Leifsonia sp. Leaf336

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leifsonia; unclassified Leifsonia

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3857 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5MDL0|A0A0Q5MDL0_9MICO Hydroxyacid dehydrogenase OS=Leifsonia sp. Leaf336 OX=1736341 GN=ASF88_08080 PE=4 SV=1
MM1 pKa = 7.49LMSITAGAAKK11 pKa = 10.27RR12 pKa = 11.84STRR15 pKa = 11.84STLAFAAIIALLMTMLAVTSTNSSPADD42 pKa = 3.58AAQPWCGGTTVFGVVPDD59 pKa = 5.15DD60 pKa = 4.21PAASPTNTALVEE72 pKa = 4.07IDD74 pKa = 3.4GSSVGNAQLSGSLVTRR90 pKa = 11.84SPGDD94 pKa = 3.37TNLQNGLGLTADD106 pKa = 3.94GSTAYY111 pKa = 10.29AGADD115 pKa = 2.92NGTTIRR121 pKa = 11.84RR122 pKa = 11.84YY123 pKa = 11.27DD124 pKa = 3.73MLTDD128 pKa = 3.46TWTSYY133 pKa = 10.69PGRR136 pKa = 11.84PLPAGGEE143 pKa = 4.41GVVAGAVDD151 pKa = 4.31PVSGIYY157 pKa = 10.39YY158 pKa = 9.9FGEE161 pKa = 4.43IGAGPTDD168 pKa = 3.7PTLGHH173 pKa = 7.05LWGFDD178 pKa = 3.23TNTNQLISPAPLITFPSVAGEE199 pKa = 4.12NNGDD203 pKa = 3.49IAFDD207 pKa = 3.81GAGNLYY213 pKa = 9.98IVTSSTTHH221 pKa = 6.34GDD223 pKa = 3.69LEE225 pKa = 4.65VSPGPLPTTAAQAQLLQPTLLSGFTVPPAAGAAVNGIAFDD265 pKa = 3.8ATGTLYY271 pKa = 11.07LSLSGATGGVFSIDD285 pKa = 3.78PGTGAPQSALTPLSAALIGPSGTGLVDD312 pKa = 4.28LASCSFPPSVSAQKK326 pKa = 10.75NVLSRR331 pKa = 11.84VNPTDD336 pKa = 3.15QFTLSVTGPTVSLPATATTVGTADD360 pKa = 4.66GLQQTAGGQIVQAGPALARR379 pKa = 11.84FSDD382 pKa = 4.41TYY384 pKa = 10.66TATEE388 pKa = 4.25TASGTTDD395 pKa = 3.6LSQYY399 pKa = 8.65STTYY403 pKa = 10.16SCVDD407 pKa = 3.19VVNPTNPQFPITGSGTTVSFPLDD430 pKa = 3.61PFVGEE435 pKa = 4.47SPSVVCTFTNAALTSALTLEE455 pKa = 4.72KK456 pKa = 9.53TASPTAVSTVGQTVTYY472 pKa = 10.23SFLVTNTGSTILTSIAVAEE491 pKa = 4.38TAFSGTGTLPAPTCPTTTLDD511 pKa = 4.25PGDD514 pKa = 3.96STICTAAYY522 pKa = 9.96DD523 pKa = 3.69VTAADD528 pKa = 3.42IAAGVIDD535 pKa = 4.24NTAEE539 pKa = 4.1ANGLDD544 pKa = 3.96PSNDD548 pKa = 3.29TVTSPEE554 pKa = 4.19DD555 pKa = 3.51SAQVTATPAVAALDD569 pKa = 3.81LTKK572 pKa = 9.88TANPATVTAAGDD584 pKa = 3.74TITYY588 pKa = 7.38TFHH591 pKa = 5.54VTNTGNVPIDD601 pKa = 3.76SIAIQEE607 pKa = 4.52GNFSGTGSISAITCNATTLAPAASTDD633 pKa = 3.52CTATYY638 pKa = 9.16XITEE642 pKa = 4.26VVVLWHH648 pKa = 7.16RR649 pKa = 11.84DD650 pKa = 3.09RR651 pKa = 11.84GRR653 pKa = 3.89

Molecular weight: 65.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5M3B3|A0A0Q5M3B3_9MICO Phosphoglucomutase OS=Leifsonia sp. Leaf336 OX=1736341 GN=ASF88_16840 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84KK12 pKa = 8.98RR13 pKa = 11.84AKK15 pKa = 8.46THH17 pKa = 5.11GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.52GRR40 pKa = 11.84EE41 pKa = 3.9KK42 pKa = 10.95LSAA45 pKa = 3.78

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3857

0

3857

1226354

27

2233

318.0

33.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.502 ± 0.056

0.472 ± 0.01

6.041 ± 0.033

5.421 ± 0.047

3.26 ± 0.023

9.029 ± 0.037

1.933 ± 0.016

4.445 ± 0.03

2.035 ± 0.028

10.261 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.648 ± 0.014

2.088 ± 0.025

5.41 ± 0.027

2.816 ± 0.023

7.072 ± 0.048

5.793 ± 0.029

6.167 ± 0.041

8.987 ± 0.038

1.524 ± 0.019

2.095 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski