BtRf-AlphaCoV/YN2012

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Rhinacovirus; unclassified Rhinacovirus

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

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Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0U1WHF9|A0A0U1WHF9_9ALPC Nucleoprotein OS=BtRf-AlphaCoV/YN2012 OX=1503293 PE=4 SV=1
MM1 pKa = 7.81SINQLTLAVASDD13 pKa = 3.95QEE15 pKa = 4.56ISAHH19 pKa = 6.3GYY21 pKa = 8.13STMSDD26 pKa = 3.15AVEE29 pKa = 4.56HH30 pKa = 6.26FSSSASHH37 pKa = 6.38GFKK40 pKa = 10.4DD41 pKa = 3.59CRR43 pKa = 11.84FVAFGLQDD51 pKa = 3.07IVIGVEE57 pKa = 3.64PSDD60 pKa = 3.47FVVALEE66 pKa = 4.13GDD68 pKa = 4.45EE69 pKa = 4.88ILTAYY74 pKa = 9.69IATFGARR81 pKa = 11.84PRR83 pKa = 11.84CLRR86 pKa = 11.84GWLIPSNSNYY96 pKa = 9.93VLEE99 pKa = 4.65DD100 pKa = 3.52FQVIFGKK107 pKa = 10.61RR108 pKa = 11.84GGNVVFVDD116 pKa = 4.47NYY118 pKa = 10.37MCGADD123 pKa = 4.2GKK125 pKa = 9.55PAVPRR130 pKa = 11.84EE131 pKa = 3.74QWSFEE136 pKa = 3.88EE137 pKa = 5.28HH138 pKa = 6.78FDD140 pKa = 5.45DD141 pKa = 4.67DD142 pKa = 4.32TEE144 pKa = 5.42DD145 pKa = 3.76IIINGVTYY153 pKa = 9.97RR154 pKa = 11.84HH155 pKa = 5.82AWNTIRR161 pKa = 11.84ADD163 pKa = 3.89DD164 pKa = 4.61PYY166 pKa = 10.8EE167 pKa = 4.21KK168 pKa = 10.31QGLLSIKK175 pKa = 10.13SIEE178 pKa = 4.22YY179 pKa = 10.53VSTIPHH185 pKa = 7.11KK186 pKa = 10.79LPNGSVLSVAGPPKK200 pKa = 9.84KK201 pKa = 10.23RR202 pKa = 11.84KK203 pKa = 9.64AVVLDD208 pKa = 3.45EE209 pKa = 5.04KK210 pKa = 11.36YY211 pKa = 10.98NKK213 pKa = 9.67IYY215 pKa = 9.97NACGVPFVTNGKK227 pKa = 9.77SISEE231 pKa = 4.42VVTKK235 pKa = 10.11PLFLHH240 pKa = 6.82ALVKK244 pKa = 9.76CKK246 pKa = 10.34CGNEE250 pKa = 3.8SWTVGDD256 pKa = 3.14WTGFKK261 pKa = 7.54TTCCGATGKK270 pKa = 8.48VTTFAVGDD278 pKa = 3.83VTPGDD283 pKa = 3.33IVFTTTGAGTGTKK296 pKa = 9.29FFCGLVLTHH305 pKa = 6.86VDD307 pKa = 3.27TLEE310 pKa = 4.43GVSAWRR316 pKa = 11.84VVKK319 pKa = 10.49AYY321 pKa = 9.29TVDD324 pKa = 3.43TFVASSNFDD333 pKa = 3.39EE334 pKa = 5.3FNHH337 pKa = 5.65VMSLDD342 pKa = 3.28QCSFDD347 pKa = 3.67NFSSISVALKK357 pKa = 9.9FSLLRR362 pKa = 11.84GSCCDD367 pKa = 3.54DD368 pKa = 3.41VKK370 pKa = 11.31VAVATGVIDD379 pKa = 3.21IGLGVFDD386 pKa = 4.56VNDD389 pKa = 3.82TVFEE393 pKa = 4.07NVPWFVQKK401 pKa = 10.93FEE403 pKa = 4.18FLKK406 pKa = 10.51PAWDD410 pKa = 3.75ALKK413 pKa = 10.49QAIINLGVTSKK424 pKa = 11.15AVLSFIKK431 pKa = 10.08SLCSAAFSVVDD442 pKa = 4.01GVPVIVCTVAEE453 pKa = 4.24RR454 pKa = 11.84FSKK457 pKa = 10.97AFTDD461 pKa = 4.03FLSYY465 pKa = 10.35TRR467 pKa = 11.84EE468 pKa = 4.24CFTALCDD475 pKa = 4.72DD476 pKa = 3.52IVVFGVNCKK485 pKa = 10.31AVGDD489 pKa = 3.79YY490 pKa = 10.61IIFNNSVVKK499 pKa = 9.72TIKK502 pKa = 10.82AKK504 pKa = 10.26VKK506 pKa = 9.36GVKK509 pKa = 9.2EE510 pKa = 3.97AGLKK514 pKa = 9.82NVTYY518 pKa = 7.13TQCLLGPTKK527 pKa = 10.18SVKK530 pKa = 9.66VKK532 pKa = 10.31RR533 pKa = 11.84VEE535 pKa = 4.11SSVAQLKK542 pKa = 10.07VVDD545 pKa = 3.76TAVPLKK551 pKa = 10.68QEE553 pKa = 4.38GEE555 pKa = 4.35CLVVGGRR562 pKa = 11.84AMFRR566 pKa = 11.84SDD568 pKa = 2.67GYY570 pKa = 10.96YY571 pKa = 10.45RR572 pKa = 11.84FMADD576 pKa = 2.72ADD578 pKa = 4.15VVLEE582 pKa = 4.38SPVFTAGSQYY592 pKa = 11.37NVVFEE597 pKa = 4.12TDD599 pKa = 3.21ALFIAPEE606 pKa = 3.92IGTVFEE612 pKa = 4.98GDD614 pKa = 3.71NIDD617 pKa = 3.65TVVQIVANKK626 pKa = 8.96VQAFNNRR633 pKa = 11.84FVVYY637 pKa = 8.69NTHH640 pKa = 6.29IVDD643 pKa = 3.82GAITVFCDD651 pKa = 3.51YY652 pKa = 11.13NFSCPAFLDD661 pKa = 4.51GFPEE665 pKa = 4.0WFSFCKK671 pKa = 10.45NHH673 pKa = 6.47FKK675 pKa = 10.88DD676 pKa = 3.14AGFVEE681 pKa = 5.96FYY683 pKa = 11.27DD684 pKa = 4.78SIINSDD690 pKa = 4.07SNFQTTFQPYY700 pKa = 10.51LDD702 pKa = 4.59LKK704 pKa = 11.08SSIDD708 pKa = 3.43AYY710 pKa = 10.85LGPEE714 pKa = 3.69IVRR717 pKa = 11.84AVDD720 pKa = 4.23GGRR723 pKa = 11.84LWRR726 pKa = 11.84TVINGISDD734 pKa = 3.46AVNFCKK740 pKa = 10.39NLRR743 pKa = 11.84LHH745 pKa = 6.95FEE747 pKa = 3.9FGEE750 pKa = 4.25LKK752 pKa = 9.32GTVVKK757 pKa = 10.47RR758 pKa = 11.84FKK760 pKa = 11.05GILRR764 pKa = 11.84TLLTVYY770 pKa = 10.9NEE772 pKa = 4.89FIQTTVSVLTICGISAKK789 pKa = 10.32YY790 pKa = 9.77YY791 pKa = 10.94AFDD794 pKa = 3.78KK795 pKa = 11.06PMLCMHH801 pKa = 7.63GITEE805 pKa = 4.58RR806 pKa = 11.84VTSFDD811 pKa = 2.97ITNLGKK817 pKa = 9.82PILDD821 pKa = 3.87GLSKK825 pKa = 11.1CNVFACGSNAVVVNSVDD842 pKa = 4.2HH843 pKa = 6.62EE844 pKa = 4.24LLDD847 pKa = 4.32LEE849 pKa = 4.32EE850 pKa = 5.47CEE852 pKa = 4.41FVEE855 pKa = 4.51PASHH859 pKa = 6.16GVLVISDD866 pKa = 4.14DD867 pKa = 3.73YY868 pKa = 11.92GFYY871 pKa = 10.58SCEE874 pKa = 3.86TGIYY878 pKa = 9.66PYY880 pKa = 11.19ASDD883 pKa = 4.4GKK885 pKa = 10.27VLPLRR890 pKa = 11.84FRR892 pKa = 11.84KK893 pKa = 9.67KK894 pKa = 10.46AGGKK898 pKa = 9.08NVAFDD903 pKa = 4.38DD904 pKa = 4.05NVIVTEE910 pKa = 4.17IDD912 pKa = 3.31PVFKK916 pKa = 10.99VKK918 pKa = 10.94LNFEE922 pKa = 4.23FDD924 pKa = 3.56DD925 pKa = 4.64DD926 pKa = 4.45KK927 pKa = 11.72LVTICEE933 pKa = 4.11KK934 pKa = 11.16VIGNKK939 pKa = 9.29IKK941 pKa = 10.64CSSWSQLVEE950 pKa = 4.38IIEE953 pKa = 4.39TALTTIKK960 pKa = 10.24SYY962 pKa = 11.2YY963 pKa = 9.71SVPIYY968 pKa = 10.36YY969 pKa = 9.59IYY971 pKa = 10.61DD972 pKa = 3.59EE973 pKa = 4.98EE974 pKa = 6.1GGEE977 pKa = 4.16DD978 pKa = 3.68LSVPIMISEE987 pKa = 4.29WPLDD991 pKa = 3.68STGTEE996 pKa = 3.44IVVDD1000 pKa = 3.98VEE1002 pKa = 5.35DD1003 pKa = 3.42VDD1005 pKa = 4.85EE1006 pKa = 4.61PVSQIEE1012 pKa = 4.16EE1013 pKa = 4.21VNNAFAIEE1021 pKa = 4.16PSPKK1025 pKa = 9.22RR1026 pKa = 11.84DD1027 pKa = 3.32VSPFGFDD1034 pKa = 3.11SHH1036 pKa = 7.07DD1037 pKa = 3.74LNGIRR1042 pKa = 11.84VLVQSQNNCWVNATCYY1058 pKa = 9.02QLQMLGYY1065 pKa = 9.73DD1066 pKa = 3.76DD1067 pKa = 3.92TAMEE1071 pKa = 4.84LFRR1074 pKa = 11.84HH1075 pKa = 5.95GGVHH1079 pKa = 6.51NLVKK1083 pKa = 10.28RR1084 pKa = 11.84CYY1086 pKa = 8.76EE1087 pKa = 3.76VTGAFLGSLGDD1098 pKa = 3.4VSFCLEE1104 pKa = 3.76EE1105 pKa = 4.09LLRR1108 pKa = 11.84DD1109 pKa = 3.51VKK1111 pKa = 9.78MAKK1114 pKa = 8.71VTVEE1118 pKa = 4.06VTCNCSSYY1126 pKa = 10.17MDD1128 pKa = 4.2EE1129 pKa = 3.99LHH1131 pKa = 5.97GTFFRR1136 pKa = 11.84FMPSKK1141 pKa = 10.71QKK1143 pKa = 10.68FEE1145 pKa = 4.19YY1146 pKa = 10.02GGCGEE1151 pKa = 4.48CHH1153 pKa = 4.88GTRR1156 pKa = 11.84YY1157 pKa = 10.23YY1158 pKa = 10.04RR1159 pKa = 11.84VCGIVGSAIFAQPLKK1174 pKa = 10.7HH1175 pKa = 6.62LNLDD1179 pKa = 3.95DD1180 pKa = 4.59LVCDD1184 pKa = 3.73VASASVFLGNDD1195 pKa = 3.34CGHH1198 pKa = 6.08YY1199 pKa = 9.56MINDD1203 pKa = 3.87YY1204 pKa = 8.5VHH1206 pKa = 7.15KK1207 pKa = 10.4ICVDD1211 pKa = 2.99GMGVYY1216 pKa = 10.13KK1217 pKa = 10.42IKK1219 pKa = 10.86HH1220 pKa = 4.72NTIDD1224 pKa = 3.65TIVAKK1229 pKa = 10.29NANIKK1234 pKa = 10.09PNIIAPFTEE1243 pKa = 4.02YY1244 pKa = 11.11CNIKK1248 pKa = 10.13FYY1250 pKa = 11.06QGDD1253 pKa = 3.91FKK1255 pKa = 11.62DD1256 pKa = 3.92LAGLSHH1262 pKa = 7.3DD1263 pKa = 4.06FVVNAANCNLAHH1275 pKa = 6.74GGGVAKK1281 pKa = 10.66AINDD1285 pKa = 3.64YY1286 pKa = 11.07TDD1288 pKa = 3.46GKK1290 pKa = 9.87LQKK1293 pKa = 10.35LSNSFVKK1300 pKa = 10.74KK1301 pKa = 10.16NGKK1304 pKa = 9.44VDD1306 pKa = 3.33VGEE1309 pKa = 4.35CVMLDD1314 pKa = 3.11TGKK1317 pKa = 10.37IKK1319 pKa = 10.62VLNAVGPRR1327 pKa = 11.84KK1328 pKa = 10.39GKK1330 pKa = 10.02DD1331 pKa = 3.02AEE1333 pKa = 4.37SLLNTVYY1340 pKa = 11.01TNIFSRR1346 pKa = 11.84KK1347 pKa = 8.62GVPLMPLISCGIFGFDD1363 pKa = 4.69LKK1365 pKa = 11.25DD1366 pKa = 3.36SLRR1369 pKa = 11.84AFLDD1373 pKa = 3.41ACGNRR1378 pKa = 11.84HH1379 pKa = 5.32VKK1381 pKa = 10.35CFVYY1385 pKa = 10.72SDD1387 pKa = 4.07LEE1389 pKa = 4.11RR1390 pKa = 11.84EE1391 pKa = 4.23AVMKK1395 pKa = 10.43FLTTPCDD1402 pKa = 3.53DD1403 pKa = 3.6TVVSVEE1409 pKa = 4.36TVGTPLIEE1417 pKa = 4.05EE1418 pKa = 4.3TTVNVVPPFRR1428 pKa = 11.84TIGTHH1433 pKa = 5.19NFYY1436 pKa = 10.76DD1437 pKa = 4.34FSGVEE1442 pKa = 4.31PILGLGVKK1450 pKa = 10.34NIVLFTDD1457 pKa = 3.26QVLSVKK1463 pKa = 10.68DD1464 pKa = 3.58FGVVVDD1470 pKa = 3.67RR1471 pKa = 11.84HH1472 pKa = 6.3LNGLLSDD1479 pKa = 4.05MSSKK1483 pKa = 10.63YY1484 pKa = 10.85VNDD1487 pKa = 3.63HH1488 pKa = 6.11KK1489 pKa = 11.06AVPFGNILSLDD1500 pKa = 3.71CGAFTVVDD1508 pKa = 3.68AVTPFEE1514 pKa = 4.23GASFEE1519 pKa = 4.33KK1520 pKa = 10.68QSGRR1524 pKa = 11.84TIRR1527 pKa = 11.84KK1528 pKa = 6.99LTRR1531 pKa = 11.84LDD1533 pKa = 3.64GSTLCVLPNNSALFDD1548 pKa = 3.77KK1549 pKa = 11.06LFALGDD1555 pKa = 3.64NFSFLVLDD1563 pKa = 3.54NVKK1566 pKa = 10.16PLFEE1570 pKa = 6.42DD1571 pKa = 3.6YY1572 pKa = 11.01LKK1574 pKa = 11.05PKK1576 pKa = 10.07DD1577 pKa = 3.66VRR1579 pKa = 11.84VKK1581 pKa = 9.33VTADD1585 pKa = 2.98GRR1587 pKa = 11.84NVNDD1591 pKa = 3.88VIVTTTEE1598 pKa = 3.72TFDD1601 pKa = 3.48AQLGPVSNGSEE1612 pKa = 4.12SLVGVTPTVADD1623 pKa = 3.32NGKK1626 pKa = 9.58VVNTVPDD1633 pKa = 4.11VNWSKK1638 pKa = 11.13HH1639 pKa = 4.8FGFSDD1644 pKa = 3.51AAAFAVLDD1652 pKa = 3.79HH1653 pKa = 7.0SKK1655 pKa = 10.43FAFDD1659 pKa = 4.9SEE1661 pKa = 4.39IVDD1664 pKa = 3.98GKK1666 pKa = 9.74RR1667 pKa = 11.84TLVDD1671 pKa = 3.39SDD1673 pKa = 3.89NNCWINATCLALQFLKK1689 pKa = 9.46PTFKK1693 pKa = 10.86YY1694 pKa = 10.71VGWEE1698 pKa = 3.93DD1699 pKa = 3.26LWNKK1703 pKa = 9.32FVTGDD1708 pKa = 3.21VSGFVHH1714 pKa = 7.02LLYY1717 pKa = 10.82YY1718 pKa = 10.19IEE1720 pKa = 5.31GVDD1723 pKa = 3.81KK1724 pKa = 10.88GAKK1727 pKa = 9.72GDD1729 pKa = 3.9VEE1731 pKa = 4.51STLSKK1736 pKa = 9.76LDD1738 pKa = 3.46KK1739 pKa = 10.81YY1740 pKa = 10.29IVSSGSVTIKK1750 pKa = 10.56RR1751 pKa = 11.84STLCEE1756 pKa = 4.16HH1757 pKa = 7.25CDD1759 pKa = 3.51STVKK1763 pKa = 10.12TMTGAIVEE1771 pKa = 4.3ASVILNGHH1779 pKa = 6.59IDD1781 pKa = 3.43GHH1783 pKa = 5.54CQHH1786 pKa = 6.41NFDD1789 pKa = 3.73WRR1791 pKa = 11.84VQVVGVKK1798 pKa = 10.52GDD1800 pKa = 3.69IILLHH1805 pKa = 6.42SGRR1808 pKa = 11.84ILEE1811 pKa = 4.19GPYY1814 pKa = 10.59LHH1816 pKa = 7.26GDD1818 pKa = 3.48AYY1820 pKa = 11.36VAFSGDD1826 pKa = 3.6TDD1828 pKa = 3.6NGHH1831 pKa = 5.54YY1832 pKa = 9.66TVFDD1836 pKa = 3.65NKK1838 pKa = 10.25LSKK1841 pKa = 10.37MYY1843 pKa = 10.77DD1844 pKa = 2.95GTKK1847 pKa = 10.13CVKK1850 pKa = 8.29TTLDD1854 pKa = 3.37TLTASSVVIRR1864 pKa = 11.84NGSYY1868 pKa = 9.94AVKK1871 pKa = 10.26SEE1873 pKa = 3.94HH1874 pKa = 6.39VPLIKK1879 pKa = 10.68KK1880 pKa = 10.3LDD1882 pKa = 3.91DD1883 pKa = 3.62SAEE1886 pKa = 4.01KK1887 pKa = 10.34FFNVGDD1893 pKa = 4.53IIAHH1897 pKa = 6.1NIAYY1901 pKa = 8.97FFVWLVTTLSTLYY1914 pKa = 10.36RR1915 pKa = 11.84CWRR1918 pKa = 11.84GGNFKK1923 pKa = 10.39LLSSIPEE1930 pKa = 3.75RR1931 pKa = 11.84SGVVLSRR1938 pKa = 11.84SLKK1941 pKa = 9.0YY1942 pKa = 10.49NFRR1945 pKa = 11.84ALRR1948 pKa = 11.84SNVVSKK1954 pKa = 10.77QKK1956 pKa = 9.99YY1957 pKa = 8.22CFMFAKK1963 pKa = 10.51LLLLLYY1969 pKa = 9.13TIYY1972 pKa = 11.48AMLFILVRR1980 pKa = 11.84FSFFNDD1986 pKa = 3.93YY1987 pKa = 10.9ICGTTVKK1994 pKa = 10.39GYY1996 pKa = 9.37TNSDD2000 pKa = 3.07FDD2002 pKa = 4.71KK2003 pKa = 11.65GKK2005 pKa = 10.05FCNGSLVCKK2014 pKa = 9.43TCLFGYY2020 pKa = 10.12QEE2022 pKa = 4.85LSDD2025 pKa = 4.04LQHH2028 pKa = 6.94VGVVWSYY2035 pKa = 10.21VRR2037 pKa = 11.84EE2038 pKa = 3.98PLFSSILPLCYY2049 pKa = 9.51FAVIAIFGGVIEE2061 pKa = 5.33RR2062 pKa = 11.84FALCYY2067 pKa = 9.56FAAQFINNTLIFFKK2081 pKa = 10.62LQDD2084 pKa = 4.03PFWPVQLVPFDD2095 pKa = 3.61IFGDD2099 pKa = 3.86EE2100 pKa = 3.93ILVIFLSYY2108 pKa = 10.71KK2109 pKa = 10.03AICFFKK2115 pKa = 10.72HH2116 pKa = 5.73VVFGCDD2122 pKa = 2.95KK2123 pKa = 9.55PNCIACCKK2131 pKa = 9.22SAKK2134 pKa = 9.46LKK2136 pKa = 10.49RR2137 pKa = 11.84IAMDD2141 pKa = 4.13TIVNGSRR2148 pKa = 11.84RR2149 pKa = 11.84SFYY2152 pKa = 11.51VNANGGAKK2160 pKa = 9.75LCKK2163 pKa = 9.47KK2164 pKa = 10.21HH2165 pKa = 6.84NFFCVACDD2173 pKa = 3.56SYY2175 pKa = 11.96GSGHH2179 pKa = 6.22TYY2181 pKa = 10.59INDD2184 pKa = 4.22HH2185 pKa = 6.78IATEE2189 pKa = 3.87LHH2191 pKa = 6.8NITKK2195 pKa = 10.08LHH2197 pKa = 5.72VKK2199 pKa = 8.26PTGPASINIDD2209 pKa = 2.8RR2210 pKa = 11.84VEE2212 pKa = 4.28FSDD2215 pKa = 3.41GFYY2218 pKa = 10.55RR2219 pKa = 11.84IFAGDD2224 pKa = 4.08KK2225 pKa = 7.66FWKK2228 pKa = 10.21YY2229 pKa = 11.26NFDD2232 pKa = 3.34ITDD2235 pKa = 3.36KK2236 pKa = 10.85KK2237 pKa = 10.16FSCRR2241 pKa = 11.84EE2242 pKa = 3.61VLKK2245 pKa = 10.91NCNILDD2251 pKa = 3.87DD2252 pKa = 6.07FIVFDD2257 pKa = 4.4NNGSNLSQVQNACVYY2272 pKa = 10.67LSQLLCKK2279 pKa = 9.35PIKK2282 pKa = 10.51IIDD2285 pKa = 3.84STLLSSLNVDD2295 pKa = 4.62FNGALHH2301 pKa = 7.02KK2302 pKa = 11.0AFLDD2306 pKa = 3.55VLHH2309 pKa = 6.89NSFGKK2314 pKa = 10.34DD2315 pKa = 3.45FSSCKK2320 pKa = 9.51TMHH2323 pKa = 6.85DD2324 pKa = 3.72CKK2326 pKa = 10.81EE2327 pKa = 3.98LLEE2330 pKa = 4.96LDD2332 pKa = 3.63ISDD2335 pKa = 3.64EE2336 pKa = 4.28DD2337 pKa = 3.98FVKK2340 pKa = 10.71VVSDD2344 pKa = 3.04AHH2346 pKa = 6.69RR2347 pKa = 11.84FDD2349 pKa = 4.72VLITDD2354 pKa = 4.09TSFNNFCTSYY2364 pKa = 11.08AKK2366 pKa = 10.32PSEE2369 pKa = 4.23KK2370 pKa = 10.45LSSFDD2375 pKa = 4.14LAHH2378 pKa = 7.0CMRR2381 pKa = 11.84SGAKK2385 pKa = 9.18VVNHH2389 pKa = 5.98NVLIKK2394 pKa = 10.53EE2395 pKa = 4.05KK2396 pKa = 10.44MPIVWNCVDD2405 pKa = 4.3FAKK2408 pKa = 10.81LSIDD2412 pKa = 2.98ARR2414 pKa = 11.84KK2415 pKa = 10.39YY2416 pKa = 8.93IVKK2419 pKa = 7.95TAKK2422 pKa = 10.36VKK2424 pKa = 10.64GVTFLLTINNNVMEE2438 pKa = 4.45TTLPCVSVLQKK2449 pKa = 10.64QGAGKK2454 pKa = 10.31LSSIWKK2460 pKa = 8.74HH2461 pKa = 4.86FWYY2464 pKa = 10.47ACGAILALFLLLNCLSFTEE2483 pKa = 4.42YY2484 pKa = 10.73AISLPGYY2491 pKa = 8.17DD2492 pKa = 3.76FKK2494 pKa = 11.57YY2495 pKa = 10.7IEE2497 pKa = 5.26DD2498 pKa = 4.04GQLKK2502 pKa = 9.93PFISGLYY2509 pKa = 9.05CVRR2512 pKa = 11.84NNFDD2516 pKa = 4.42TFMEE2520 pKa = 3.98WHH2522 pKa = 5.65AQKK2525 pKa = 10.82FGFRR2529 pKa = 11.84PSNSDD2534 pKa = 2.55KK2535 pKa = 11.21CPIVVGASDD2544 pKa = 3.92SGRR2547 pKa = 11.84VIPGVASDD2555 pKa = 4.13VYY2557 pKa = 11.42LLGKK2561 pKa = 8.25TLVFTLKK2568 pKa = 9.84TVFGSAGHH2576 pKa = 6.64CYY2578 pKa = 9.98DD2579 pKa = 3.64VNGIADD2585 pKa = 3.7GDD2587 pKa = 3.71KK2588 pKa = 11.16CLFNSACTNLEE2599 pKa = 3.86GLGGTRR2605 pKa = 11.84TYY2607 pKa = 10.42CYY2609 pKa = 10.48KK2610 pKa = 10.67PGLIDD2615 pKa = 3.86GAMTYY2620 pKa = 11.4GDD2622 pKa = 4.95LEE2624 pKa = 5.03HH2625 pKa = 7.46DD2626 pKa = 3.42SYY2628 pKa = 12.18YY2629 pKa = 10.83RR2630 pKa = 11.84LSGDD2634 pKa = 3.21NYY2636 pKa = 9.23VRR2638 pKa = 11.84LPYY2641 pKa = 10.64VVVQGLGFRR2650 pKa = 11.84AVRR2653 pKa = 11.84TQATTYY2659 pKa = 10.2CRR2661 pKa = 11.84VGEE2664 pKa = 4.42CVDD2667 pKa = 4.44SKK2669 pKa = 11.86AGMCFGADD2677 pKa = 2.4RR2678 pKa = 11.84WMVYY2682 pKa = 10.84SNDD2685 pKa = 3.28IGSDD2689 pKa = 4.05FICGSSLMDD2698 pKa = 4.11LLRR2701 pKa = 11.84NVLSVFNYY2709 pKa = 10.02NFSTMIMSGQVIFNCVLACVVVFGCYY2735 pKa = 8.36FVMKK2739 pKa = 9.62FRR2741 pKa = 11.84RR2742 pKa = 11.84VFGDD2746 pKa = 2.85MSLAVFTVCAAVVVNNLSYY2765 pKa = 10.62FISLNYY2771 pKa = 10.21VGMVVYY2777 pKa = 10.69SFLYY2781 pKa = 10.09FISTRR2786 pKa = 11.84GLKK2789 pKa = 10.27YY2790 pKa = 10.66CFIWDD2795 pKa = 3.39ISYY2798 pKa = 10.78VVAYY2802 pKa = 10.26CLLAPWWLLTWYY2814 pKa = 10.15VCAALIGLIPNLFKK2828 pKa = 11.25LKK2830 pKa = 10.75VSTTLFEE2837 pKa = 4.04GTKK2840 pKa = 10.36FVGTFDD2846 pKa = 3.62AASVGTFVIDD2856 pKa = 3.21ARR2858 pKa = 11.84SYY2860 pKa = 10.41EE2861 pKa = 3.97RR2862 pKa = 11.84LINSTSIEE2870 pKa = 4.16KK2871 pKa = 10.04IKK2873 pKa = 10.79QYY2875 pKa = 11.45ASTFNKK2881 pKa = 8.57YY2882 pKa = 9.98KK2883 pKa = 10.51YY2884 pKa = 10.42YY2885 pKa = 10.67SGSANEE2891 pKa = 3.67ADD2893 pKa = 3.96YY2894 pKa = 11.4RR2895 pKa = 11.84CACYY2899 pKa = 10.43AHH2901 pKa = 7.25LAKK2904 pKa = 10.79ALIDD2908 pKa = 4.13FSTTRR2913 pKa = 11.84QDD2915 pKa = 3.32TLYY2918 pKa = 10.06TPPTVSINSTLQAGLKK2934 pKa = 10.37KK2935 pKa = 9.41MAQPSGLVEE2944 pKa = 4.0PCVVRR2949 pKa = 11.84VSYY2952 pKa = 11.41GNTVLNGVWLDD2963 pKa = 3.71DD2964 pKa = 3.52KK2965 pKa = 10.97VYY2967 pKa = 10.67CPRR2970 pKa = 11.84HH2971 pKa = 5.28VLASDD2976 pKa = 3.33TTVTIDD2982 pKa = 3.22YY2983 pKa = 9.71DD2984 pKa = 3.56AAYY2987 pKa = 10.68HH2988 pKa = 6.77SMRR2991 pKa = 11.84LHH2993 pKa = 6.23NFSISKK2999 pKa = 10.64GNVFLGVVGAVMQGANLVITVSQANVNTPSYY3030 pKa = 11.12SFRR3033 pKa = 11.84TLKK3036 pKa = 10.76AGEE3039 pKa = 4.5CFNILACYY3047 pKa = 10.11DD3048 pKa = 3.48GTPAGVYY3055 pKa = 9.67GVNLRR3060 pKa = 11.84STHH3063 pKa = 5.43TIKK3066 pKa = 10.92GSFVNGACGSPGFVMNGHH3084 pKa = 5.88KK3085 pKa = 10.61VEE3087 pKa = 4.46FVYY3090 pKa = 9.2MHH3092 pKa = 6.42QIEE3095 pKa = 5.06LGNASHH3101 pKa = 6.73VGSDD3105 pKa = 3.36MFGNIYY3111 pKa = 10.62GGFDD3115 pKa = 3.94DD3116 pKa = 4.77QPSIQLEE3123 pKa = 4.57GVATLITEE3131 pKa = 4.02NVIAFLYY3138 pKa = 10.29AALINGEE3145 pKa = 4.26RR3146 pKa = 11.84WWCSNEE3152 pKa = 3.47RR3153 pKa = 11.84CTIDD3157 pKa = 5.55SFNEE3161 pKa = 3.48WALGNGFTNLVTGDD3175 pKa = 4.12GFSMLAAKK3183 pKa = 9.52TGIDD3187 pKa = 3.31VCQILSAIQRR3197 pKa = 11.84LATGLGGKK3205 pKa = 8.17TILGYY3210 pKa = 11.08ASLTDD3215 pKa = 4.03EE3216 pKa = 4.44YY3217 pKa = 10.55TLSEE3221 pKa = 4.71VVRR3224 pKa = 11.84QMYY3227 pKa = 9.37GVNIQSTKK3235 pKa = 9.94TSSALKK3241 pKa = 10.26NLLLMGFFFLLFWSEE3256 pKa = 3.63FFMYY3260 pKa = 9.35STILWINPGLITTFLGLFVMLSMLLASCIKK3290 pKa = 10.5HH3291 pKa = 6.35KK3292 pKa = 10.45MLFLQLFLLPSIIIAACYY3310 pKa = 9.13NFAWDD3315 pKa = 3.77MEE3317 pKa = 4.69VTRR3320 pKa = 11.84MLANQFDD3327 pKa = 4.36YY3328 pKa = 10.93HH3329 pKa = 8.44VSFLNMDD3336 pKa = 3.02IQGAINIIVCFIGISLHH3353 pKa = 5.59TYY3355 pKa = 10.32RR3356 pKa = 11.84FLDD3359 pKa = 3.59TQLRR3363 pKa = 11.84SYY3365 pKa = 8.95STYY3368 pKa = 9.78VLSMCTVLYY3377 pKa = 9.38TFYY3380 pKa = 10.92YY3381 pKa = 10.49GYY3383 pKa = 10.87DD3384 pKa = 3.71SLSLAIMLLGFGCRR3398 pKa = 11.84EE3399 pKa = 4.19WYY3401 pKa = 10.43VGTAAFRR3408 pKa = 11.84LAQFLVPYY3416 pKa = 9.77CPGLITFVGDD3426 pKa = 3.73IKK3428 pKa = 11.12AVLVLYY3434 pKa = 10.26LVFGFIATVYY3444 pKa = 10.56FGLLYY3449 pKa = 10.07WLNRR3453 pKa = 11.84VLKK3456 pKa = 9.09LTLGCYY3462 pKa = 9.51DD3463 pKa = 4.26FKK3465 pKa = 11.66VSAAEE3470 pKa = 3.89FKK3472 pKa = 11.22YY3473 pKa = 10.0MVANGYY3479 pKa = 6.08TAPRR3483 pKa = 11.84GPFDD3487 pKa = 4.28SVLLSLRR3494 pKa = 11.84LLGVGGHH3501 pKa = 5.29KK3502 pKa = 9.21TIKK3505 pKa = 10.42VSTVQSKK3512 pKa = 8.1LTDD3515 pKa = 4.02LKK3517 pKa = 10.76CANVVLLGCLTNMNIAANSRR3537 pKa = 11.84EE3538 pKa = 3.27WSYY3541 pKa = 11.71CVNLHH3546 pKa = 6.1NEE3548 pKa = 4.11INLTSDD3554 pKa = 3.45PEE3556 pKa = 3.98EE3557 pKa = 4.32ALEE3560 pKa = 4.5KK3561 pKa = 10.87LLALVAFFLSKK3572 pKa = 10.2QQNFGVDD3579 pKa = 3.55DD3580 pKa = 5.47LIDD3583 pKa = 3.57SFFEE3587 pKa = 3.95NRR3589 pKa = 11.84NVLQSVASAFANMPSFIAYY3608 pKa = 7.74EE3609 pKa = 4.14KK3610 pKa = 11.18ARR3612 pKa = 11.84MNYY3615 pKa = 9.47EE3616 pKa = 3.65DD3617 pKa = 5.93AIANDD3622 pKa = 3.89ASPAVIKK3629 pKa = 9.42QLKK3632 pKa = 9.37KK3633 pKa = 11.09AMNTAKK3639 pKa = 10.85GEE3641 pKa = 4.02FDD3643 pKa = 4.31HH3644 pKa = 7.02EE3645 pKa = 4.67SSVQKK3650 pKa = 10.45KK3651 pKa = 6.73IQRR3654 pKa = 11.84MADD3657 pKa = 2.89AAAAQMYY3664 pKa = 10.1KK3665 pKa = 10.49DD3666 pKa = 3.47ARR3668 pKa = 11.84AVDD3671 pKa = 3.89RR3672 pKa = 11.84KK3673 pKa = 10.36SKK3675 pKa = 10.48VVSAMHH3681 pKa = 6.14SLLFGMLRR3689 pKa = 11.84KK3690 pKa = 9.72LDD3692 pKa = 3.65MSSINQLMEE3701 pKa = 4.03LAKK3704 pKa = 10.74DD3705 pKa = 3.58GCIPMAIIPAAAATKK3720 pKa = 9.14LTVITPDD3727 pKa = 3.54LEE3729 pKa = 4.47SFSKK3733 pKa = 10.48IRR3735 pKa = 11.84VDD3737 pKa = 3.51NNIYY3741 pKa = 9.96YY3742 pKa = 10.57AGAAWSITDD3751 pKa = 3.73VKK3753 pKa = 11.0DD3754 pKa = 3.1ADD3756 pKa = 3.73GRR3758 pKa = 11.84VVILKK3763 pKa = 9.06EE3764 pKa = 4.04VNVDD3768 pKa = 3.38NKK3770 pKa = 11.02DD3771 pKa = 3.43ALVWPLHH3778 pKa = 5.29VNCEE3782 pKa = 4.03RR3783 pKa = 11.84VVKK3786 pKa = 10.09LQNNEE3791 pKa = 4.14IIPGKK3796 pKa = 9.86LKK3798 pKa = 10.31QRR3800 pKa = 11.84AVKK3803 pKa = 10.83AEE3805 pKa = 3.81GDD3807 pKa = 4.01GFSTDD3812 pKa = 4.42AKK3814 pKa = 10.78ALYY3817 pKa = 8.45NTEE3820 pKa = 4.27GGHH3823 pKa = 5.87HH3824 pKa = 6.38FVYY3827 pKa = 10.88ALIADD3832 pKa = 4.01KK3833 pKa = 10.87PDD3835 pKa = 3.31LKK3837 pKa = 10.29VVKK3840 pKa = 9.66WEE3842 pKa = 3.95FDD3844 pKa = 3.64GGCKK3848 pKa = 9.61TIEE3851 pKa = 4.37LEE3853 pKa = 4.37PPLKK3857 pKa = 10.34FAVEE3861 pKa = 4.26APTGVQIKK3869 pKa = 9.81YY3870 pKa = 10.38LYY3872 pKa = 9.6YY3873 pKa = 10.81VKK3875 pKa = 10.66NLNNLRR3881 pKa = 11.84RR3882 pKa = 11.84GATLGYY3888 pKa = 9.86IGATVRR3894 pKa = 11.84LQAGKK3899 pKa = 7.54QTEE3902 pKa = 4.35MACNSSLLTICAFAVDD3918 pKa = 3.77PAKK3921 pKa = 10.78AYY3923 pKa = 10.95VNAVKK3928 pKa = 10.48QGAKK3932 pKa = 10.04PVGNCIKK3939 pKa = 10.05MLANGSGSGQAITNGVEE3956 pKa = 3.6ASMNQDD3962 pKa = 2.92SYY3964 pKa = 12.01GGASVCIYY3972 pKa = 10.44CRR3974 pKa = 11.84AHH3976 pKa = 6.29VDD3978 pKa = 3.47HH3979 pKa = 7.41PAMDD3983 pKa = 4.55GACRR3987 pKa = 11.84FKK3989 pKa = 11.01GKK3991 pKa = 10.07YY3992 pKa = 6.94VQIPIGVNDD4001 pKa = 5.29PIGFCIEE4008 pKa = 4.03NEE4010 pKa = 4.09VCKK4013 pKa = 11.13VCGCWLNNGCSCDD4026 pKa = 3.55RR4027 pKa = 11.84SSVQSTDD4034 pKa = 2.9QAYY4037 pKa = 10.58LNRR4040 pKa = 11.84ARR4042 pKa = 11.84GSSAARR4048 pKa = 11.84LEE4050 pKa = 4.46PCNGTEE4056 pKa = 4.13PEE4058 pKa = 4.12HH4059 pKa = 6.56CVRR4062 pKa = 11.84AFDD4065 pKa = 4.75VYY4067 pKa = 11.22NKK4069 pKa = 10.43DD4070 pKa = 3.4VACIGKK4076 pKa = 8.86FLKK4079 pKa = 10.61VNCVRR4084 pKa = 11.84LKK4086 pKa = 11.11NLDD4089 pKa = 3.17KK4090 pKa = 11.04HH4091 pKa = 6.73DD4092 pKa = 3.83AFFVIKK4098 pKa = 10.2RR4099 pKa = 11.84CTKK4102 pKa = 10.13SVMEE4106 pKa = 5.15HH4107 pKa = 5.31EE4108 pKa = 3.75QSMYY4112 pKa = 11.36NKK4114 pKa = 10.34LSGSNALAVHH4124 pKa = 7.35DD4125 pKa = 4.41FFTWKK4130 pKa = 10.2DD4131 pKa = 3.29GRR4133 pKa = 11.84SIYY4136 pKa = 11.12GNVCRR4141 pKa = 11.84QDD4143 pKa = 3.33LSKK4146 pKa = 10.52YY4147 pKa = 9.2TMMDD4151 pKa = 3.14LCYY4154 pKa = 10.58ALRR4157 pKa = 11.84NFDD4160 pKa = 3.74EE4161 pKa = 4.91RR4162 pKa = 11.84NCEE4165 pKa = 3.97TLKK4168 pKa = 10.9EE4169 pKa = 4.04ILVLTGCCDD4178 pKa = 3.45QSYY4181 pKa = 10.67FDD4183 pKa = 3.77NKK4185 pKa = 8.39VWYY4188 pKa = 9.88DD4189 pKa = 3.49PVEE4192 pKa = 4.44NEE4194 pKa = 4.1DD4195 pKa = 3.4LHH4197 pKa = 8.38RR4198 pKa = 11.84VYY4200 pKa = 11.1ALLGQRR4206 pKa = 11.84VANAMLKK4213 pKa = 10.04CVKK4216 pKa = 10.31LCDD4219 pKa = 3.56EE4220 pKa = 4.69MVTKK4224 pKa = 10.64GVVGVLTLDD4233 pKa = 3.78NQDD4236 pKa = 3.91LNGNFYY4242 pKa = 11.18DD4243 pKa = 4.61FGDD4246 pKa = 4.23FVDD4249 pKa = 4.22VMPGMGIPCCTSYY4262 pKa = 11.13YY4263 pKa = 10.41SYY4265 pKa = 10.79MMPIMTMTNCLACEE4279 pKa = 4.35CFMKK4283 pKa = 10.63SDD4285 pKa = 3.47IFGSDD4290 pKa = 3.01FKK4292 pKa = 10.98TYY4294 pKa = 11.19DD4295 pKa = 3.43LLEE4298 pKa = 4.42YY4299 pKa = 10.7DD4300 pKa = 3.97FTDD4303 pKa = 3.22HH4304 pKa = 7.22KK4305 pKa = 11.54VKK4307 pKa = 10.93LFDD4310 pKa = 4.99KK4311 pKa = 10.48YY4312 pKa = 10.77FKK4314 pKa = 11.05YY4315 pKa = 9.82WGQDD4319 pKa = 3.22YY4320 pKa = 10.88HH4321 pKa = 7.0PNCSDD4326 pKa = 4.9CYY4328 pKa = 11.48DD4329 pKa = 4.02DD4330 pKa = 5.18MCLLHH4335 pKa = 6.84CSNFNTLFSTTIPNTAFGPLCRR4357 pKa = 11.84KK4358 pKa = 9.7VFVDD4362 pKa = 4.74GVPLIATAGYY4372 pKa = 9.97HH4373 pKa = 6.02FKK4375 pKa = 10.85QLGLVWNKK4383 pKa = 10.65DD4384 pKa = 3.29INTHH4388 pKa = 5.47NSRR4391 pKa = 11.84LSMTDD4396 pKa = 3.17LLQFVTDD4403 pKa = 4.42PGLLIASSPALVDD4416 pKa = 3.22QRR4418 pKa = 11.84TVCFSIAALSTGITHH4433 pKa = 5.12QTVKK4437 pKa = 10.27PGHH4440 pKa = 5.83FNKK4443 pKa = 10.11EE4444 pKa = 3.8FYY4446 pKa = 10.34DD4447 pKa = 3.81YY4448 pKa = 11.32LLSQGFFDD4456 pKa = 4.58EE4457 pKa = 5.5GSEE4460 pKa = 4.03LTLKK4464 pKa = 10.76HH4465 pKa = 6.43FFFAQKK4471 pKa = 10.24GDD4473 pKa = 3.54AAVADD4478 pKa = 4.27FDD4480 pKa = 4.51YY4481 pKa = 11.37YY4482 pKa = 10.98RR4483 pKa = 11.84YY4484 pKa = 10.49NKK4486 pKa = 8.5PTMLDD4491 pKa = 2.44ICMARR4496 pKa = 11.84FTYY4499 pKa = 10.29KK4500 pKa = 9.81VVQRR4504 pKa = 11.84YY4505 pKa = 7.45FEE4507 pKa = 4.94CYY4509 pKa = 10.14DD4510 pKa = 3.61GGCITARR4517 pKa = 11.84EE4518 pKa = 4.1VVVTNLDD4525 pKa = 3.15KK4526 pKa = 11.38SAGYY4530 pKa = 9.09PLNRR4534 pKa = 11.84FGKK4537 pKa = 10.17ARR4539 pKa = 11.84LFYY4542 pKa = 9.72EE4543 pKa = 4.14TFSYY4547 pKa = 11.01EE4548 pKa = 4.11EE4549 pKa = 3.74QDD4551 pKa = 3.27ALYY4554 pKa = 11.36AMTKK4558 pKa = 10.42RR4559 pKa = 11.84NILPTMTQLNLKK4571 pKa = 10.1YY4572 pKa = 10.42SISGKK4577 pKa = 10.08ARR4579 pKa = 11.84ARR4581 pKa = 11.84TVGGVSLLATMTTRR4595 pKa = 11.84QFHH4598 pKa = 5.39QKK4600 pKa = 9.48HH4601 pKa = 5.9LKK4603 pKa = 10.62SIVNTRR4609 pKa = 11.84NAPVVIGTTKK4619 pKa = 10.65FYY4621 pKa = 11.25GGWDD4625 pKa = 3.44NMLKK4629 pKa = 10.69NLMNDD4634 pKa = 3.11VDD4636 pKa = 4.42NGALMGWDD4644 pKa = 3.58YY4645 pKa = 10.79PKK4647 pKa = 10.32CDD4649 pKa = 3.03RR4650 pKa = 11.84AMPSMIRR4657 pKa = 11.84MLAAMVLGSKK4667 pKa = 10.14HH4668 pKa = 4.82VTCCTDD4674 pKa = 2.46SDD4676 pKa = 3.67RR4677 pKa = 11.84FYY4679 pKa = 11.32RR4680 pKa = 11.84LSNEE4684 pKa = 3.81LAQVLNEE4691 pKa = 4.15VVHH4694 pKa = 6.51SNGGFYY4700 pKa = 10.64VKK4702 pKa = 10.48PGGTTSGDD4710 pKa = 3.05ATTAYY4715 pKa = 10.31ANSVFNIFQAVSSNINRR4732 pKa = 11.84LLSVDD4737 pKa = 4.24SNVCNNLYY4745 pKa = 10.22VKK4747 pKa = 10.45KK4748 pKa = 9.44LQRR4751 pKa = 11.84SIYY4754 pKa = 8.53DD4755 pKa = 3.06NCYY4758 pKa = 9.91RR4759 pKa = 11.84SSAVDD4764 pKa = 3.59DD4765 pKa = 4.03NVVTEE4770 pKa = 4.82FYY4772 pKa = 11.15NYY4774 pKa = 9.93LKK4776 pKa = 10.61KK4777 pKa = 10.15HH4778 pKa = 5.97FSMMILSDD4786 pKa = 4.35DD4787 pKa = 4.63GVVCYY4792 pKa = 10.56NKK4794 pKa = 10.19EE4795 pKa = 3.96YY4796 pKa = 10.9AALGYY4801 pKa = 10.73VGDD4804 pKa = 4.12ISAFKK4809 pKa = 9.74ATLYY4813 pKa = 8.95YY4814 pKa = 10.1QNNVFMSTAKK4824 pKa = 10.3CWVEE4828 pKa = 3.6EE4829 pKa = 4.06DD4830 pKa = 4.31LSVGPHH4836 pKa = 6.03EE4837 pKa = 5.13FCSQHH4842 pKa = 4.88TMQIVDD4848 pKa = 3.68SDD4850 pKa = 4.03GEE4852 pKa = 4.52YY4853 pKa = 10.72YY4854 pKa = 10.7LPYY4857 pKa = 9.27PDD4859 pKa = 4.57PSRR4862 pKa = 11.84ILSAGVFVDD4871 pKa = 6.12DD4872 pKa = 4.96IVKK4875 pKa = 9.13TDD4877 pKa = 3.68PVILLEE4883 pKa = 4.71RR4884 pKa = 11.84YY4885 pKa = 9.28VSLAIDD4891 pKa = 4.72AYY4893 pKa = 9.89PLSKK4897 pKa = 10.59HH4898 pKa = 5.86PNRR4901 pKa = 11.84EE4902 pKa = 3.84YY4903 pKa = 10.96RR4904 pKa = 11.84KK4905 pKa = 9.51VFYY4908 pKa = 11.14VLLDD4912 pKa = 3.74WVKK4915 pKa = 10.59HH4916 pKa = 4.63LHH4918 pKa = 5.27NTLNQGILEE4927 pKa = 4.44TFSVTLLDD4935 pKa = 4.48DD4936 pKa = 3.98VQSKK4940 pKa = 9.66FWDD4943 pKa = 3.52EE4944 pKa = 3.4AFYY4947 pKa = 11.13AGMYY4951 pKa = 7.9EE4952 pKa = 4.37KK4953 pKa = 10.23STVLQAAGMCIVCGSQTVLRR4973 pKa = 11.84CGDD4976 pKa = 3.76CLRR4979 pKa = 11.84RR4980 pKa = 11.84PLLCTKK4986 pKa = 10.15CAYY4989 pKa = 10.32DD4990 pKa = 3.48HH4991 pKa = 6.88VVGTTHH4997 pKa = 7.87RR4998 pKa = 11.84FILSITPYY5006 pKa = 9.38VCNTSGCNVNDD5017 pKa = 3.39VTKK5020 pKa = 11.0LFLGGLNYY5028 pKa = 10.26YY5029 pKa = 10.35CHH5031 pKa = 6.79DD5032 pKa = 4.81HH5033 pKa = 6.88KK5034 pKa = 10.96PQLSFPLCANGNIFGLYY5051 pKa = 9.41KK5052 pKa = 10.6NSAVGSLDD5060 pKa = 3.18VEE5062 pKa = 4.69VFNKK5066 pKa = 10.39LAMSDD5071 pKa = 3.16WSDD5074 pKa = 3.43VSDD5077 pKa = 3.67YY5078 pKa = 11.61KK5079 pKa = 10.95LANDD5083 pKa = 3.5VKK5085 pKa = 10.69EE5086 pKa = 4.19SLRR5089 pKa = 11.84LFAAEE5094 pKa = 4.04TIKK5097 pKa = 10.9ARR5099 pKa = 11.84EE5100 pKa = 4.01EE5101 pKa = 4.42SVKK5104 pKa = 10.67SSYY5107 pKa = 11.62ACATLKK5113 pKa = 10.69EE5114 pKa = 4.27IIGPKK5119 pKa = 9.78EE5120 pKa = 4.51LLLQWEE5126 pKa = 4.62VGKK5129 pKa = 10.55ARR5131 pKa = 11.84PPLNRR5136 pKa = 11.84NSVFTCFQISKK5147 pKa = 9.56DD5148 pKa = 3.97SKK5150 pKa = 7.62WQVGEE5155 pKa = 4.12FTFEE5159 pKa = 3.96KK5160 pKa = 10.35LDD5162 pKa = 3.77YY5163 pKa = 11.34GSDD5166 pKa = 3.72TVCYY5170 pKa = 9.69KK5171 pKa = 11.25SNVTAKK5177 pKa = 9.87LVPGMIFVLTSHH5189 pKa = 6.58NVLSLKK5195 pKa = 10.78APTIANQEE5203 pKa = 4.16RR5204 pKa = 11.84YY5205 pKa = 7.51STIYY5209 pKa = 10.68KK5210 pKa = 10.1LYY5212 pKa = 10.02PSLNVDD5218 pKa = 3.64DD5219 pKa = 5.84AYY5221 pKa = 11.69SSLVPYY5227 pKa = 9.55YY5228 pKa = 10.69QLIGKK5233 pKa = 8.02QKK5235 pKa = 8.31ITTIQGPPGSGKK5247 pKa = 7.24SHH5249 pKa = 6.22CVIGLGLYY5257 pKa = 9.45YY5258 pKa = 9.96PSARR5262 pKa = 11.84IVFAACSHH5270 pKa = 6.34AAVDD5274 pKa = 4.25SLCHH5278 pKa = 6.08KK5279 pKa = 9.79AAKK5282 pKa = 9.89AYY5284 pKa = 10.75SVDD5287 pKa = 3.29RR5288 pKa = 11.84CSRR5291 pKa = 11.84IIPARR5296 pKa = 11.84ARR5298 pKa = 11.84VEE5300 pKa = 4.34CYY5302 pKa = 10.61SGFKK5306 pKa = 10.12PNNTSAQYY5314 pKa = 10.65IFSTVNALPEE5324 pKa = 4.03VNADD5328 pKa = 2.73IVVIDD5333 pKa = 4.4EE5334 pKa = 4.41VSMCTNYY5341 pKa = 10.78DD5342 pKa = 3.48LSIVNARR5349 pKa = 11.84VAYY5352 pKa = 9.36KK5353 pKa = 10.35HH5354 pKa = 5.78IVYY5357 pKa = 10.63VGDD5360 pKa = 3.88PQQLPAPRR5368 pKa = 11.84TMITRR5373 pKa = 11.84GVLQPEE5379 pKa = 4.85DD5380 pKa = 4.09YY5381 pKa = 11.15NVVTQRR5387 pKa = 11.84MCGVGPDD5394 pKa = 3.71VFLHH5398 pKa = 5.46KK5399 pKa = 10.27CYY5401 pKa = 10.2RR5402 pKa = 11.84CPAEE5406 pKa = 4.0VVNTVSEE5413 pKa = 4.13LVYY5416 pKa = 10.43EE5417 pKa = 4.48NKK5419 pKa = 10.06FKK5421 pKa = 10.55PVKK5424 pKa = 10.2DD5425 pKa = 3.58HH5426 pKa = 7.27SKK5428 pKa = 9.65QCFKK5432 pKa = 10.79MFVKK5436 pKa = 10.87GNVQIDD5442 pKa = 3.45NGSSVNKK5449 pKa = 9.97KK5450 pKa = 7.99QLEE5453 pKa = 4.32VVKK5456 pKa = 10.92AFIAKK5461 pKa = 8.93NPKK5464 pKa = 7.86WSRR5467 pKa = 11.84AVFISPYY5474 pKa = 10.18NSQNYY5479 pKa = 7.8VAGRR5483 pKa = 11.84MLGLQTQTVDD5493 pKa = 3.0SAQGSEE5499 pKa = 4.16YY5500 pKa = 10.73DD5501 pKa = 3.76YY5502 pKa = 11.43VIYY5505 pKa = 9.01TQTSDD5510 pKa = 2.78TSHH5513 pKa = 6.95ALNVNRR5519 pKa = 11.84FNVAITRR5526 pKa = 11.84TKK5528 pKa = 10.46IGILCIMCDD5537 pKa = 3.13KK5538 pKa = 11.07ALYY5541 pKa = 7.75DD5542 pKa = 3.43TLKK5545 pKa = 10.52FFEE5548 pKa = 4.94ISQSDD5553 pKa = 3.89LQSTIGGCGLFKK5565 pKa = 10.69DD5566 pKa = 4.98CYY5568 pKa = 10.96KK5569 pKa = 10.43FDD5571 pKa = 3.7QDD5573 pKa = 5.19LPPAHH5578 pKa = 6.82ATTYY5582 pKa = 8.97MALSDD5587 pKa = 3.75KK5588 pKa = 10.84FKK5590 pKa = 10.45TDD5592 pKa = 2.77KK5593 pKa = 10.76EE5594 pKa = 4.03LAVNIGHH5601 pKa = 6.33SDD5603 pKa = 2.91VRR5605 pKa = 11.84YY5606 pKa = 7.88EE5607 pKa = 4.08HH5608 pKa = 6.6VVSFMGFRR5616 pKa = 11.84FDD5618 pKa = 3.5MNIPNFHH5625 pKa = 6.96SLFCTRR5631 pKa = 11.84DD5632 pKa = 3.02FAMRR5636 pKa = 11.84NVRR5639 pKa = 11.84GWIGMDD5645 pKa = 3.0VEE5647 pKa = 4.73GAHH5650 pKa = 6.08VCGDD5654 pKa = 3.81NIGTNVPLQVGFSNGVDD5671 pKa = 3.44FVVQPEE5677 pKa = 4.79GCVVTNDD5684 pKa = 3.41GNIVKK5689 pKa = 9.3PVKK5692 pKa = 10.37ARR5694 pKa = 11.84APPGEE5699 pKa = 4.36QFTHH5703 pKa = 6.77LVPLMRR5709 pKa = 11.84KK5710 pKa = 6.95GQPWYY5715 pKa = 9.15VVRR5718 pKa = 11.84RR5719 pKa = 11.84RR5720 pKa = 11.84IVQMVCDD5727 pKa = 4.44CLNGLSDD5734 pKa = 3.87VVIFVLWAGGLEE5746 pKa = 4.41LTTMKK5751 pKa = 10.51YY5752 pKa = 9.43FVKK5755 pKa = 10.22IGPSQHH5761 pKa = 6.63CDD5763 pKa = 2.82CGKK5766 pKa = 10.09EE5767 pKa = 3.92ARR5769 pKa = 11.84CYY5771 pKa = 10.89NSATHH5776 pKa = 7.29AYY5778 pKa = 9.03YY5779 pKa = 10.86CLAHH5783 pKa = 7.2ALGCDD5788 pKa = 3.44YY5789 pKa = 10.82LYY5791 pKa = 11.25NPFVIDD5797 pKa = 3.62IQQWGYY5803 pKa = 9.09TGSLSSNHH5811 pKa = 6.58HH5812 pKa = 5.56EE5813 pKa = 4.17VCNVHH5818 pKa = 6.69RR5819 pKa = 11.84NEE5821 pKa = 4.43HH5822 pKa = 5.65VASGDD5827 pKa = 3.82AIMTRR5832 pKa = 11.84CLAIHH5837 pKa = 7.01DD5838 pKa = 4.3CFVKK5842 pKa = 10.79NVDD5845 pKa = 3.01WSITYY5850 pKa = 8.75PFIANEE5856 pKa = 3.72NAINKK5861 pKa = 9.5SGRR5864 pKa = 11.84IVQSHH5869 pKa = 5.62IMKK5872 pKa = 10.4AALKK5876 pKa = 9.82VYY5878 pKa = 10.01NPKK5881 pKa = 10.27AVHH5884 pKa = 7.49DD5885 pKa = 3.88IGNPKK5890 pKa = 10.29GIRR5893 pKa = 11.84CAVTNVPWYY5902 pKa = 10.41CYY5904 pKa = 10.29DD5905 pKa = 3.5KK5906 pKa = 11.33QPINSNVKK5914 pKa = 8.55MLEE5917 pKa = 3.91YY5918 pKa = 10.58DD5919 pKa = 3.87YY5920 pKa = 8.12MTHH5923 pKa = 5.91GQLDD5927 pKa = 4.28GMCLFWNCNVDD5938 pKa = 3.51MYY5940 pKa = 11.17PEE5942 pKa = 4.07FSIVCRR5948 pKa = 11.84FDD5950 pKa = 3.23TRR5952 pKa = 11.84CRR5954 pKa = 11.84SHH5956 pKa = 7.4LSLEE5960 pKa = 4.41GVNGGALYY5968 pKa = 10.88VNNHH5972 pKa = 6.09AFHH5975 pKa = 6.51TPAYY5979 pKa = 9.66DD5980 pKa = 3.13KK5981 pKa = 11.2RR5982 pKa = 11.84AFAKK5986 pKa = 9.81LKK5988 pKa = 9.78PMPFFFYY5995 pKa = 10.56DD5996 pKa = 3.93DD5997 pKa = 4.48GEE5999 pKa = 5.33CDD6001 pKa = 3.42VSQGQINYY6009 pKa = 8.74VPLRR6013 pKa = 11.84ATVCITKK6020 pKa = 10.24CNIGGAVCKK6029 pKa = 10.07RR6030 pKa = 11.84HH6031 pKa = 5.45ASLYY6035 pKa = 8.29RR6036 pKa = 11.84AYY6038 pKa = 10.82VEE6040 pKa = 4.61AYY6042 pKa = 8.12NTFTQNGFNIWCPNSFDD6059 pKa = 5.64VYY6061 pKa = 11.18NLWQTLVDD6069 pKa = 3.79TNLQGLEE6076 pKa = 3.92NIAFNVVKK6084 pKa = 10.4KK6085 pKa = 10.66GSFVGEE6091 pKa = 4.06PGEE6094 pKa = 4.26LPVAVVNDD6102 pKa = 3.89RR6103 pKa = 11.84VSVRR6107 pKa = 11.84DD6108 pKa = 3.73GVSDD6112 pKa = 3.71NVIFVNKK6119 pKa = 9.45TSLPTNVAFEE6129 pKa = 4.87LYY6131 pKa = 9.98AKK6133 pKa = 10.15RR6134 pKa = 11.84KK6135 pKa = 9.47IGLTPPLTILKK6146 pKa = 10.36NMGVVATHH6154 pKa = 6.78GFVLWDD6160 pKa = 3.57YY6161 pKa = 11.5DD6162 pKa = 3.51ADD6164 pKa = 4.23RR6165 pKa = 11.84PFSNFTKK6172 pKa = 10.35SVCKK6176 pKa = 9.93YY6177 pKa = 8.68TDD6179 pKa = 3.54FDD6181 pKa = 4.94EE6182 pKa = 5.6DD6183 pKa = 4.01VCTCFDD6189 pKa = 3.85NSIQGAFEE6197 pKa = 4.83RR6198 pKa = 11.84FTLCKK6203 pKa = 10.45NGVLISNVAIKK6214 pKa = 10.25KK6215 pKa = 9.9LRR6217 pKa = 11.84GIRR6220 pKa = 11.84LNFGYY6225 pKa = 10.67LNGVAVSNVTEE6236 pKa = 4.41GEE6238 pKa = 4.19TTKK6241 pKa = 10.88PVDD6244 pKa = 2.81WYY6246 pKa = 10.22IYY6248 pKa = 7.86QRR6250 pKa = 11.84KK6251 pKa = 9.52DD6252 pKa = 2.94GSFVEE6257 pKa = 4.79PVDD6260 pKa = 4.32GYY6262 pKa = 11.49YY6263 pKa = 10.54SQGRR6267 pKa = 11.84NASTFLPRR6275 pKa = 11.84SQMEE6279 pKa = 4.03KK6280 pKa = 11.02DD6281 pKa = 3.61FLEE6284 pKa = 4.91LDD6286 pKa = 3.2TGLFISKK6293 pKa = 10.5YY6294 pKa = 9.86GLEE6297 pKa = 4.49DD6298 pKa = 4.07FNFEE6302 pKa = 4.48HH6303 pKa = 6.85IVYY6306 pKa = 10.43GDD6308 pKa = 3.47VSKK6311 pKa = 8.23TTLGGLHH6318 pKa = 6.71LLISQVRR6325 pKa = 11.84LARR6328 pKa = 11.84IGVLKK6333 pKa = 11.18VEE6335 pKa = 4.88DD6336 pKa = 4.57FVDD6339 pKa = 3.83SADD6342 pKa = 4.1DD6343 pKa = 3.81TLHH6346 pKa = 6.14SCSVTYY6352 pKa = 11.09ANDD6355 pKa = 3.77PSSKK6359 pKa = 10.39SVCTYY6364 pKa = 10.51MDD6366 pKa = 3.42ILLDD6370 pKa = 3.99DD6371 pKa = 4.38FVVILKK6377 pKa = 10.29NLDD6380 pKa = 3.38LSVTSKK6386 pKa = 10.11VHH6388 pKa = 7.28DD6389 pKa = 4.35VIVDD6393 pKa = 3.8CKK6395 pKa = 10.06AWRR6398 pKa = 11.84WMLWCKK6404 pKa = 10.34DD6405 pKa = 3.45SKK6407 pKa = 11.39VATFYY6412 pKa = 10.7PQLQSAEE6419 pKa = 4.3WKK6421 pKa = 9.76CGYY6424 pKa = 10.77SMPSLYY6430 pKa = 10.32KK6431 pKa = 9.6IQNMCMDD6438 pKa = 4.28ACNLYY6443 pKa = 10.9NYY6445 pKa = 8.27GASIKK6450 pKa = 10.64LPDD6453 pKa = 4.59GIMFNVVKK6461 pKa = 9.14YY6462 pKa = 8.18TQLCQFLNTTTMCVPHH6478 pKa = 6.47NMRR6481 pKa = 11.84VLHH6484 pKa = 6.62LGAGSDD6490 pKa = 3.36KK6491 pKa = 10.9GVAPGTAVLRR6501 pKa = 11.84RR6502 pKa = 11.84WLPDD6506 pKa = 3.25DD6507 pKa = 5.8AIIVDD6512 pKa = 4.11NDD6514 pKa = 3.25VNDD6517 pKa = 4.07YY6518 pKa = 11.37VSDD6521 pKa = 3.9ADD6523 pKa = 4.32FSITGDD6529 pKa = 3.6CTHH6532 pKa = 6.71VYY6534 pKa = 10.66VEE6536 pKa = 5.18DD6537 pKa = 4.49KK6538 pKa = 10.93FDD6540 pKa = 4.52LLISDD6545 pKa = 4.59MYY6547 pKa = 10.91DD6548 pKa = 3.16GKK6550 pKa = 10.68IKK6552 pKa = 10.82SIDD6555 pKa = 3.48GDD6557 pKa = 3.88NVSKK6561 pKa = 11.12DD6562 pKa = 3.28GFFTYY6567 pKa = 10.31INGFIRR6573 pKa = 11.84EE6574 pKa = 3.97KK6575 pKa = 10.58LALGGAMAVKK6585 pKa = 8.83ITEE6588 pKa = 4.2YY6589 pKa = 10.56SWNKK6593 pKa = 8.73QLYY6596 pKa = 9.28EE6597 pKa = 3.74IAQKK6601 pKa = 10.67FEE6603 pKa = 3.93YY6604 pKa = 7.5WTLFCTSVNTSSSEE6618 pKa = 3.7AFLIGINYY6626 pKa = 10.15LGDD6629 pKa = 3.8FSSTNVIDD6637 pKa = 4.28GNVMHH6642 pKa = 7.34ANYY6645 pKa = 9.97IFWRR6649 pKa = 11.84NSTIMTMSYY6658 pKa = 11.18NSVLDD6663 pKa = 3.84LSKK6666 pKa = 10.65FRR6668 pKa = 11.84CKK6670 pKa = 10.39HH6671 pKa = 5.06KK6672 pKa = 10.11ATVIITLKK6680 pKa = 11.04DD6681 pKa = 3.27KK6682 pKa = 11.03DD6683 pKa = 3.66ITDD6686 pKa = 3.73MVLGLIKK6693 pKa = 10.52NGKK6696 pKa = 9.05LLIRR6700 pKa = 11.84NSQKK6704 pKa = 10.83LLNFSNHH6711 pKa = 5.64LVTTKK6716 pKa = 10.92

Molecular weight:
748.53 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0U1WJW4|A0A0U1WJW4_9ALPC Spike glycoprotein OS=BtRf-AlphaCoV/YN2012 OX=1503293 PE=4 SV=1
MM1 pKa = 7.27ATVNWGDD8 pKa = 3.39AVEE11 pKa = 4.27AEE13 pKa = 4.23SRR15 pKa = 11.84GRR17 pKa = 11.84KK18 pKa = 8.48PRR20 pKa = 11.84IPLSLFAPLRR30 pKa = 11.84VTSDD34 pKa = 3.03KK35 pKa = 11.34NFWNVMPRR43 pKa = 11.84NGVPTGKK50 pKa = 10.51GNSEE54 pKa = 3.8QQIGYY59 pKa = 8.81WVEE62 pKa = 3.8QKK64 pKa = 9.42RR65 pKa = 11.84WRR67 pKa = 11.84MQKK70 pKa = 9.23GQRR73 pKa = 11.84KK74 pKa = 8.2DD75 pKa = 3.49QPSNWHH81 pKa = 6.59FYY83 pKa = 10.41YY84 pKa = 10.82LGTGPHH90 pKa = 6.63AEE92 pKa = 3.77APFRR96 pKa = 11.84KK97 pKa = 9.58RR98 pKa = 11.84IQGVHH103 pKa = 4.78WVAIDD108 pKa = 3.79GAKK111 pKa = 9.85TNPTGLGVRR120 pKa = 11.84NRR122 pKa = 11.84NRR124 pKa = 11.84EE125 pKa = 4.04PITPQFGFQLPSDD138 pKa = 3.86LMIVEE143 pKa = 4.41STSRR147 pKa = 11.84SASRR151 pKa = 11.84SQSRR155 pKa = 11.84SRR157 pKa = 11.84NQSQSRR163 pKa = 11.84SGQQTPRR170 pKa = 11.84FQQQSQSVDD179 pKa = 2.37IVAAVKK185 pKa = 10.09QALADD190 pKa = 4.03LGISGTQNKK199 pKa = 8.24PQSGRR204 pKa = 11.84NTPKK208 pKa = 9.57QKK210 pKa = 10.6SRR212 pKa = 11.84AVSPAPSPKK221 pKa = 8.49QTRR224 pKa = 11.84KK225 pKa = 10.21QMDD228 pKa = 3.26KK229 pKa = 10.87PEE231 pKa = 4.2WKK233 pKa = 9.88RR234 pKa = 11.84VPNSDD239 pKa = 3.91EE240 pKa = 4.3DD241 pKa = 3.85VKK243 pKa = 11.38KK244 pKa = 10.94CFGPRR249 pKa = 11.84SVARR253 pKa = 11.84NFGDD257 pKa = 3.94SDD259 pKa = 4.85LIQNGVEE266 pKa = 3.97AKK268 pKa = 10.16HH269 pKa = 5.98FPTIAEE275 pKa = 4.36LLPTQAAIAFGSEE288 pKa = 3.59ITTKK292 pKa = 10.34EE293 pKa = 3.54AGDD296 pKa = 3.74FVEE299 pKa = 4.34ITYY302 pKa = 9.93HH303 pKa = 6.53YY304 pKa = 10.28VMKK307 pKa = 10.71VPKK310 pKa = 9.18TDD312 pKa = 3.12KK313 pKa = 10.45NLPRR317 pKa = 11.84FLEE320 pKa = 4.12QVSAYY325 pKa = 10.09SKK327 pKa = 9.4PSQIRR332 pKa = 11.84RR333 pKa = 11.84SQSQQDD339 pKa = 4.01LNVDD343 pKa = 3.55ALVFTPAPPTTPTVQSPSFLEE364 pKa = 4.41EE365 pKa = 3.7EE366 pKa = 4.38VEE368 pKa = 4.29MVDD371 pKa = 5.13EE372 pKa = 5.29IINN375 pKa = 3.53

Molecular weight:
42.05 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

8755

75

6716

1459.2

162.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.636 ± 0.259

3.324 ± 0.79

5.848 ± 1.175

3.701 ± 0.268

5.837 ± 0.349

6.75 ± 0.463

1.999 ± 0.288

5.62 ± 0.624

5.688 ± 0.818

8.704 ± 0.692

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.159 ± 0.167

5.802 ± 0.269

3.404 ± 0.899

2.935 ± 0.994

3.529 ± 0.68

7.127 ± 0.502

5.962 ± 0.511

9.16 ± 0.686

1.325 ± 0.366

4.489 ± 0.485

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski