Lake Baikal phage Baikal-20-5m-C28

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 234 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4N7V7|A0A2H4N7V7_9CAUD Uncharacterized protein OS=Lake Baikal phage Baikal-20-5m-C28 OX=2047876 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.38LVISTQIHH10 pKa = 5.83EE11 pKa = 4.67NYY13 pKa = 9.41GAHH16 pKa = 6.94DD17 pKa = 3.58WDD19 pKa = 4.28GTGEE23 pKa = 4.11CPQYY27 pKa = 9.9WKK29 pKa = 11.05AKK31 pKa = 9.38GGHH34 pKa = 6.08EE35 pKa = 3.9YY36 pKa = 9.95MIEE39 pKa = 4.08GVPLNGVDD47 pKa = 3.43YY48 pKa = 11.26ASIVEE53 pKa = 4.06MANIGEE59 pKa = 4.18DD60 pKa = 3.12NEE62 pKa = 4.58YY63 pKa = 10.9YY64 pKa = 10.28RR65 pKa = 11.84EE66 pKa = 4.1TVLGWSIEE74 pKa = 4.08SDD76 pKa = 4.46DD77 pKa = 3.76YY78 pKa = 11.49MSWFEE83 pKa = 4.39KK84 pKa = 10.77SQLEE88 pKa = 3.87YY89 pKa = 11.33DD90 pKa = 3.83GVIKK94 pKa = 10.82YY95 pKa = 10.01KK96 pKa = 10.33EE97 pKa = 3.69PRR99 pKa = 11.84IDD101 pKa = 3.76YY102 pKa = 10.68NEE104 pKa = 3.87LNARR108 pKa = 11.84YY109 pKa = 8.17EE110 pKa = 4.08DD111 pKa = 3.59PMAYY115 pKa = 10.2AEE117 pKa = 4.14NAADD121 pKa = 4.13NDD123 pKa = 4.54AIHH126 pKa = 6.45YY127 pKa = 7.58GAA129 pKa = 5.2

Molecular weight: 14.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4N7S5|A0A2H4N7S5_9CAUD Uncharacterized protein OS=Lake Baikal phage Baikal-20-5m-C28 OX=2047876 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 9.19NTRR5 pKa = 11.84TTNYY9 pKa = 9.68IFTADD14 pKa = 4.03PLSVTDD20 pKa = 3.85MQQITIVKK28 pKa = 8.38KK29 pKa = 7.83TVKK32 pKa = 10.48AMNDD36 pKa = 3.19TAMQSYY42 pKa = 9.79KK43 pKa = 9.6YY44 pKa = 10.28AYY46 pKa = 8.58MRR48 pKa = 11.84ASVTGQQFPKK58 pKa = 10.27KK59 pKa = 8.6PTRR62 pKa = 11.84QRR64 pKa = 11.84VRR66 pKa = 11.84LMGRR70 pKa = 11.84GPRR73 pKa = 11.84RR74 pKa = 11.84IAEE77 pKa = 4.57RR78 pKa = 11.84IDD80 pKa = 3.06GLRR83 pKa = 11.84RR84 pKa = 11.84GSYY87 pKa = 10.05DD88 pKa = 2.74AYY90 pKa = 10.59LPQRR94 pKa = 11.84HH95 pKa = 5.72AVTFDD100 pKa = 3.07VYY102 pKa = 10.78IADD105 pKa = 3.62VRR107 pKa = 3.86

Molecular weight: 12.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

234

0

234

53038

29

2160

226.7

25.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.795 ± 0.198

1.086 ± 0.098

6.246 ± 0.121

6.075 ± 0.179

4.233 ± 0.117

6.624 ± 0.242

2.07 ± 0.149

7.444 ± 0.162

7.084 ± 0.269

7.672 ± 0.15

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.576 ± 0.101

5.922 ± 0.151

3.601 ± 0.095

3.494 ± 0.098

3.697 ± 0.132

6.756 ± 0.177

7.042 ± 0.305

6.128 ± 0.138

1.276 ± 0.071

4.18 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski