Bacillus phage vB_BpsM-61

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Saundersvirus; unclassified Saundersvirus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3BXB3|A0A3G3BXB3_9CAUD Uncharacterized protein OS=Bacillus phage vB_BpsM-61 OX=2419620 GN=BpsM61_00002 PE=4 SV=1
MM1 pKa = 8.4DD2 pKa = 4.57YY3 pKa = 10.94GDD5 pKa = 4.7KK6 pKa = 11.04VGEE9 pKa = 4.36CEE11 pKa = 4.04CCDD14 pKa = 4.05RR15 pKa = 11.84EE16 pKa = 4.48GTPIQYY22 pKa = 10.2SRR24 pKa = 11.84YY25 pKa = 9.11FIGWLCEE32 pKa = 4.07NCWEE36 pKa = 4.66SIAEE40 pKa = 4.16SVISGEE46 pKa = 4.04

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3BY10|A0A3G3BY10_9CAUD Uncharacterized protein OS=Bacillus phage vB_BpsM-61 OX=2419620 GN=BpsM61_00047 PE=4 SV=1
MM1 pKa = 7.67GKK3 pKa = 9.47PSKK6 pKa = 9.8GTPKK10 pKa = 10.87DD11 pKa = 3.35MRR13 pKa = 11.84LKK15 pKa = 10.34EE16 pKa = 4.15NKK18 pKa = 9.57KK19 pKa = 10.33LRR21 pKa = 11.84GPSKK25 pKa = 10.08RR26 pKa = 11.84SPGAASTKK34 pKa = 9.9KK35 pKa = 10.44KK36 pKa = 10.33KK37 pKa = 10.41

Molecular weight: 4.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

14855

32

1040

198.1

22.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.412 ± 0.384

0.545 ± 0.104

6.94 ± 0.261

7.506 ± 0.431

3.601 ± 0.169

7.412 ± 0.24

2.067 ± 0.191

7.156 ± 0.293

7.486 ± 0.466

6.738 ± 0.214

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.669 ± 0.172

5.136 ± 0.213

3.568 ± 0.203

4.012 ± 0.158

5.5 ± 0.211

6.038 ± 0.212

5.816 ± 0.241

6.254 ± 0.222

1.508 ± 0.114

3.635 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski