Bacillus phage PBC4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 123 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D6X8F6|A0A1D6X8F6_9CAUD Uncharacterized protein OS=Bacillus phage PBC4 OX=1675028 GN=PBC4_111 PE=4 SV=1
MM1 pKa = 7.44NPTIYY6 pKa = 10.9KK7 pKa = 9.9LGCEE11 pKa = 4.24FLEE14 pKa = 4.31TDD16 pKa = 3.77ANEE19 pKa = 3.98EE20 pKa = 4.05TLQEE24 pKa = 4.09IVNDD28 pKa = 3.99LTAEE32 pKa = 4.24LGDD35 pKa = 3.69AYY37 pKa = 9.92EE38 pKa = 4.61LEE40 pKa = 4.73EE41 pKa = 4.48ISEE44 pKa = 4.38KK45 pKa = 10.69LRR47 pKa = 11.84EE48 pKa = 3.86QGYY51 pKa = 7.74FANYY55 pKa = 9.79IEE57 pKa = 4.44INEE60 pKa = 4.22NIKK63 pKa = 10.97NYY65 pKa = 9.36ILEE68 pKa = 4.28YY69 pKa = 10.99NRR71 pKa = 11.84DD72 pKa = 3.34

Molecular weight: 8.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D6X885|A0A1D6X885_9CAUD Putative DNA primase OS=Bacillus phage PBC4 OX=1675028 GN=PBC4_042 PE=4 SV=1
MM1 pKa = 7.1ITYY4 pKa = 9.73TDD6 pKa = 3.41GTIFRR11 pKa = 11.84GRR13 pKa = 11.84LMVGQIWVKK22 pKa = 10.12GKK24 pKa = 10.18RR25 pKa = 11.84EE26 pKa = 3.84KK27 pKa = 11.03LIVGFDD33 pKa = 3.82EE34 pKa = 5.8FYY36 pKa = 10.31MYY38 pKa = 10.99YY39 pKa = 8.02QTKK42 pKa = 8.72TDD44 pKa = 3.65RR45 pKa = 11.84KK46 pKa = 8.86KK47 pKa = 10.3GTITGVRR54 pKa = 11.84RR55 pKa = 11.84SSFRR59 pKa = 11.84KK60 pKa = 8.47WLTSGAMLKK69 pKa = 9.82II70 pKa = 4.13

Molecular weight: 8.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

123

0

123

23941

49

2250

194.6

22.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.994 ± 0.428

0.911 ± 0.138

6.361 ± 0.205

8.011 ± 0.467

4.486 ± 0.2

6.533 ± 0.347

1.738 ± 0.166

6.975 ± 0.212

9.072 ± 0.36

7.581 ± 0.269

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.957 ± 0.197

6.257 ± 0.246

2.673 ± 0.198

3.63 ± 0.361

3.613 ± 0.158

5.359 ± 0.303

5.873 ± 0.338

6.516 ± 0.216

1.14 ± 0.093

4.319 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski