Caballeronia mineralivorans PML1(12)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Caballeronia; Caballeronia mineralivorans

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8182 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J1D1M2|A0A0J1D1M2_9BURK Sulfate ABC transporter substrate-binding protein OS=Caballeronia mineralivorans PML1(12) OX=908627 GN=EOS_08750 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.52LILEE6 pKa = 4.41ARR8 pKa = 11.84HH9 pKa = 6.2LFDD12 pKa = 4.66GSVAAVAHH20 pKa = 5.49TTTHH24 pKa = 6.8SDD26 pKa = 3.4TADD29 pKa = 3.68HH30 pKa = 6.18GHH32 pKa = 6.64HH33 pKa = 6.68PVSSDD38 pKa = 2.72LSHH41 pKa = 6.46PHH43 pKa = 5.85SRR45 pKa = 11.84HH46 pKa = 5.55YY47 pKa = 11.19DD48 pKa = 3.54QTQHH52 pKa = 7.25DD53 pKa = 4.32ATLTPTYY60 pKa = 10.86APGDD64 pKa = 3.86IPALAPNPAATEE76 pKa = 3.81ILFIDD81 pKa = 3.75PRR83 pKa = 11.84VANWQALAKK92 pKa = 10.08GVAKK96 pKa = 10.35NVQIVLVDD104 pKa = 3.79PNRR107 pKa = 11.84DD108 pKa = 3.01GLEE111 pKa = 3.95QVTAALKK118 pKa = 10.56GRR120 pKa = 11.84SDD122 pKa = 3.25LTAIQFLTYY131 pKa = 9.59GQSGQIEE138 pKa = 4.97LGNAPITTATLASHH152 pKa = 6.78AGEE155 pKa = 4.05VASWGDD161 pKa = 3.54HH162 pKa = 5.43LAANADD168 pKa = 3.64IEE170 pKa = 4.72FWGCDD175 pKa = 2.93VGQGDD180 pKa = 3.79NGLAFVDD187 pKa = 4.5SVHH190 pKa = 6.81ALTGAQVAASTNATGAAQLGGDD212 pKa = 3.5WTLEE216 pKa = 3.85RR217 pKa = 11.84TTGVLAVGAPFSAASMAAYY236 pKa = 9.9QGVLDD241 pKa = 4.31SPVPTVSFDD250 pKa = 3.48PATVPGDD257 pKa = 3.59VLLGSTFTEE266 pKa = 4.27TVTFANAATNGVGYY280 pKa = 10.73GPFIDD285 pKa = 5.61LYY287 pKa = 9.89VPKK290 pKa = 10.38DD291 pKa = 3.38AAQTATLTGASFLGAGITPEE311 pKa = 4.13AVTLSTTIPGHH322 pKa = 5.41VGVLGALHH330 pKa = 7.17PLALDD335 pKa = 3.48NTGQPLFVAAPPGYY349 pKa = 9.3QANDD353 pKa = 3.03VMYY356 pKa = 10.69VLSLPFGSFTPGQPAAQIQLTFTLDD381 pKa = 3.13NSTEE385 pKa = 3.95LSSMHH390 pKa = 6.75GGQALDD396 pKa = 3.22ITAIGGFQYY405 pKa = 10.94GADD408 pKa = 3.97PLNDD412 pKa = 4.29PGTDD416 pKa = 3.32PSIRR420 pKa = 11.84GTAGTAGVTSAADD433 pKa = 3.56GMAVAASTVSLLDD446 pKa = 3.4VSATTDD452 pKa = 3.18LHH454 pKa = 6.13EE455 pKa = 5.16SEE457 pKa = 4.91TATGPDD463 pKa = 3.36YY464 pKa = 11.0PFNYY468 pKa = 10.15VLTIKK473 pKa = 9.77PAPVTQGDD481 pKa = 4.14PLSNTTFTFDD491 pKa = 4.72LPPEE495 pKa = 4.03VQYY498 pKa = 9.62THH500 pKa = 5.78GTIAFTGPGGATGTATFNPGVAGANGPGGTVTVHH534 pKa = 5.46FTSLGTDD541 pKa = 3.54GADD544 pKa = 2.88TATVVKK550 pKa = 10.35IPVFVPQFDD559 pKa = 4.71ANGNPVLDD567 pKa = 3.94AAGTPRR573 pKa = 11.84DD574 pKa = 3.37ITAATIYY581 pKa = 9.99SYY583 pKa = 10.46TGSWTPTAASLDD595 pKa = 3.78HH596 pKa = 6.91ALGVQTISGDD606 pKa = 3.3SSTNPTSTDD615 pKa = 3.65FVAKK619 pKa = 10.31ALAIQVTDD627 pKa = 4.47DD628 pKa = 3.86APAGNIVPGEE638 pKa = 4.15VVTEE642 pKa = 4.41SIHH645 pKa = 7.48FEE647 pKa = 3.8VSDD650 pKa = 4.25YY651 pKa = 11.73YY652 pKa = 11.6SLNQLKK658 pKa = 10.21IADD661 pKa = 3.87QLGDD665 pKa = 3.94GLSLLSPTEE674 pKa = 3.99LGYY677 pKa = 10.48VAPTLSVTRR686 pKa = 11.84SGVSSAPISFGDD698 pKa = 3.67AGNLVAATVNGQAVAEE714 pKa = 4.39SGSNSAWNYY723 pKa = 8.32TRR725 pKa = 11.84DD726 pKa = 4.43DD727 pKa = 4.31AGTSANPGATSISFGVGALLEE748 pKa = 4.32AQMGAGALASVLQGGGGGATQGVITFVTKK777 pKa = 10.82VLDD780 pKa = 4.15KK781 pKa = 10.41YY782 pKa = 9.56TNTNNEE788 pKa = 3.54DD789 pKa = 3.47SLRR792 pKa = 11.84EE793 pKa = 3.69KK794 pKa = 10.91DD795 pKa = 3.56QITNQVTLAGTSASVVTVDD814 pKa = 3.76NTAQTVGASTGTVADD829 pKa = 3.99NSSVTDD835 pKa = 3.74TVAPGSMVLSVVAVNGQTTDD855 pKa = 3.28LTHH858 pKa = 7.03IEE860 pKa = 4.33PGDD863 pKa = 3.67TVTYY867 pKa = 10.36GLTYY871 pKa = 10.93SLTTGDD877 pKa = 4.39YY878 pKa = 11.37GSLDD882 pKa = 3.44LTANLPLPVFSTTDD896 pKa = 3.39PTSTGSNVTAYY907 pKa = 9.18TADD910 pKa = 3.38NTADD914 pKa = 3.65SFPTAGTYY922 pKa = 10.88KK923 pKa = 10.77LINPLSGEE931 pKa = 4.45SVVSATANGTANSISFNFGNRR952 pKa = 11.84DD953 pKa = 3.43DD954 pKa = 4.05TTNAAGQKK962 pKa = 7.04VTVYY966 pKa = 9.63FSVVASNKK974 pKa = 9.39PFADD978 pKa = 3.98GLPLTSQGDD987 pKa = 3.72SSYY990 pKa = 11.6TNAEE994 pKa = 3.94GQTAATAAIQQVPLEE1009 pKa = 4.26EE1010 pKa = 4.61PEE1012 pKa = 4.3VTVKK1016 pKa = 10.13TGVVSVVGDD1025 pKa = 3.86GSASKK1030 pKa = 9.49GTFTPDD1036 pKa = 2.71TSNPNSAIPWTPQTDD1051 pKa = 3.84VTPSGAAGSPFAPAGTPAGTLFTTVGANGTANPLAADD1088 pKa = 4.23DD1089 pKa = 4.94LNVTGADD1096 pKa = 3.29GGDD1099 pKa = 3.16ITRR1102 pKa = 11.84VVSTVDD1108 pKa = 3.51NEE1110 pKa = 4.17GHH1112 pKa = 6.9ASAFDD1117 pKa = 3.54VTVQGTLPSGYY1128 pKa = 8.64STGNVTNFAIYY1139 pKa = 10.47NSAGAQIDD1147 pKa = 3.81TGVTAAQYY1155 pKa = 9.96FSAGGVKK1162 pKa = 10.52LISTAGTDD1170 pKa = 3.26VGIAAGDD1177 pKa = 3.6RR1178 pKa = 11.84VYY1180 pKa = 11.22VVYY1183 pKa = 10.81DD1184 pKa = 3.58LTLSTTQQTGEE1195 pKa = 4.06TLAAGSSVVNWASVTGGVAAGNGFVSGTGTAALTLGEE1232 pKa = 4.23GSGALSDD1239 pKa = 3.82SATIGISAPSVTKK1252 pKa = 10.02TVTSGSDD1259 pKa = 2.88TALPLGTDD1267 pKa = 3.28NAVVPGEE1274 pKa = 4.32TVTYY1278 pKa = 9.18TVVLTLPQGVTSNGASEE1295 pKa = 4.36LTLTDD1300 pKa = 3.5QLPAGMTFGSISSTSFGTGVTSSNGGTITASSSGSTVTFDD1340 pKa = 4.39LGSSVTNSQVDD1351 pKa = 3.7TNGTITIVYY1360 pKa = 6.94TATVDD1365 pKa = 3.8ATDD1368 pKa = 4.1TPHH1371 pKa = 7.03GGAPTTYY1378 pKa = 10.57SNSAFLNYY1386 pKa = 10.22DD1387 pKa = 3.36SGQTTPVTAANTATVTEE1404 pKa = 4.84HH1405 pKa = 7.07DD1406 pKa = 3.98PQVIEE1411 pKa = 4.91TITAADD1417 pKa = 3.82TTTGVPIATNGTVFSHH1433 pKa = 6.82EE1434 pKa = 4.1GLTYY1438 pKa = 10.42TVTLTNNGTAPANNLADD1455 pKa = 5.26LLDD1458 pKa = 4.34LPAGLTYY1465 pKa = 11.18VNGSLQHH1472 pKa = 5.95VSGGTNSTTSDD1483 pKa = 3.36ANASALNIGLDD1494 pKa = 3.7TLAVGQTATFTFQATVNPNLPAGTSFTVDD1523 pKa = 3.18TPADD1527 pKa = 4.01TTSGTYY1533 pKa = 10.38YY1534 pKa = 10.51SLPGTAQGHH1543 pKa = 6.23KK1544 pKa = 9.91YY1545 pKa = 10.26SDD1547 pKa = 3.79SATDD1551 pKa = 3.23TATIGQITPVLSITGEE1567 pKa = 4.24SNNTDD1572 pKa = 3.3TASTPTQTNTQTSTEE1587 pKa = 4.09AAVGEE1592 pKa = 4.67VVTMQAYY1599 pKa = 9.76VEE1601 pKa = 4.28IPEE1604 pKa = 4.53GSNPTTLNFTMPSGLEE1620 pKa = 3.84YY1621 pKa = 11.06LNDD1624 pKa = 3.78GSAKK1628 pKa = 9.87IAFVSPNGDD1637 pKa = 3.32LVSTDD1642 pKa = 3.47SALSGITQYY1651 pKa = 11.57QDD1653 pKa = 3.12INPGAANYY1661 pKa = 9.27ISPSAITNTGAGTVDD1676 pKa = 3.14VATFRR1681 pKa = 11.84PVDD1684 pKa = 3.82ALPGSVVTATGNSVAIDD1701 pKa = 4.25LGTLSNNDD1709 pKa = 3.02NSSTGNYY1716 pKa = 9.7VLVEE1720 pKa = 4.51FNAVVTNVAANRR1732 pKa = 11.84QGASALQTSFTVAGVTSNDD1751 pKa = 2.7VSVAVEE1757 pKa = 4.41EE1758 pKa = 4.36PAVTVTKK1765 pKa = 9.74TATAVDD1771 pKa = 3.91NTNDD1775 pKa = 3.24TVTYY1779 pKa = 9.95QVTVKK1784 pKa = 9.52NTGLSSAYY1792 pKa = 10.32NVVLDD1797 pKa = 4.7NPAATNEE1804 pKa = 4.14SNIAFVSATGAGTGGTGAGGTTGTDD1829 pKa = 2.94LNYY1832 pKa = 10.85AIGQLGAGGSEE1843 pKa = 4.11VITYY1847 pKa = 6.94TVKK1850 pKa = 10.28VAPGQTVQNDD1860 pKa = 3.48TATATWQSLAGQQTFNGSTAGAVGSVTGPRR1890 pKa = 11.84DD1891 pKa = 3.45FDD1893 pKa = 3.98PAANPPNNYY1902 pKa = 9.41VATAATDD1909 pKa = 2.96IGSAQGRR1916 pKa = 11.84VWQDD1920 pKa = 2.83VGHH1923 pKa = 7.34DD1924 pKa = 3.49KK1925 pKa = 8.59TTFSQTGGSADD1936 pKa = 3.51TALGGITVTATITQPGGTVIHH1957 pKa = 6.35EE1958 pKa = 4.55TTTTASDD1965 pKa = 3.38GTYY1968 pKa = 10.84SFGALPDD1975 pKa = 3.54GTVAISLNGVPANEE1989 pKa = 4.11TLVYY1993 pKa = 10.72DD1994 pKa = 4.06PDD1996 pKa = 4.84GSVSSSPPSASFTSAGDD2013 pKa = 3.26AHH2015 pKa = 6.53NSVDD2019 pKa = 4.92FSFQAPDD2026 pKa = 3.57TAPVIANWSGSTTYY2040 pKa = 11.29AEE2042 pKa = 4.87GGPAMVLSGAGASVSDD2058 pKa = 3.94TQLDD2062 pKa = 3.87ALGGDD2067 pKa = 4.09YY2068 pKa = 11.33SNTTLTLQRR2077 pKa = 11.84YY2078 pKa = 8.33SGGVAAPVNTDD2089 pKa = 3.16VFVGTGLLSLSGSTVTFSGVAVGTFAEE2116 pKa = 4.52ANGKK2120 pKa = 10.13LSITFGAGATAATVKK2135 pKa = 10.69NVLDD2139 pKa = 3.63SLGYY2143 pKa = 10.44SSTDD2147 pKa = 2.79TSTVSTGIQIGATLDD2162 pKa = 3.49DD2163 pKa = 5.13HH2164 pKa = 6.27NTAGAQGTGGNMTSVPAFVTVNEE2187 pKa = 4.21IPAPGGSSATFIEE2200 pKa = 5.09PNDD2203 pKa = 3.73SPAAAVAVAVDD2214 pKa = 3.89STLSVASSDD2223 pKa = 3.62TFAGATVKK2231 pKa = 10.46ISNFKK2236 pKa = 10.17PGEE2239 pKa = 4.18DD2240 pKa = 3.42VLVAGALPSGVTASFDD2256 pKa = 3.57AATGTMTLTGTNLSAATVQAALRR2279 pKa = 11.84GVSYY2283 pKa = 10.82YY2284 pKa = 10.9DD2285 pKa = 4.49SSDD2288 pKa = 3.61TPNTTVRR2295 pKa = 11.84DD2296 pKa = 3.85VTISVMDD2303 pKa = 3.64STTNATTTAAVATIDD2318 pKa = 3.76VVATNDD2324 pKa = 3.49SPLLNGVPVTLNHH2337 pKa = 5.84ATEE2340 pKa = 4.57DD2341 pKa = 3.13AGAPVGAVGTLVSALTGSGNVTDD2364 pKa = 4.65PDD2366 pKa = 3.64GANAHH2371 pKa = 7.08DD2372 pKa = 4.4GTSPGAIGVAITAANTTVGTWWYY2395 pKa = 8.34STNNGATWTEE2405 pKa = 3.91FAGNGTTAVSEE2416 pKa = 4.28ANALHH2421 pKa = 6.68LVADD2425 pKa = 4.61GNTRR2429 pKa = 11.84VYY2431 pKa = 10.74FDD2433 pKa = 5.44PAPDD2437 pKa = 3.55WNGSVPNALTFRR2449 pKa = 11.84AWDD2452 pKa = 3.68QFDD2455 pKa = 3.51GAANGSLSALPAANTLGQGLNTLGSAYY2482 pKa = 10.48SSASQSVDD2490 pKa = 4.31LIGDD2494 pKa = 4.12PVNDD2498 pKa = 3.82APIASGSTTLGSAAEE2513 pKa = 4.09DD2514 pKa = 3.59TAAA2517 pKa = 4.75

Molecular weight: 251.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J1CR47|A0A0J1CR47_9BURK DEAD/DEAH box helicase OS=Caballeronia mineralivorans PML1(12) OX=908627 GN=EOS_27170 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.74TAGGRR28 pKa = 11.84KK29 pKa = 9.04VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8182

0

8182

2499026

24

4234

305.4

33.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.116 ± 0.031

0.906 ± 0.008

5.382 ± 0.022

5.108 ± 0.026

3.825 ± 0.017

8.114 ± 0.033

2.264 ± 0.014

5.029 ± 0.016

3.289 ± 0.023

10.285 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.424 ± 0.012

2.928 ± 0.019

4.938 ± 0.017

3.549 ± 0.018

6.631 ± 0.03

5.998 ± 0.024

5.6 ± 0.026

7.852 ± 0.017

1.328 ± 0.011

2.434 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski