Klebsiella michiganensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Klebsiella

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5696 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J2KMH4|A0A0J2KMH4_9ENTR Thioredoxin OS=Klebsiella michiganensis OX=1134687 GN=AGG97_30660 PE=4 SV=1
MM1 pKa = 7.67NIGLFYY7 pKa = 10.91GSSTCYY13 pKa = 10.49TEE15 pKa = 3.84MAAEE19 pKa = 5.12KK20 pKa = 10.0IRR22 pKa = 11.84DD23 pKa = 3.65IIGPEE28 pKa = 4.02LVTLHH33 pKa = 6.12NLKK36 pKa = 10.49DD37 pKa = 3.85DD38 pKa = 4.22SPALMTQYY46 pKa = 11.26DD47 pKa = 4.24VLILGIPTWDD57 pKa = 3.26FGEE60 pKa = 4.22IQEE63 pKa = 4.43DD64 pKa = 4.16WEE66 pKa = 4.67AVWQQLDD73 pKa = 3.72TLDD76 pKa = 3.83LQGKK80 pKa = 8.79IVALYY85 pKa = 11.07GMGDD89 pKa = 3.29QLGYY93 pKa = 11.02GEE95 pKa = 4.87WFLDD99 pKa = 3.74ALGMLHH105 pKa = 7.54DD106 pKa = 4.49KK107 pKa = 10.83LATKK111 pKa = 9.99GVKK114 pKa = 10.29FIGYY118 pKa = 8.15WPTEE122 pKa = 4.1GYY124 pKa = 10.61EE125 pKa = 4.19FTSPKK130 pKa = 10.02PVIADD135 pKa = 3.41GQLFVGLALDD145 pKa = 3.64EE146 pKa = 4.38TNQYY150 pKa = 10.89DD151 pKa = 4.3LSDD154 pKa = 3.47EE155 pKa = 5.47RR156 pKa = 11.84IQNWCEE162 pKa = 3.76QILGEE167 pKa = 4.15MAEE170 pKa = 4.24QFSS173 pKa = 3.85

Molecular weight: 19.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A384I3E9|A0A384I3E9_9ENTR Head-tail adaptor protein OS=Klebsiella michiganensis OX=1134687 GN=SAMEA2273883_00822 PE=4 SV=1
MM1 pKa = 7.57ASVSISCPSCSATDD15 pKa = 3.7GVVRR19 pKa = 11.84NGKK22 pKa = 8.27STAGHH27 pKa = 4.88QRR29 pKa = 11.84YY30 pKa = 8.94LCSHH34 pKa = 6.46CRR36 pKa = 11.84KK37 pKa = 7.49TWQLQFTYY45 pKa = 9.73TASQPGTHH53 pKa = 6.22QKK55 pKa = 8.02ITDD58 pKa = 3.34MAMNGVGCRR67 pKa = 11.84ASARR71 pKa = 11.84IMGVGLNTVLRR82 pKa = 11.84HH83 pKa = 5.87LKK85 pKa = 10.37NSGRR89 pKa = 11.84SRR91 pKa = 3.37

Molecular weight: 9.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5696

0

5696

1795927

29

3993

315.3

34.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.181 ± 0.04

1.092 ± 0.014

5.11 ± 0.028

5.52 ± 0.029

3.888 ± 0.019

7.562 ± 0.03

2.232 ± 0.017

5.77 ± 0.026

4.003 ± 0.026

10.95 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.746 ± 0.015

3.579 ± 0.022

4.531 ± 0.024

4.4 ± 0.026

5.856 ± 0.028

6.002 ± 0.033

5.214 ± 0.022

7.006 ± 0.028

1.582 ± 0.014

2.776 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski