Paenibacillus sp. VKM B-2647

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5046 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C2QMG3|A0A0C2QMG3_9BACL DNA repair protein RadC OS=Paenibacillus sp. VKM B-2647 OX=1590651 GN=SD70_21050 PE=3 SV=1
MM1 pKa = 7.8SDD3 pKa = 3.09QPIFFEE9 pKa = 4.72FEE11 pKa = 3.9QEE13 pKa = 3.72RR14 pKa = 11.84SAFLAMDD21 pKa = 4.31TLQEE25 pKa = 4.06LGYY28 pKa = 10.85KK29 pKa = 10.02SGFVDD34 pKa = 3.99GTGKK38 pKa = 8.96TMLHH42 pKa = 5.14VHH44 pKa = 6.47VDD46 pKa = 3.81GQDD49 pKa = 3.16VTSALEE55 pKa = 3.51IAQAYY60 pKa = 8.89GGRR63 pKa = 11.84LTEE66 pKa = 4.55HH67 pKa = 6.56SEE69 pKa = 3.91AMPEE73 pKa = 3.67EE74 pKa = 4.76RR75 pKa = 11.84LYY77 pKa = 11.48QMAYY81 pKa = 10.63DD82 pKa = 3.98LDD84 pKa = 3.88EE85 pKa = 5.43GIRR88 pKa = 11.84IPAHH92 pKa = 5.81FVNEE96 pKa = 4.5DD97 pKa = 3.16FSEE100 pKa = 5.16SYY102 pKa = 9.65VQPGATEE109 pKa = 4.36AGGSPDD115 pKa = 4.21APAAPLYY122 pKa = 10.96DD123 pKa = 3.52EE124 pKa = 5.53FDD126 pKa = 4.16PSEE129 pKa = 3.9EE130 pKa = 4.49TYY132 pKa = 11.22DD133 pKa = 3.69GFSPGVHH140 pKa = 6.26LL141 pKa = 5.52

Molecular weight: 15.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C2RDH1|A0A0C2RDH1_9BACL N-6 DNA methylase OS=Paenibacillus sp. VKM B-2647 OX=1590651 GN=SD70_17860 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 8.77PTFNPNTRR10 pKa = 11.84KK11 pKa = 9.75RR12 pKa = 11.84KK13 pKa = 8.79KK14 pKa = 8.74VHH16 pKa = 5.61GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.78VLAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.23GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5046

0

5046

1449787

26

1921

287.3

31.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.365 ± 0.044

0.836 ± 0.009

5.054 ± 0.028

6.284 ± 0.042

4.192 ± 0.028

7.59 ± 0.033

2.115 ± 0.017

6.241 ± 0.031

5.136 ± 0.033

10.234 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.795 ± 0.016

3.449 ± 0.025

4.347 ± 0.022

3.738 ± 0.025

5.708 ± 0.032

5.815 ± 0.027

5.109 ± 0.026

7.282 ± 0.025

1.329 ± 0.014

3.383 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski