Polyporus brumalis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Polyporales; Polyporaceae; Polyporus

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18174 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A371CGF6|A0A371CGF6_9APHY Uncharacterized protein (Fragment) OS=Polyporus brumalis OX=139420 GN=OH76DRAFT_1491264 PE=4 SV=1
MM1 pKa = 7.32EE2 pKa = 5.16AADD5 pKa = 3.76SGFVPMYY12 pKa = 10.38LDD14 pKa = 4.91SSPLDD19 pKa = 3.88DD20 pKa = 4.64AQGSGYY26 pKa = 10.56AEE28 pKa = 4.52CWDD31 pKa = 3.94SPRR34 pKa = 11.84ARR36 pKa = 11.84HH37 pKa = 6.1SVLANPYY44 pKa = 8.58KK45 pKa = 9.78PARR48 pKa = 11.84ISFYY52 pKa = 10.37PLSDD56 pKa = 3.34NMGSSLPPRR65 pKa = 11.84FASPGMPPLPSSHH78 pKa = 6.59PQLYY82 pKa = 9.05QQVDD86 pKa = 3.68TTFFCCSPEE95 pKa = 3.93PMSPQSQVAASFQLSTPSYY114 pKa = 10.97LEE116 pKa = 4.36LPPPLAGAEE125 pKa = 4.43FISDD129 pKa = 4.46LPTAQPALEE138 pKa = 4.36SMEE141 pKa = 4.85SPLPPSDD148 pKa = 3.79LSEE151 pKa = 4.5FSWHH155 pKa = 5.4TSSRR159 pKa = 11.84LLEE162 pKa = 4.52SDD164 pKa = 4.95DD165 pKa = 3.8VPQASFTMSPGEE177 pKa = 4.02SWIEE181 pKa = 3.63EE182 pKa = 4.24QPFGEE187 pKa = 4.91HH188 pKa = 7.81DD189 pKa = 4.83DD190 pKa = 3.98DD191 pKa = 4.39TSDD194 pKa = 3.15GSAYY198 pKa = 10.38SLPGGRR204 pKa = 11.84THH206 pKa = 8.19PILFDD211 pKa = 3.33EE212 pKa = 5.09AEE214 pKa = 4.33WNGATWDD221 pKa = 4.08AVCLADD227 pKa = 4.59FLGGLFEE234 pKa = 6.02KK235 pKa = 10.63ADD237 pKa = 3.64PWSTLDD243 pKa = 4.98DD244 pKa = 4.37FLDD247 pKa = 4.23VPSLLPDD254 pKa = 4.31LPPTSTSLFLLASDD268 pKa = 4.18RR269 pKa = 11.84GGVGYY274 pKa = 10.52VATPRR279 pKa = 11.84SPDD282 pKa = 3.18WQDD285 pKa = 3.76LSSQTLAEE293 pKa = 4.3EE294 pKa = 4.43TRR296 pKa = 11.84ACTPLFSVSCGTEE309 pKa = 3.58ALEE312 pKa = 5.12DD313 pKa = 3.93VPAVDD318 pKa = 4.96SDD320 pKa = 4.21PPSSGDD326 pKa = 3.14TAYY329 pKa = 10.99DD330 pKa = 3.56DD331 pKa = 4.2TPEE334 pKa = 3.97EE335 pKa = 4.05AQAYY339 pKa = 8.93LPRR342 pKa = 11.84AEE344 pKa = 4.62YY345 pKa = 10.59EE346 pKa = 4.19PLSAPAMPSPSPVEE360 pKa = 4.03MLVKK364 pKa = 10.37PPGVTVRR371 pKa = 11.84GPCASIPAAEE381 pKa = 4.52EE382 pKa = 4.17DD383 pKa = 3.83SDD385 pKa = 4.67VLEE388 pKa = 4.81EE389 pKa = 4.32KK390 pKa = 10.4CGHH393 pKa = 6.59DD394 pKa = 4.32ASLGTDD400 pKa = 3.42GADD403 pKa = 2.92AAVEE407 pKa = 4.07RR408 pKa = 11.84GIEE411 pKa = 4.06TGVPEE416 pKa = 4.47SFDD419 pKa = 3.7RR420 pKa = 11.84DD421 pKa = 3.65VSVDD425 pKa = 3.69GPSLFADD432 pKa = 4.41DD433 pKa = 6.37DD434 pKa = 4.27VDD436 pKa = 6.16LDD438 pKa = 4.75DD439 pKa = 4.61EE440 pKa = 4.53

Molecular weight: 47.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A371DFU0|A0A371DFU0_9APHY Laccase C OS=Polyporus brumalis OX=139420 GN=OH76DRAFT_1417405 PE=3 SV=1
RR1 pKa = 7.38LHH3 pKa = 6.34KK4 pKa = 10.08QVPGLARR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 5.32KK14 pKa = 10.06QVPGLARR21 pKa = 11.84RR22 pKa = 11.84HH23 pKa = 5.32KK24 pKa = 10.06QVPGLARR31 pKa = 11.84RR32 pKa = 11.84HH33 pKa = 5.32KK34 pKa = 10.06QVPGLARR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 5.32KK44 pKa = 10.06QVPGLARR51 pKa = 11.84RR52 pKa = 11.84HH53 pKa = 4.96KK54 pKa = 10.19QQ55 pKa = 2.94

Molecular weight: 6.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18174

0

18174

7177947

49

5041

395.0

43.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.407 ± 0.018

1.459 ± 0.008

5.626 ± 0.013

5.732 ± 0.02

3.53 ± 0.01

6.517 ± 0.016

2.713 ± 0.009

4.252 ± 0.013

3.922 ± 0.017

9.242 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.06 ± 0.007

2.881 ± 0.009

6.946 ± 0.022

3.564 ± 0.013

7.028 ± 0.017

8.396 ± 0.023

5.973 ± 0.013

6.599 ± 0.015

1.512 ± 0.007

2.641 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski