Helicobacter phage Pt4472G

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Schmidvirus; unclassified Schmidvirus

Average proteome isoelectric point is 7.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5RFK9|A0A1S5RFK9_9CAUD Uncharacterized protein OS=Helicobacter phage Pt4472G OX=1852675 PE=4 SV=1
MM1 pKa = 7.66GIKK4 pKa = 9.82EE5 pKa = 4.23KK6 pKa = 10.55EE7 pKa = 3.99IEE9 pKa = 4.18LEE11 pKa = 3.99TLKK14 pKa = 11.01RR15 pKa = 11.84EE16 pKa = 3.99IAQAEE21 pKa = 4.06ASLEE25 pKa = 3.89QDD27 pKa = 4.59FIKK30 pKa = 10.93HH31 pKa = 5.17MVDD34 pKa = 2.85KK35 pKa = 10.51TSEE38 pKa = 4.11KK39 pKa = 10.83VEE41 pKa = 4.46DD42 pKa = 3.98LFFSNKK48 pKa = 8.85PEE50 pKa = 4.27FYY52 pKa = 10.1RR53 pKa = 11.84FVFTEE58 pKa = 3.6QNNYY62 pKa = 9.57LRR64 pKa = 11.84EE65 pKa = 4.0KK66 pKa = 10.13LTDD69 pKa = 3.41KK70 pKa = 10.83VGRR73 pKa = 11.84AMDD76 pKa = 4.45LSDD79 pKa = 4.45EE80 pKa = 4.23IQRR83 pKa = 11.84DD84 pKa = 3.65RR85 pKa = 11.84EE86 pKa = 4.0NEE88 pKa = 3.96EE89 pKa = 3.62IEE91 pKa = 4.04KK92 pKa = 10.98DD93 pKa = 3.25KK94 pKa = 11.4EE95 pKa = 4.14AFLKK99 pKa = 10.17KK100 pKa = 10.08HH101 pKa = 6.12PEE103 pKa = 3.42IDD105 pKa = 3.8LNEE108 pKa = 4.37LLDD111 pKa = 4.19FYY113 pKa = 11.61NEE115 pKa = 4.19EE116 pKa = 3.62IPNRR120 pKa = 11.84FKK122 pKa = 11.17KK123 pKa = 10.58QIDD126 pKa = 3.72KK127 pKa = 11.52LEE129 pKa = 4.31GEE131 pKa = 4.73AFFEE135 pKa = 5.78AILDD139 pKa = 3.78YY140 pKa = 11.21FNALNSKK147 pKa = 10.19PEE149 pKa = 3.98EE150 pKa = 4.28GG151 pKa = 3.76

Molecular weight: 18.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5RFI4|A0A1S5RFI4_9CAUD Putative DNA helicase OS=Helicobacter phage Pt4472G OX=1852675 PE=4 SV=1
MM1 pKa = 7.44RR2 pKa = 11.84QRR4 pKa = 11.84NEE6 pKa = 3.62TATSFNKK13 pKa = 9.98LRR15 pKa = 11.84EE16 pKa = 3.99ITQAIQAQKK25 pKa = 11.18NSAQTSNNANTSEE38 pKa = 4.22ITSQSTPKK46 pKa = 10.32PKK48 pKa = 9.51GTARR52 pKa = 11.84TKK54 pKa = 11.0NSALKK59 pKa = 7.34PTKK62 pKa = 9.79RR63 pKa = 11.84AFSEE67 pKa = 4.09RR68 pKa = 11.84QRR70 pKa = 11.84ITIRR74 pKa = 11.84DD75 pKa = 3.6NSQASAIKK83 pKa = 9.95KK84 pKa = 9.91SKK86 pKa = 10.7GGAA89 pKa = 3.21

Molecular weight: 9.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

32

0

32

8707

69

1124

272.1

31.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.42 ± 0.387

0.792 ± 0.223

4.962 ± 0.301

8.717 ± 0.28

4.881 ± 0.353

4.341 ± 0.275

1.39 ± 0.2

6.925 ± 0.256

11.026 ± 0.477

10.543 ± 0.33

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.895 ± 0.176

7.936 ± 0.324

2.4 ± 0.192

4.422 ± 0.307

3.962 ± 0.194

6.271 ± 0.265

4.64 ± 0.239

4.433 ± 0.373

0.471 ± 0.085

3.572 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski