Pseudomonas phage VW-6B

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1GNV4|A0A2D1GNV4_9CAUD Tail protein OS=Pseudomonas phage VW-6B OX=2041486 GN=VW6B_41 PE=4 SV=1
MM1 pKa = 6.23VTDD4 pKa = 4.18YY5 pKa = 11.06LFCEE9 pKa = 4.17PLLIGRR15 pKa = 11.84LRR17 pKa = 11.84DD18 pKa = 3.52AVPEE22 pKa = 4.06FVEE25 pKa = 4.47VTGVAGLSQMNEE37 pKa = 4.16DD38 pKa = 4.25NPVCPMAYY46 pKa = 10.37VMYY49 pKa = 10.77LGDD52 pKa = 3.88TVNTSTAATGGSQRR66 pKa = 11.84RR67 pKa = 11.84LQFVTQLWAVVICVYY82 pKa = 10.44FADD85 pKa = 4.37GRR87 pKa = 11.84GLGADD92 pKa = 3.47ISSEE96 pKa = 4.09AGPLMLKK103 pKa = 9.01TVEE106 pKa = 5.2ALAGWSPLEE115 pKa = 4.37GVTRR119 pKa = 11.84PLGRR123 pKa = 11.84SNQSLPAQYY132 pKa = 10.55EE133 pKa = 4.17SGYY136 pKa = 10.9GYY138 pKa = 10.87YY139 pKa = 10.05PLVFQVDD146 pKa = 3.73VPAAIGGYY154 pKa = 8.9RR155 pKa = 3.22

Molecular weight: 16.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1GNJ2|A0A2D1GNJ2_9CAUD Head morphogenesis protein OS=Pseudomonas phage VW-6B OX=2041486 GN=VW6B_28 PE=4 SV=1
MM1 pKa = 7.37GRR3 pKa = 11.84RR4 pKa = 11.84ASRR7 pKa = 11.84YY8 pKa = 8.18RR9 pKa = 11.84RR10 pKa = 11.84GPATSAEE17 pKa = 4.04MALLSEE23 pKa = 4.27INEE26 pKa = 3.87RR27 pKa = 11.84LARR30 pKa = 11.84MEE32 pKa = 4.52GNLDD36 pKa = 3.75DD37 pKa = 4.7VKK39 pKa = 11.25SSAIRR44 pKa = 11.84QGAIAGAISGGVSGGLVYY62 pKa = 7.84TTILLIKK69 pKa = 10.15AKK71 pKa = 10.53LGVGG75 pKa = 3.55

Molecular weight: 7.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

11369

38

1238

247.2

26.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.171 ± 0.793

0.756 ± 0.124

5.867 ± 0.239

5.673 ± 0.222

2.955 ± 0.225

8.075 ± 0.323

1.654 ± 0.169

4.864 ± 0.262

4.706 ± 0.363

9.737 ± 0.304

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.181 ± 0.158

3.738 ± 0.166

4.442 ± 0.363

4.609 ± 0.291

5.884 ± 0.307

6.166 ± 0.277

6.658 ± 0.383

6.527 ± 0.286

1.583 ± 0.18

2.753 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski