Chlamydia phage 4

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Gokushovirinae; Chlamydiamicrovirus; unclassified Chlamydiamicrovirus

Average proteome isoelectric point is 7.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q3ZJZ5|Q3ZJZ5_9VIRU Putative replication protein OS=Chlamydia phage 4 OX=313629 GN=ORF4 PE=4 SV=1
MM1 pKa = 7.56KK2 pKa = 10.45VFTVFDD8 pKa = 3.96IKK10 pKa = 10.2TEE12 pKa = 4.12IYY14 pKa = 9.28QQPFFMQATGAAIRR28 pKa = 11.84AFSDD32 pKa = 3.44MVNEE36 pKa = 5.09DD37 pKa = 3.17PTKK40 pKa = 10.75NQFAAHH46 pKa = 6.67PEE48 pKa = 3.85DD49 pKa = 4.43YY50 pKa = 10.27ILYY53 pKa = 9.96EE54 pKa = 4.11IGSYY58 pKa = 10.93DD59 pKa = 3.83DD60 pKa = 3.38STGTFIPLDD69 pKa = 3.6VPKK72 pKa = 10.9ALGTGLDD79 pKa = 4.24FKK81 pKa = 11.12HH82 pKa = 6.2KK83 pKa = 9.87QQ84 pKa = 3.07

Molecular weight: 9.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q3ZJZ7|Q3ZJZ7_9VIRU Putative capsid protein OS=Chlamydia phage 4 OX=313629 GN=VP3 PE=4 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84RR4 pKa = 11.84YY5 pKa = 9.23RR6 pKa = 11.84LSRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84SRR13 pKa = 11.84RR14 pKa = 11.84LFSRR18 pKa = 11.84TALRR22 pKa = 11.84MHH24 pKa = 6.81RR25 pKa = 11.84RR26 pKa = 11.84NRR28 pKa = 11.84LRR30 pKa = 11.84RR31 pKa = 11.84IMRR34 pKa = 11.84GGIRR38 pKa = 11.84FF39 pKa = 3.47

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1410

32

554

176.3

20.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.809 ± 1.521

1.702 ± 0.693

5.319 ± 0.892

5.745 ± 0.893

5.035 ± 0.589

5.957 ± 1.099

1.702 ± 0.45

4.255 ± 0.627

5.745 ± 1.138

8.156 ± 0.723

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.837 ± 0.721

4.326 ± 0.695

5.603 ± 0.852

5.248 ± 0.482

7.66 ± 1.715

7.801 ± 0.842

4.965 ± 0.933

5.035 ± 0.673

1.56 ± 0.424

4.539 ± 0.721
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski