Escherichia virus Jk06

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Rogunavirinae; Rogunavirus

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q45PW9|Q45PW9_9CAUD JK_47P OS=Escherichia virus Jk06 OX=1920998 GN=JK_47 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 10.38AIIINNEE9 pKa = 2.81IRR11 pKa = 11.84YY12 pKa = 9.99DD13 pKa = 4.01EE14 pKa = 5.36DD15 pKa = 4.13SMSLAEE21 pKa = 4.91LGYY24 pKa = 10.56QIGEE28 pKa = 4.03EE29 pKa = 4.26VEE31 pKa = 4.27VYY33 pKa = 10.14EE34 pKa = 4.9IEE36 pKa = 4.43GFYY39 pKa = 10.58MMSLKK44 pKa = 10.84DD45 pKa = 3.5HH46 pKa = 6.56VYY48 pKa = 10.84VDD50 pKa = 3.43GTLAIFQGEE59 pKa = 4.15QFVVSNEE66 pKa = 4.09EE67 pKa = 3.88IKK69 pKa = 11.1VVAA72 pKa = 4.56

Molecular weight:
8.26 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q45PX8|Q45PX8_9CAUD JK_38P OS=Escherichia virus Jk06 OX=1920998 GN=JK_38 PE=4 SV=1
MM1 pKa = 7.08KK2 pKa = 9.74TLVWEE7 pKa = 4.39EE8 pKa = 3.71MTPAEE13 pKa = 4.17KK14 pKa = 10.56LAVKK18 pKa = 10.24ALSTHH23 pKa = 5.25SFEE26 pKa = 4.52AFLRR30 pKa = 11.84VWFQLSQGEE39 pKa = 4.17RR40 pKa = 11.84YY41 pKa = 9.13IPNWHH46 pKa = 6.78HH47 pKa = 7.09KK48 pKa = 7.75YY49 pKa = 9.91LCRR52 pKa = 11.84IIDD55 pKa = 3.65EE56 pKa = 4.72VISGEE61 pKa = 4.42RR62 pKa = 11.84RR63 pKa = 11.84DD64 pKa = 3.98TIINVAPGAGKK75 pKa = 8.19TEE77 pKa = 3.77ITSIHH82 pKa = 5.92FPVYY86 pKa = 11.26SMLKK90 pKa = 9.37LKK92 pKa = 10.38KK93 pKa = 10.06VRR95 pKa = 11.84NLSLSFSDD103 pKa = 4.35SLVKK107 pKa = 10.62RR108 pKa = 11.84NSKK111 pKa = 9.98RR112 pKa = 11.84VRR114 pKa = 11.84DD115 pKa = 4.13LIKK118 pKa = 10.55SVSFRR123 pKa = 11.84NYY125 pKa = 10.16GPVSSARR132 pKa = 11.84ARR134 pKa = 11.84MTRR137 pKa = 11.84FRR139 pKa = 11.84FLTRR143 pKa = 11.84VEE145 pKa = 3.93RR146 pKa = 11.84LGSKK150 pKa = 10.2RR151 pKa = 11.84YY152 pKa = 9.61LRR154 pKa = 11.84QLAVRR159 pKa = 11.84LPALVAVILLKK170 pKa = 10.67PIAVAA175 pKa = 4.34

Molecular weight:
20.2 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

14581

72

1141

177.8

19.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.079 ± 0.371

1.536 ± 0.173

5.596 ± 0.252

5.74 ± 0.325

3.985 ± 0.172

6.913 ± 0.329

1.598 ± 0.164

6.392 ± 0.161

5.706 ± 0.366

8.497 ± 0.353

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.592 ± 0.185

5.013 ± 0.257

3.319 ± 0.213

3.854 ± 0.21

5.473 ± 0.252

7.976 ± 0.362

5.974 ± 0.265

7.091 ± 0.269

1.125 ± 0.089

3.539 ± 0.185

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski