Sphingomonas sp. WG

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3630 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0W1QQH9|A0A0W1QQH9_9SPHN Uncharacterized protein OS=Sphingomonas sp. WG OX=1592629 GN=ATB93_00285 PE=4 SV=1
MM1 pKa = 7.9GDD3 pKa = 3.14QILNYY8 pKa = 8.74RR9 pKa = 11.84ACGAEE14 pKa = 4.34CRR16 pKa = 11.84EE17 pKa = 3.95DD18 pKa = 3.64WLRR21 pKa = 11.84MLACDD26 pKa = 4.68DD27 pKa = 4.06GLPFEE32 pKa = 4.71SVRR35 pKa = 11.84DD36 pKa = 3.37AAEE39 pKa = 3.94RR40 pKa = 11.84FGEE43 pKa = 4.5AEE45 pKa = 4.72DD46 pKa = 4.22FEE48 pKa = 4.64QLIDD52 pKa = 3.63YY53 pKa = 10.85CEE55 pKa = 4.73GGGSALQQ62 pKa = 3.5

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0W1QGJ8|A0A0W1QGJ8_9SPHN Uncharacterized protein OS=Sphingomonas sp. WG OX=1592629 GN=ATB93_02670 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3630

0

3630

1189672

29

2191

327.7

35.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.906 ± 0.062

0.715 ± 0.013

5.744 ± 0.027

5.194 ± 0.041

3.538 ± 0.023

8.99 ± 0.039

1.943 ± 0.02

4.736 ± 0.023

2.72 ± 0.028

10.109 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.27 ± 0.019

2.509 ± 0.028

5.45 ± 0.029

3.248 ± 0.023

7.583 ± 0.043

5.009 ± 0.029

5.384 ± 0.034

7.25 ± 0.031

1.478 ± 0.019

2.225 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski