Horseradish curly top virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Curtovirus

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q68541|Q68541_9GEMI Capsid protein OS=Horseradish curly top virus OX=46448 GN=V1 PE=3 SV=1
MM1 pKa = 7.49GPFTVGQFPRR11 pKa = 11.84NYY13 pKa = 8.54PALLAVSTSCFFRR26 pKa = 11.84YY27 pKa = 9.84NKK29 pKa = 9.27WCILGIRR36 pKa = 11.84HH37 pKa = 5.51EE38 pKa = 4.48VEE40 pKa = 4.03SLTLEE45 pKa = 4.04EE46 pKa = 5.28GEE48 pKa = 4.86AFLGFQKK55 pKa = 10.32EE56 pKa = 4.45VKK58 pKa = 10.09KK59 pKa = 10.69LLKK62 pKa = 10.24RR63 pKa = 11.84KK64 pKa = 8.4CTFSRR69 pKa = 11.84KK70 pKa = 9.13CEE72 pKa = 4.04LYY74 pKa = 9.89EE75 pKa = 4.31EE76 pKa = 4.8IYY78 pKa = 10.71KK79 pKa = 10.48KK80 pKa = 10.75YY81 pKa = 10.76LSDD84 pKa = 3.76GPEE87 pKa = 4.1EE88 pKa = 4.53KK89 pKa = 10.77GSLPTVMVEE98 pKa = 4.6EE99 pKa = 4.55EE100 pKa = 4.21EE101 pKa = 4.54DD102 pKa = 3.35WAHH105 pKa = 5.98EE106 pKa = 4.32KK107 pKa = 10.58IPMEE111 pKa = 4.2EE112 pKa = 4.12ACSQDD117 pKa = 3.38EE118 pKa = 4.41GPEE121 pKa = 3.98IEE123 pKa = 5.43DD124 pKa = 3.3VV125 pKa = 3.48

Molecular weight: 14.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q68542|Q68542_9GEMI C2 protein OS=Horseradish curly top virus OX=46448 GN=C2 PE=4 SV=1
MM1 pKa = 7.77RR2 pKa = 11.84RR3 pKa = 11.84YY4 pKa = 7.95TRR6 pKa = 11.84NTYY9 pKa = 10.93QMGQKK14 pKa = 9.85RR15 pKa = 11.84KK16 pKa = 10.17APYY19 pKa = 8.95QPSWSKK25 pKa = 10.73KK26 pKa = 8.69RR27 pKa = 11.84KK28 pKa = 6.67TGPMRR33 pKa = 11.84KK34 pKa = 8.84YY35 pKa = 8.51QWKK38 pKa = 9.82RR39 pKa = 11.84PARR42 pKa = 11.84KK43 pKa = 7.72TKK45 pKa = 9.95ALKK48 pKa = 10.69LKK50 pKa = 9.95MYY52 pKa = 10.75DD53 pKa = 3.79DD54 pKa = 4.03MLGAGGLGSTISNNGMITMLNNYY77 pKa = 7.83VQGIGDD83 pKa = 3.98SQRR86 pKa = 11.84STNTTITKK94 pKa = 10.06HH95 pKa = 5.48MKK97 pKa = 10.06FDD99 pKa = 3.53MALMGSSSFWDD110 pKa = 3.75TPNYY114 pKa = 5.22MTQYY118 pKa = 10.02HH119 pKa = 7.06WIIIDD124 pKa = 3.58KK125 pKa = 10.67DD126 pKa = 3.73VGSVFPVKK134 pKa = 10.53LDD136 pKa = 4.49DD137 pKa = 4.96IFDD140 pKa = 3.95IPSNGQAMPSTFRR153 pKa = 11.84IRR155 pKa = 11.84RR156 pKa = 11.84DD157 pKa = 3.21SNEE160 pKa = 3.46RR161 pKa = 11.84FIVKK165 pKa = 10.08RR166 pKa = 11.84KK167 pKa = 4.84WTTHH171 pKa = 4.9LMSTGAAYY179 pKa = 10.06GSKK182 pKa = 10.3DD183 pKa = 3.15IYY185 pKa = 10.6KK186 pKa = 10.06GPSMPNYY193 pKa = 9.47KK194 pKa = 10.07KK195 pKa = 11.03GMNINIRR202 pKa = 11.84NLNVKK207 pKa = 7.51TIWKK211 pKa = 7.76DD212 pKa = 3.19TGGGKK217 pKa = 10.11YY218 pKa = 10.33EE219 pKa = 4.14DD220 pKa = 3.64VKK222 pKa = 11.32EE223 pKa = 3.86NAILYY228 pKa = 9.99IVVNDD233 pKa = 3.8NADD236 pKa = 3.72NTNMYY241 pKa = 8.16ATLFGNCRR249 pKa = 11.84CYY251 pKa = 10.31FYY253 pKa = 11.55

Molecular weight: 29.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1067

88

346

177.8

20.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.217 ± 0.868

1.874 ± 0.447

5.155 ± 0.73

5.436 ± 1.933

4.592 ± 0.601

5.998 ± 0.754

2.249 ± 0.629

6.654 ± 0.933

6.935 ± 0.957

7.216 ± 1.107

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.093 ± 0.89

5.53 ± 1.174

5.717 ± 0.972

3.749 ± 0.438

5.53 ± 0.461

7.404 ± 1.33

7.31 ± 1.308

5.061 ± 0.558

1.781 ± 0.313

4.499 ± 0.656
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski