Tomato leaf curl Laos virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9QCV4|Q9QCV4_9GEMI Transcriptional activator protein OS=Tomato leaf curl Laos virus OX=223345 GN=C2 PE=3 SV=1
MM1 pKa = 7.35PRR3 pKa = 11.84AGRR6 pKa = 11.84FSIKK10 pKa = 10.18AKK12 pKa = 10.62NYY14 pKa = 8.86FLTYY18 pKa = 8.4PQCSLTKK25 pKa = 10.58EE26 pKa = 4.02EE27 pKa = 5.47ALSQLQTLQTPTNKK41 pKa = 10.07KK42 pKa = 8.99YY43 pKa = 10.85IKK45 pKa = 9.66ICRR48 pKa = 11.84EE49 pKa = 3.74LHH51 pKa = 6.25EE52 pKa = 5.87DD53 pKa = 4.22GSPHH57 pKa = 6.11LHH59 pKa = 6.16VLIQFEE65 pKa = 4.49GKK67 pKa = 10.13YY68 pKa = 10.33NCTNNRR74 pKa = 11.84FFDD77 pKa = 4.02LVSPTRR83 pKa = 11.84SAHH86 pKa = 4.86FHH88 pKa = 6.55PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.11VKK101 pKa = 11.17SYY103 pKa = 11.26LEE105 pKa = 4.24KK106 pKa = 11.19DD107 pKa = 3.24GDD109 pKa = 3.91TLDD112 pKa = 3.28WGEE115 pKa = 3.86FQIDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 4.37AYY134 pKa = 10.82AKK136 pKa = 10.41ALNSGSKK143 pKa = 10.2SEE145 pKa = 4.05ALNVIKK151 pKa = 10.37EE152 pKa = 4.29LAPKK156 pKa = 10.3DD157 pKa = 3.74YY158 pKa = 11.2VLQFHH163 pKa = 6.73NLNSNLDD170 pKa = 4.05RR171 pKa = 11.84IFAPPLEE178 pKa = 4.42VFVCPFLSSSFDD190 pKa = 3.44QVPEE194 pKa = 3.95EE195 pKa = 4.04LEE197 pKa = 3.96EE198 pKa = 4.16WVSEE202 pKa = 4.18NVRR205 pKa = 11.84DD206 pKa = 3.6AAARR210 pKa = 11.84PWRR213 pKa = 11.84PKK215 pKa = 10.37SIVIEE220 pKa = 4.41GEE222 pKa = 3.95SRR224 pKa = 11.84TGKK227 pKa = 8.58TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.15VYY251 pKa = 11.07SNDD254 pKa = 2.25AWYY257 pKa = 10.92NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.59YY287 pKa = 10.35GKK289 pKa = 9.51PMQIKK294 pKa = 10.29GGIPTIFLCNPGPTSSYY311 pKa = 11.03KK312 pKa = 10.55EE313 pKa = 3.94YY314 pKa = 10.94LDD316 pKa = 3.87EE317 pKa = 4.82EE318 pKa = 4.69KK319 pKa = 11.15NSALKK324 pKa = 10.57DD325 pKa = 3.44WAVKK329 pKa = 9.64NAEE332 pKa = 4.62FITLTEE338 pKa = 3.98PLYY341 pKa = 11.19SGTHH345 pKa = 4.78QSQAQNSQEE354 pKa = 4.09EE355 pKa = 4.74ANSQTT360 pKa = 3.52

Molecular weight: 40.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9QCV7|Q9QCV7_9GEMI Protein V2 OS=Tomato leaf curl Laos virus OX=223345 GN=V2 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.52IVISTPASKK16 pKa = 10.29VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.16TPYY27 pKa = 9.34TSRR30 pKa = 11.84AAVPTVLVTNKK41 pKa = 9.01RR42 pKa = 11.84RR43 pKa = 11.84AWIQRR48 pKa = 11.84PMYY51 pKa = 9.53RR52 pKa = 11.84KK53 pKa = 8.15PRR55 pKa = 11.84MYY57 pKa = 10.82RR58 pKa = 11.84MYY60 pKa = 10.57RR61 pKa = 11.84SPDD64 pKa = 3.29VPKK67 pKa = 10.81GCEE70 pKa = 4.39GPCKK74 pKa = 9.35VQSYY78 pKa = 6.87EE79 pKa = 3.74QRR81 pKa = 11.84HH82 pKa = 5.66DD83 pKa = 3.55VSHH86 pKa = 6.04VGKK89 pKa = 9.69VLCVSDD95 pKa = 3.49VTRR98 pKa = 11.84GNGLTHH104 pKa = 6.89RR105 pKa = 11.84VGKK108 pKa = 9.66RR109 pKa = 11.84FCVKK113 pKa = 9.94SVYY116 pKa = 10.52VLGKK120 pKa = 9.41IWMDD124 pKa = 3.35EE125 pKa = 4.04NIKK128 pKa = 9.32TKK130 pKa = 10.61NHH132 pKa = 6.03TNTVMFFLVRR142 pKa = 11.84DD143 pKa = 3.71RR144 pKa = 11.84RR145 pKa = 11.84PFGTAMDD152 pKa = 4.48FGQVFNMYY160 pKa = 10.66DD161 pKa = 3.86NEE163 pKa = 4.34PSTATVKK170 pKa = 10.93NDD172 pKa = 2.72MRR174 pKa = 11.84DD175 pKa = 3.1RR176 pKa = 11.84YY177 pKa = 9.86QVLRR181 pKa = 11.84KK182 pKa = 8.36FTATVTGGQYY192 pKa = 10.85ASKK195 pKa = 10.11EE196 pKa = 3.81QALVRR201 pKa = 11.84KK202 pKa = 9.47FMKK205 pKa = 9.77INNYY209 pKa = 8.31VVYY212 pKa = 10.35NHH214 pKa = 6.03QEE216 pKa = 3.42AAKK219 pKa = 10.4YY220 pKa = 9.25EE221 pKa = 4.13NHH223 pKa = 6.46TEE225 pKa = 3.99NALLLYY231 pKa = 7.29MACTHH236 pKa = 7.07ASNPVYY242 pKa = 9.86ATLKK246 pKa = 9.47IRR248 pKa = 11.84IYY250 pKa = 10.65FYY252 pKa = 11.33DD253 pKa = 3.43SVQNN257 pKa = 3.87

Molecular weight: 29.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1098

96

360

183.0

21.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.1 ± 0.762

2.459 ± 0.466

4.554 ± 0.38

4.918 ± 0.842

4.281 ± 0.546

4.645 ± 0.397

3.916 ± 0.637

4.918 ± 1.34

6.011 ± 0.673

7.104 ± 0.862

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.368 ± 0.642

5.829 ± 0.369

6.011 ± 0.605

5.191 ± 0.535

6.557 ± 1.155

7.923 ± 1.272

6.74 ± 0.807

5.738 ± 1.344

1.457 ± 0.246

4.281 ± 0.803
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski