Mycobacterium phage Philly

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; Pipefishvirus; unclassified Pipefishvirus

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TSI2|A0A5J6TSI2_9CAUD Uncharacterized protein OS=Mycobacterium phage Philly OX=2599874 GN=16 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.15CVIGIPLRR10 pKa = 11.84PEE12 pKa = 3.68MLMAAGPYY20 pKa = 9.48LRR22 pKa = 11.84NAAEE26 pKa = 5.24DD27 pKa = 3.75AVQMQAEE34 pKa = 4.33TLGEE38 pKa = 4.09IVLGDD43 pKa = 3.46VDD45 pKa = 5.0LAYY48 pKa = 10.54CGPVNAIPDD57 pKa = 3.75EE58 pKa = 4.35YY59 pKa = 11.67AWLTNGAPQFFVIAVYY75 pKa = 10.27VAEE78 pKa = 4.65ISDD81 pKa = 3.46ARR83 pKa = 3.8

Molecular weight: 8.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TSV1|A0A5J6TSV1_9CAUD Uncharacterized protein OS=Mycobacterium phage Philly OX=2599874 GN=87 PE=4 SV=1
MM1 pKa = 7.51ARR3 pKa = 11.84QPGRR7 pKa = 11.84SKK9 pKa = 10.75SLKK12 pKa = 10.52RR13 pKa = 11.84PDD15 pKa = 3.22IYY17 pKa = 11.13DD18 pKa = 3.5ALRR21 pKa = 11.84RR22 pKa = 11.84KK23 pKa = 10.12GMSKK27 pKa = 9.17TKK29 pKa = 10.2AARR32 pKa = 11.84ISNAVANRR40 pKa = 11.84TIRR43 pKa = 11.84HH44 pKa = 5.87RR45 pKa = 11.84GGKK48 pKa = 9.45KK49 pKa = 9.15GRR51 pKa = 11.84KK52 pKa = 7.85HH53 pKa = 5.03KK54 pKa = 10.42RR55 pKa = 11.84GRR57 pKa = 11.84GKK59 pKa = 10.26RR60 pKa = 11.84RR61 pKa = 11.84AA62 pKa = 3.37

Molecular weight: 7.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

102

0

102

21850

32

1909

214.2

23.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.414 ± 0.32

1.162 ± 0.168

6.915 ± 0.256

5.986 ± 0.252

2.604 ± 0.109

9.465 ± 0.653

1.73 ± 0.142

4.783 ± 0.179

3.217 ± 0.173

7.881 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.211 ± 0.127

2.87 ± 0.134

6.087 ± 0.226

3.112 ± 0.145

6.686 ± 0.369

5.346 ± 0.2

6.503 ± 0.18

7.757 ± 0.25

1.991 ± 0.146

2.279 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski