Pacific flying fox faeces associated circular DNA virus-3

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 9.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140CTT1|A0A140CTT1_9VIRU Putative capsid protein OS=Pacific flying fox faeces associated circular DNA virus-3 OX=1796012 PE=4 SV=1
MM1 pKa = 8.01PKK3 pKa = 10.22RR4 pKa = 11.84IRR6 pKa = 11.84QPRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84WVFTLNNFTEE22 pKa = 4.46EE23 pKa = 4.06EE24 pKa = 4.24LTTIKK29 pKa = 10.92NILTDD34 pKa = 4.24LAPTYY39 pKa = 10.6AIIGKK44 pKa = 8.81EE45 pKa = 3.95VGEE48 pKa = 4.93CGTPHH53 pKa = 6.16LQGFVSLKK61 pKa = 9.95KK62 pKa = 10.37QISFTAMKK70 pKa = 10.4SLIGTRR76 pKa = 11.84AHH78 pKa = 6.77IEE80 pKa = 3.95AANGTDD86 pKa = 3.33VDD88 pKa = 3.91NQLYY92 pKa = 10.28CSKK95 pKa = 10.6QDD97 pKa = 3.13ADD99 pKa = 3.24AWQLGEE105 pKa = 4.24PRR107 pKa = 11.84QSVTGRR113 pKa = 11.84GGNALDD119 pKa = 3.59YY120 pKa = 10.95RR121 pKa = 11.84GVLEE125 pKa = 4.4ALQSGSTVTEE135 pKa = 3.86IVKK138 pKa = 10.72NPDD141 pKa = 3.1LVVPLIIQHH150 pKa = 6.23RR151 pKa = 11.84NLRR154 pKa = 11.84QVLLDD159 pKa = 3.71LKK161 pKa = 10.85KK162 pKa = 10.76EE163 pKa = 4.01KK164 pKa = 10.27EE165 pKa = 4.14LEE167 pKa = 4.13KK168 pKa = 11.08LRR170 pKa = 11.84TAYY173 pKa = 10.0KK174 pKa = 10.5NVTLRR179 pKa = 11.84PFQEE183 pKa = 3.95EE184 pKa = 4.36LVNILSSDD192 pKa = 3.52PDD194 pKa = 3.32SRR196 pKa = 11.84AVYY199 pKa = 9.21WYY201 pKa = 9.78YY202 pKa = 11.12DD203 pKa = 3.25QYY205 pKa = 11.9GNTGKK210 pKa = 7.83TWFSKK215 pKa = 10.71YY216 pKa = 9.92LVARR220 pKa = 11.84MDD222 pKa = 3.78ALRR225 pKa = 11.84LEE227 pKa = 4.41NGKK230 pKa = 7.79TADD233 pKa = 3.98LKK235 pKa = 10.89HH236 pKa = 6.98AYY238 pKa = 8.69EE239 pKa = 4.26GQKK242 pKa = 9.96IVIFDD247 pKa = 3.88FSRR250 pKa = 11.84STMEE254 pKa = 4.49RR255 pKa = 11.84INYY258 pKa = 8.31EE259 pKa = 4.05CIEE262 pKa = 4.48AIKK265 pKa = 10.77NGLVFSGKK273 pKa = 10.13YY274 pKa = 9.66EE275 pKa = 4.09STSKK279 pKa = 10.31IFPVPHH285 pKa = 5.47VVCFANCPPDD295 pKa = 3.79TSKK298 pKa = 11.18LSYY301 pKa = 10.38DD302 pKa = 3.13RR303 pKa = 11.84WRR305 pKa = 11.84IRR307 pKa = 11.84CIDD310 pKa = 4.26EE311 pKa = 3.97INISVGGTYY320 pKa = 8.22QMRR323 pKa = 11.84WVRR326 pKa = 11.84GGSRR330 pKa = 11.84LRR332 pKa = 11.84RR333 pKa = 11.84SRR335 pKa = 11.84PAFLII340 pKa = 4.09

Molecular weight: 38.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140CTT1|A0A140CTT1_9VIRU Putative capsid protein OS=Pacific flying fox faeces associated circular DNA virus-3 OX=1796012 PE=4 SV=1
MM1 pKa = 7.59VKK3 pKa = 10.13RR4 pKa = 11.84QSTLASWRR12 pKa = 11.84RR13 pKa = 11.84VKK15 pKa = 10.19RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84MVRR21 pKa = 11.84SRR23 pKa = 11.84RR24 pKa = 11.84VRR26 pKa = 11.84SRR28 pKa = 11.84GRR30 pKa = 11.84SVLYY34 pKa = 9.77RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84TFRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84FVRR47 pKa = 11.84RR48 pKa = 11.84NKK50 pKa = 9.95YY51 pKa = 10.36LSGIYY56 pKa = 10.22AQFTTPIKK64 pKa = 10.24WSDD67 pKa = 3.01IGDD70 pKa = 3.95DD71 pKa = 4.49GFATMNLNLNIAAMIGDD88 pKa = 4.55IQPQLTGYY96 pKa = 10.27RR97 pKa = 11.84VVFDD101 pKa = 3.37QAKK104 pKa = 8.43VLRR107 pKa = 11.84IKK109 pKa = 10.12RR110 pKa = 11.84TVWLNDD116 pKa = 2.81SDD118 pKa = 5.0EE119 pKa = 4.23YY120 pKa = 11.21TKK122 pKa = 11.1ADD124 pKa = 3.43QRR126 pKa = 11.84VTEE129 pKa = 5.27LYY131 pKa = 10.65SSYY134 pKa = 11.47DD135 pKa = 3.25PDD137 pKa = 4.69ANGSSLTQAQIIQDD151 pKa = 3.59PSHH154 pKa = 7.24RR155 pKa = 11.84MVIMKK160 pKa = 9.1PFSRR164 pKa = 11.84YY165 pKa = 9.65RR166 pKa = 11.84YY167 pKa = 7.94WIKK170 pKa = 10.68PNWRR174 pKa = 11.84LQNATQGTPALFSYY188 pKa = 9.64TGYY191 pKa = 11.27NDD193 pKa = 3.37ILDD196 pKa = 3.86WQADD200 pKa = 4.0RR201 pKa = 11.84TGNSANSVHH210 pKa = 5.43MVARR214 pKa = 11.84GTTEE218 pKa = 3.36QGAINYY224 pKa = 8.93QDD226 pKa = 2.91TYY228 pKa = 11.14YY229 pKa = 10.35IHH231 pKa = 6.62FKK233 pKa = 10.84KK234 pKa = 10.46RR235 pKa = 11.84IQNAGGTT242 pKa = 3.92

Molecular weight: 28.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

582

242

340

291.0

33.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.014 ± 0.364

1.031 ± 0.628

5.498 ± 0.427

4.124 ± 1.758

3.436 ± 0.08

6.186 ± 0.244

1.375 ± 0.082

6.529 ± 0.202

5.67 ± 0.685

7.56 ± 1.334

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.062 ± 0.506

4.983 ± 0.237

3.608 ± 0.436

4.983 ± 0.741

10.481 ± 1.923

6.529 ± 0.302

6.873 ± 0.344

6.186 ± 0.244

2.062 ± 0.254

4.811 ± 0.594
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski