Mus musculus polyomavirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Betapolyomavirus

Average proteome isoelectric point is 5.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A2IBB5|A2IBB5_9POLY Minor capsid protein OS=Mus musculus polyomavirus 2 OX=1891770 GN=VP3 PE=3 SV=1
MM1 pKa = 7.63APTVKK6 pKa = 9.86KK7 pKa = 10.62RR8 pKa = 11.84SVAKK12 pKa = 9.86PRR14 pKa = 11.84PKK16 pKa = 9.99PSEE19 pKa = 4.01VPKK22 pKa = 10.63LIVVGGIQVLDD33 pKa = 3.72VRR35 pKa = 11.84TGPDD39 pKa = 3.32SITQIEE45 pKa = 4.54AFLNPRR51 pKa = 11.84MGQPVDD57 pKa = 3.17SDD59 pKa = 4.17FYY61 pKa = 11.45GFSDD65 pKa = 4.82NITVSADD72 pKa = 3.22YY73 pKa = 8.99TQDD76 pKa = 2.91MPRR79 pKa = 11.84IKK81 pKa = 10.19EE82 pKa = 4.05LPCYY86 pKa = 10.78SMAKK90 pKa = 9.84ISLPMLNEE98 pKa = 4.64DD99 pKa = 3.95MTCDD103 pKa = 3.74TILMWEE109 pKa = 4.8AISCKK114 pKa = 9.66TEE116 pKa = 3.86VVGVSSLTNCHH127 pKa = 5.87SAVKK131 pKa = 10.12RR132 pKa = 11.84LYY134 pKa = 10.45DD135 pKa = 3.48NEE137 pKa = 4.36GAGFPVQGLNFHH149 pKa = 6.83FFSVGGEE156 pKa = 3.94ALDD159 pKa = 3.56LQMVVEE165 pKa = 5.05NYY167 pKa = 9.11RR168 pKa = 11.84CKK170 pKa = 10.91YY171 pKa = 7.66PAGVAALQAAPKK183 pKa = 9.3AAQVLDD189 pKa = 4.09PKK191 pKa = 11.02LKK193 pKa = 10.87AKK195 pKa = 9.1LTADD199 pKa = 3.18GKK201 pKa = 11.03FPIEE205 pKa = 4.08AWSPDD210 pKa = 3.33PAKK213 pKa = 10.98NEE215 pKa = 3.57NTRR218 pKa = 11.84YY219 pKa = 9.81FGTYY223 pKa = 8.95TGGLQTPPVLQITNTTTTILLNEE246 pKa = 4.48NGVGPLCKK254 pKa = 10.17GDD256 pKa = 3.96GLYY259 pKa = 10.57LASADD264 pKa = 2.93IVGFRR269 pKa = 11.84TQQNNKK275 pKa = 5.22MHH277 pKa = 6.34FRR279 pKa = 11.84GLPRR283 pKa = 11.84YY284 pKa = 9.57FSITLRR290 pKa = 11.84KK291 pKa = 9.53RR292 pKa = 11.84LVKK295 pKa = 10.54NPYY298 pKa = 9.07PVSSLLNTLFSEE310 pKa = 4.5MMPQIDD316 pKa = 4.07GQKK319 pKa = 9.32MEE321 pKa = 5.04GEE323 pKa = 4.23DD324 pKa = 3.56AQVEE328 pKa = 4.36EE329 pKa = 4.05VRR331 pKa = 11.84IYY333 pKa = 11.17DD334 pKa = 3.6GVEE337 pKa = 3.91RR338 pKa = 11.84LPGDD342 pKa = 3.64PDD344 pKa = 3.65MIRR347 pKa = 11.84YY348 pKa = 8.92RR349 pKa = 11.84DD350 pKa = 3.45KK351 pKa = 10.71FGQEE355 pKa = 3.53VTVPDD360 pKa = 3.82IPSNEE365 pKa = 3.83AA366 pKa = 2.98

Molecular weight: 40.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A2IBB2|A2IBB2_9POLY Small t antigen OS=Mus musculus polyomavirus 2 OX=1891770 GN=tAg PE=4 SV=1
MM1 pKa = 7.81DD2 pKa = 4.18HH3 pKa = 6.6QLTRR7 pKa = 11.84EE8 pKa = 3.73EE9 pKa = 4.49SQRR12 pKa = 11.84LMHH15 pKa = 6.93LLKK18 pKa = 10.89LPMEE22 pKa = 4.45QYY24 pKa = 11.84GNFPLMRR31 pKa = 11.84KK32 pKa = 9.54AFLRR36 pKa = 11.84ACKK39 pKa = 9.54IVHH42 pKa = 6.61PDD44 pKa = 3.02KK45 pKa = 11.35GGSDD49 pKa = 3.77EE50 pKa = 5.34LSQEE54 pKa = 4.36LISLYY59 pKa = 10.33RR60 pKa = 11.84RR61 pKa = 11.84LEE63 pKa = 3.8EE64 pKa = 4.71SLPCLSTQDD73 pKa = 4.68FIEE76 pKa = 4.14TDD78 pKa = 2.93ILQIPSYY85 pKa = 9.06GTPEE89 pKa = 3.44WDD91 pKa = 3.42EE92 pKa = 3.5WWKK95 pKa = 10.81EE96 pKa = 3.78FNKK99 pKa = 10.86DD100 pKa = 3.04FDD102 pKa = 4.59LFCNEE107 pKa = 4.07AFDD110 pKa = 5.51RR111 pKa = 11.84SDD113 pKa = 5.36DD114 pKa = 3.95EE115 pKa = 5.69QEE117 pKa = 4.13PQPDD121 pKa = 3.53DD122 pKa = 3.73SAPSSSRR129 pKa = 11.84PPTPPGSQATPPKK142 pKa = 9.87KK143 pKa = 9.42KK144 pKa = 10.92AKK146 pKa = 9.08MDD148 pKa = 3.87SPNDD152 pKa = 3.51MPADD156 pKa = 3.4LMEE159 pKa = 4.54YY160 pKa = 9.82LSCAILSNKK169 pKa = 9.33TITCFLIYY177 pKa = 7.83TTLEE181 pKa = 3.97KK182 pKa = 10.95SEE184 pKa = 4.3LLYY187 pKa = 11.35NKK189 pKa = 10.09LSEE192 pKa = 4.24KK193 pKa = 10.15YY194 pKa = 9.15KK195 pKa = 9.79PRR197 pKa = 11.84FISRR201 pKa = 11.84HH202 pKa = 4.98KK203 pKa = 11.14YY204 pKa = 9.16EE205 pKa = 4.44GNSLIFIITGTKK217 pKa = 9.62HH218 pKa = 5.52RR219 pKa = 11.84VSAVFNYY226 pKa = 9.48CATYY230 pKa = 10.33CSVSFIVVKK239 pKa = 10.3GVIKK243 pKa = 9.98EE244 pKa = 4.04YY245 pKa = 10.34PLYY248 pKa = 10.78CHH250 pKa = 7.33LCVEE254 pKa = 5.15PYY256 pKa = 10.58SVLQEE261 pKa = 4.37SIEE264 pKa = 4.28GGLNSEE270 pKa = 4.78FFDD273 pKa = 4.78APEE276 pKa = 4.24DD277 pKa = 3.49AAKK280 pKa = 10.32NVNWVAISEE289 pKa = 4.34YY290 pKa = 10.72ALKK293 pKa = 10.55INCDD297 pKa = 4.12DD298 pKa = 3.99IYY300 pKa = 11.58LLMGLYY306 pKa = 10.09KK307 pKa = 10.25EE308 pKa = 4.59FQSPVPNCSKK318 pKa = 10.8CEE320 pKa = 3.79NRR322 pKa = 11.84MLTNHH327 pKa = 6.16FKK329 pKa = 10.46FHH331 pKa = 7.06KK332 pKa = 9.05EE333 pKa = 3.5HH334 pKa = 7.05HH335 pKa = 6.35EE336 pKa = 4.01NALLFAACRR345 pKa = 11.84NQKK348 pKa = 8.25TICQQAVDD356 pKa = 3.95GVIAQRR362 pKa = 11.84RR363 pKa = 11.84VDD365 pKa = 3.75MAQMTRR371 pKa = 11.84NEE373 pKa = 3.73QLAARR378 pKa = 11.84FDD380 pKa = 3.78KK381 pKa = 10.93LFEE384 pKa = 4.2RR385 pKa = 11.84LEE387 pKa = 4.62VILSAQSSYY396 pKa = 9.85TISMFMAGIVWFEE409 pKa = 3.82NLFPGQSFKK418 pKa = 11.48DD419 pKa = 3.53LLLEE423 pKa = 4.22LLEE426 pKa = 4.74CMVSNIPKK434 pKa = 9.07RR435 pKa = 11.84RR436 pKa = 11.84YY437 pKa = 8.45WLFTGPVNTGKK448 pKa = 7.5TTLAAAVLDD457 pKa = 4.08LCGGKK462 pKa = 9.79ALNINMPFDD471 pKa = 3.7KK472 pKa = 11.05LNFEE476 pKa = 4.93LGVAIDD482 pKa = 3.29QFMVVFEE489 pKa = 4.43DD490 pKa = 3.6VKK492 pKa = 11.02GQKK495 pKa = 9.5SEE497 pKa = 4.59NKK499 pKa = 9.81DD500 pKa = 3.53LPPGQGINNLDD511 pKa = 3.91NLRR514 pKa = 11.84DD515 pKa = 3.9HH516 pKa = 7.37LDD518 pKa = 3.48GAVKK522 pKa = 10.63VNLEE526 pKa = 4.2KK527 pKa = 10.85KK528 pKa = 9.73HH529 pKa = 6.21LNKK532 pKa = 9.17KK533 pKa = 6.38TQIFPPGIVTCNEE546 pKa = 3.51YY547 pKa = 10.7FIPFTLRR554 pKa = 11.84VRR556 pKa = 11.84FCKK559 pKa = 10.5KK560 pKa = 10.3LVFKK564 pKa = 10.48FSKK567 pKa = 10.14YY568 pKa = 9.87QYY570 pKa = 10.83LSLKK574 pKa = 9.16KK575 pKa = 8.41TEE577 pKa = 3.95CLGRR581 pKa = 11.84YY582 pKa = 9.44RR583 pKa = 11.84ILQNGCTLLLLLIYY597 pKa = 10.57HH598 pKa = 7.26CDD600 pKa = 3.48LDD602 pKa = 5.24DD603 pKa = 4.13FAEE606 pKa = 4.59SIQGKK611 pKa = 8.22VRR613 pKa = 11.84AWKK616 pKa = 10.01EE617 pKa = 3.82RR618 pKa = 11.84VNSEE622 pKa = 3.92ISVSTYY628 pKa = 11.01LEE630 pKa = 3.87MRR632 pKa = 11.84QCCLEE637 pKa = 4.11GKK639 pKa = 10.19DD640 pKa = 3.43ILSARR645 pKa = 11.84NTQMPTQHH653 pKa = 6.52NEE655 pKa = 3.35

Molecular weight: 75.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1386

160

655

346.5

39.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.916 ± 1.035

2.958 ± 0.977

5.628 ± 0.24

6.566 ± 0.502

4.69 ± 0.614

5.339 ± 0.893

1.804 ± 0.384

5.051 ± 0.547

6.349 ± 0.877

10.75 ± 0.959

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.886 ± 0.306

4.762 ± 0.513

5.916 ± 0.759

4.762 ± 0.533

4.834 ± 0.417

6.205 ± 0.387

4.762 ± 0.554

5.267 ± 0.998

1.443 ± 0.56

4.113 ± 0.611
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski