Arthrobacter phage Eileen

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bridgettevirus; Arthrobacter virus Eileen

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2KFQ1|A0A3G2KFQ1_9CAUD Helix-turn-helix DNA binding protein OS=Arthrobacter phage Eileen OX=2419956 GN=35 PE=4 SV=1
MM1 pKa = 7.71AIEE4 pKa = 4.37TTQDD8 pKa = 3.2GYY10 pKa = 11.4QKK12 pKa = 10.57PDD14 pKa = 2.98GNEE17 pKa = 3.7LVRR20 pKa = 11.84GGDD23 pKa = 3.44NVIAANAQKK32 pKa = 10.79AQDD35 pKa = 3.74HH36 pKa = 4.8YY37 pKa = 10.3TEE39 pKa = 4.36VKK41 pKa = 10.63ADD43 pKa = 3.54ALTLAGRR50 pKa = 11.84LGVAEE55 pKa = 4.13AMINAGAGGPGVSEE69 pKa = 5.37DD70 pKa = 3.78PDD72 pKa = 4.13HH73 pKa = 7.23PGLYY77 pKa = 9.98YY78 pKa = 10.58FAGPAFAADD87 pKa = 3.88PLHH90 pKa = 6.6PGLYY94 pKa = 9.96LFEE97 pKa = 5.08EE98 pKa = 4.69STPP101 pKa = 3.58

Molecular weight: 10.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2KFW1|A0A3G2KFW1_9CAUD Uncharacterized protein OS=Arthrobacter phage Eileen OX=2419956 GN=60 PE=4 SV=1
MM1 pKa = 7.66PSAPRR6 pKa = 11.84KK7 pKa = 7.95PCPTAGCPEE16 pKa = 4.88LLPPGQRR23 pKa = 11.84RR24 pKa = 11.84CPTHH28 pKa = 6.13ATQHH32 pKa = 4.75EE33 pKa = 4.32QARR36 pKa = 11.84GSRR39 pKa = 11.84TQRR42 pKa = 11.84GYY44 pKa = 11.3DD45 pKa = 3.29ATHH48 pKa = 7.08DD49 pKa = 3.88ALRR52 pKa = 11.84KK53 pKa = 8.62QWAPHH58 pKa = 5.0VATGSVRR65 pKa = 11.84CARR68 pKa = 11.84CGKK71 pKa = 9.28PILAGTPWDD80 pKa = 4.6LGHH83 pKa = 6.87TDD85 pKa = 6.05DD86 pKa = 4.01RR87 pKa = 11.84TTWTGPEE94 pKa = 3.44HH95 pKa = 6.51AACNRR100 pKa = 11.84SAGGKK105 pKa = 6.78ATKK108 pKa = 10.11HH109 pKa = 5.55RR110 pKa = 11.84GHH112 pKa = 6.76RR113 pKa = 11.84RR114 pKa = 3.18

Molecular weight: 12.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

13105

30

1298

208.0

22.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.133 ± 0.572

0.969 ± 0.164

6.105 ± 0.182

5.486 ± 0.358

2.442 ± 0.135

8.493 ± 0.469

2.037 ± 0.246

4.273 ± 0.181

4.556 ± 0.304

7.86 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.045 ± 0.128

3.075 ± 0.184

5.822 ± 0.467

4.098 ± 0.241

6.57 ± 0.382

5.41 ± 0.304

7.188 ± 0.44

6.852 ± 0.374

2.007 ± 0.209

2.579 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski