pKa = 11.84GYY199 pKa = 8.26WAGVAMVANSSHH211 pKa = 6.5NSVTTGGTVITDD223 pKa = 3.29GTIIGQNNIAIQLAAPVSMEE243 pKa = 4.09PYY245 pKa = 10.1SNQVQIGTDD254 pKa = 3.2AVFEE258 pKa = 4.71GIVDD262 pKa = 4.0GGVPASSTVASSALVLMAATSSDD285 pKa = 3.46TNKK288 pKa = 9.63TGKK291 pKa = 10.4LSGIGTEE298 pKa = 4.39FVNFSALSLANGAGWIIDD316 pKa = 3.74GGLDD320 pKa = 3.73SISTVSLGAASEE332 pKa = 4.16LTLAGTGTLTSSQTIAFNNGGSRR355 pKa = 11.84LRR357 pKa = 11.84LGTGYY362 pKa = 11.32ALNAGNLQLGQGADD376 pKa = 3.89TIQVADD382 pKa = 3.46QSFNLTAGSGALTFIAGSGKK402 pKa = 8.38STITGGSGALTFTGGSGASTVTSGAGGGTLSGGSDD437 pKa = 3.27GGNLLVAGGGNTQLRR452 pKa = 11.84AAGNGDD458 pKa = 3.56QLFGASHH465 pKa = 6.82GSSTLIGAANGQVSLVGGTGTALIVAGSGGGAIYY499 pKa = 10.4AGSGSSTVFGSNAGGLVGTGTGNSLLVNMGRR530 pKa = 11.84STIAGEE536 pKa = 4.42TGTSTIFGSQSGQNTVFGGTGAMQLAEE563 pKa = 4.3TGGSNLVMGGGGAMTLFGGVGNDD586 pKa = 4.27TILTGQGGIMTAFLGGNQNLVGLGSANTTVVAGSGSDD623 pKa = 3.58VFAVTAGAGGGTTTIYY639 pKa = 11.08DD640 pKa = 4.23FDD642 pKa = 4.36PSKK645 pKa = 11.42DD646 pKa = 3.49KK647 pKa = 11.04IGLFGYY653 pKa = 9.9QPSEE657 pKa = 3.44ISQNVTNGSLQISLSDD673 pKa = 3.27HH674 pKa = 5.26TVITLSGVTNAQGSILATT692 pKa = 3.66

Molecular weight: 66.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0BUA3|Q0BUA3_GRABC Ribonuclease D OS=Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) OX=391165 GN=GbCGDNIH1_0701 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.32VIANRR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.46GRR39 pKa = 11.84KK40 pKa = 8.72RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2565

0

2565

840892

40

4073

327.8

35.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.998 ± 0.069

0.977 ± 0.019

5.383 ± 0.046

5.091 ± 0.061

3.363 ± 0.032

8.75 ± 0.092

2.447 ± 0.031

5.211 ± 0.051

2.672 ± 0.042

10.423 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.632 ± 0.022

2.656 ± 0.069

5.565 ± 0.057

3.502 ± 0.032

7.321 ± 0.067

6.008 ± 0.07

5.572 ± 0.065

6.935 ± 0.038

1.384 ± 0.021

2.112 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski