Pseudonocardia oroxyli

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Pseudonocardia

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5828 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G7LG41|A0A1G7LG41_9PSEU UPF0716 protein FxsA OS=Pseudonocardia oroxyli OX=366584 GN=SAMN05216377_10577 PE=4 SV=1
MM1 pKa = 7.47ARR3 pKa = 11.84TRR5 pKa = 11.84PGPNFGAIAEE15 pKa = 4.25RR16 pKa = 11.84VARR19 pKa = 11.84QPTPTHH25 pKa = 6.08SGMITMTLLDD35 pKa = 4.46FLLALLGDD43 pKa = 4.2SPEE46 pKa = 4.6DD47 pKa = 3.58LALQQDD53 pKa = 4.32FADD56 pKa = 4.15NPVATLQALDD66 pKa = 4.73LGDD69 pKa = 3.82LTAEE73 pKa = 4.42DD74 pKa = 3.54VHH76 pKa = 6.82DD77 pKa = 4.82ALVLVQDD84 pKa = 3.84NQTVEE89 pKa = 5.11FGDD92 pKa = 3.37TDD94 pKa = 4.37YY95 pKa = 10.43NTGGNTIVGGPVPAFAHH112 pKa = 6.47HH113 pKa = 5.93EE114 pKa = 4.05AAPEE118 pKa = 3.79GHH120 pKa = 6.22SAVEE124 pKa = 4.02YY125 pKa = 8.19IQTYY129 pKa = 6.03VTHH132 pKa = 6.96TEE134 pKa = 4.07VQDD137 pKa = 3.36GDD139 pKa = 4.2TVLGSPVQQVIDD151 pKa = 4.17AGDD154 pKa = 3.85DD155 pKa = 3.54VNQVITTTTTAASGAGAVAAQGDD178 pKa = 4.13IEE180 pKa = 4.7DD181 pKa = 4.17SQVVTGSGNVFGSGNVIGDD200 pKa = 4.2DD201 pKa = 3.36NTTAFGDD208 pKa = 3.89GDD210 pKa = 3.78ATSADD215 pKa = 3.42VHH217 pKa = 5.98GVSVSGGGAFSVTGPATGDD236 pKa = 3.4YY237 pKa = 11.17DD238 pKa = 3.94VTDD241 pKa = 3.74SGNTTTVTDD250 pKa = 3.62TTTTEE255 pKa = 4.04ISDD258 pKa = 3.78SGNTDD263 pKa = 2.49VDD265 pKa = 3.42SSYY268 pKa = 11.52SYY270 pKa = 11.28SQDD273 pKa = 3.13DD274 pKa = 3.57HH275 pKa = 9.06SEE277 pKa = 4.08TNTNVGNEE285 pKa = 4.1VHH287 pKa = 7.13DD288 pKa = 5.11GSHH291 pKa = 5.38NTSDD295 pKa = 4.93LEE297 pKa = 4.41INVV300 pKa = 3.63

Molecular weight: 30.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G7MR31|A0A1G7MR31_9PSEU GTPase G3E family OS=Pseudonocardia oroxyli OX=366584 GN=SAMN05216377_10621 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSSRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.64GRR40 pKa = 11.84AALSAA45 pKa = 3.96

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5828

0

5828

1837287

29

4794

315.3

33.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.212 ± 0.049

0.715 ± 0.008

6.055 ± 0.026

5.653 ± 0.032

2.658 ± 0.02

9.509 ± 0.033

2.132 ± 0.016

3.164 ± 0.024

1.532 ± 0.022

10.592 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.635 ± 0.013

1.503 ± 0.016

6.24 ± 0.031

2.544 ± 0.018

8.319 ± 0.034

4.773 ± 0.021

6.005 ± 0.03

9.507 ± 0.035

1.469 ± 0.014

1.783 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski