Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Pectobacteriaceae; Pectobacterium; Pectobacterium atrosepticum

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4459 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6D5M2|Q6D5M2_PECAS Uncharacterized protein OS=Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) OX=218491 GN=ECA2019 PE=4 SV=1
MM1 pKa = 7.73LEE3 pKa = 4.45NPDD6 pKa = 3.65AFNFSFKK13 pKa = 10.93LLDD16 pKa = 3.57IEE18 pKa = 5.1DD19 pKa = 4.3RR20 pKa = 11.84PSVACMCSFCPRR32 pKa = 11.84TEE34 pKa = 3.8WEE36 pKa = 3.64EE37 pKa = 4.33DD38 pKa = 3.44EE39 pKa = 4.56EE40 pKa = 4.78HH41 pKa = 6.34FAEE44 pKa = 5.93GPTLNIEE51 pKa = 4.21MLQNFHH57 pKa = 6.9LRR59 pKa = 11.84DD60 pKa = 3.66SEE62 pKa = 4.99LDD64 pKa = 3.69GNTLKK69 pKa = 10.96YY70 pKa = 10.81ALYY73 pKa = 10.33AVLFFIIEE81 pKa = 4.34TQCTGVRR88 pKa = 11.84LIEE91 pKa = 4.85PINDD95 pKa = 3.27SVANYY100 pKa = 10.03YY101 pKa = 10.48LEE103 pKa = 4.5QGFEE107 pKa = 4.86DD108 pKa = 3.19ITGGSKK114 pKa = 10.7AILWRR119 pKa = 11.84SSEE122 pKa = 5.37NLLQWLGEE130 pKa = 4.23YY131 pKa = 9.98IQVVNDD137 pKa = 3.93DD138 pKa = 4.01VEE140 pKa = 5.42DD141 pKa = 4.27EE142 pKa = 4.53YY143 pKa = 11.73FLGDD147 pKa = 3.56NEE149 pKa = 4.84LNQNEE154 pKa = 4.46FNGGEE159 pKa = 4.05NGNN162 pKa = 3.84

Molecular weight: 18.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6CZ19|Q6CZ19_PECAS Methyl-accepting chemotaxis protein OS=Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) OX=218491 GN=ECA4334 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.41GRR39 pKa = 11.84TRR41 pKa = 11.84LSVSKK46 pKa = 10.96

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4459

0

4459

1442513

31

7523

323.5

35.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.513 ± 0.039

1.022 ± 0.014

5.306 ± 0.032

5.535 ± 0.03

3.783 ± 0.029

7.206 ± 0.034

2.292 ± 0.02

5.959 ± 0.029

4.133 ± 0.031

10.955 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.607 ± 0.019

3.818 ± 0.034

4.412 ± 0.027

4.715 ± 0.029

5.631 ± 0.034

6.363 ± 0.033

5.537 ± 0.045

6.969 ± 0.04

1.386 ± 0.016

2.858 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski