Dyella sp. M7H15-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Rhodanobacteraceae; Dyella; unclassified Dyella

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2958 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A410UIR5|A0A410UIR5_9GAMM Protein TonB OS=Dyella sp. M7H15-1 OX=2501295 GN=EO087_08650 PE=3 SV=1
MM1 pKa = 7.18NQTSTDD7 pKa = 3.16TALRR11 pKa = 11.84KK12 pKa = 8.71WMCVVCGFIYY22 pKa = 10.61EE23 pKa = 4.29EE24 pKa = 4.11ALGLPEE30 pKa = 4.9EE31 pKa = 5.39GIDD34 pKa = 4.6PGTRR38 pKa = 11.84WSDD41 pKa = 3.73VPDD44 pKa = 2.97TWTCPDD50 pKa = 3.84CGATKK55 pKa = 10.7DD56 pKa = 3.88DD57 pKa = 4.33FEE59 pKa = 5.12MIEE62 pKa = 3.83IDD64 pKa = 3.43

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A410UKS2|A0A410UKS2_9GAMM Histidine kinase OS=Dyella sp. M7H15-1 OX=2501295 GN=EO087_13255 PE=4 SV=1
MM1 pKa = 6.48TRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 9.99GIPGLSFSWKK16 pKa = 8.18RR17 pKa = 11.84AVGISSAKK25 pKa = 10.39ANVSRR30 pKa = 11.84KK31 pKa = 9.58IGIPLTRR38 pKa = 11.84SGRR41 pKa = 11.84RR42 pKa = 11.84QRR44 pKa = 11.84MRR46 pKa = 11.84KK47 pKa = 9.57AKK49 pKa = 8.82GTLLTFIVGLFLVWMFVHH67 pKa = 7.17WLSQLL72 pKa = 3.39

Molecular weight: 8.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2958

0

2958

982631

30

3789

332.2

36.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.282 ± 0.06

0.93 ± 0.014

5.603 ± 0.034

5.076 ± 0.057

3.376 ± 0.029

8.096 ± 0.059

2.665 ± 0.024

4.818 ± 0.034

3.37 ± 0.043

10.5 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.468 ± 0.021

3.284 ± 0.047

5.013 ± 0.037

4.153 ± 0.029

6.513 ± 0.064

5.921 ± 0.046

5.573 ± 0.056

7.292 ± 0.039

1.438 ± 0.02

2.629 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski