Streptococcus phage phiJH1301-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9I6I5|A0A1X9I6I5_9CAUD Uncharacterized protein OS=Streptococcus phage phiJH1301-1 OX=1860179 PE=4 SV=1
MM1 pKa = 7.68EE2 pKa = 4.49GTYY5 pKa = 10.23PWFDD9 pKa = 3.49YY10 pKa = 11.19DD11 pKa = 5.36RR12 pKa = 11.84DD13 pKa = 3.88YY14 pKa = 11.48LQPDD18 pKa = 4.07EE19 pKa = 4.78PRR21 pKa = 11.84QVHH24 pKa = 7.25DD25 pKa = 3.72PDD27 pKa = 2.99EE28 pKa = 4.13WVFRR32 pKa = 11.84GGQWIYY38 pKa = 11.49VGDD41 pKa = 3.78AA42 pKa = 3.3

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9I676|A0A1X9I676_9CAUD Uncharacterized protein OS=Streptococcus phage phiJH1301-1 OX=1860179 PE=4 SV=1
MM1 pKa = 7.14KK2 pKa = 10.36QLLRR6 pKa = 11.84SFGFILIIYY15 pKa = 7.68LFVPNAIRR23 pKa = 11.84EE24 pKa = 4.04MTLAQKK30 pKa = 10.62IMFGLGASWLFYY42 pKa = 10.71EE43 pKa = 5.55GGRR46 pKa = 11.84KK47 pKa = 8.72

Molecular weight: 5.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

13386

41

1353

185.9

20.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.388 ± 0.283

0.583 ± 0.106

6.26 ± 0.228

7.695 ± 0.517

3.922 ± 0.327

6.679 ± 0.483

1.367 ± 0.131

6.679 ± 0.212

7.822 ± 0.366

8.098 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.211 ± 0.163

5.095 ± 0.218

3.048 ± 0.18

4.049 ± 0.158

4.863 ± 0.309

5.902 ± 0.341

6.223 ± 0.279

6.821 ± 0.224

1.412 ± 0.15

3.885 ± 0.298
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski