Culex negev-like virus 1

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z2RTJ3|A0A1Z2RTJ3_9VIRU Uncharacterized protein OS=Culex negev-like virus 1 OX=2010273 PE=4 SV=1
MM1 pKa = 7.08FALFVFVVALCSLPADD17 pKa = 3.49SHH19 pKa = 6.68VYY21 pKa = 5.76PTPVFRR27 pKa = 11.84FSDD30 pKa = 3.79CHH32 pKa = 6.1NSSLFFRR39 pKa = 11.84NNSNAHH45 pKa = 5.09VADD48 pKa = 4.0LEE50 pKa = 4.5VRR52 pKa = 11.84CDD54 pKa = 3.81IVEE57 pKa = 4.38LPCSVISNRR66 pKa = 11.84QATYY70 pKa = 6.26MTLRR74 pKa = 11.84FDD76 pKa = 4.33EE77 pKa = 5.28FPSNCTYY84 pKa = 9.62ITRR87 pKa = 11.84KK88 pKa = 10.33DD89 pKa = 3.74NPCVFTPPCAKK100 pKa = 9.94FRR102 pKa = 11.84ILQIHH107 pKa = 6.66DD108 pKa = 3.65AFHH111 pKa = 6.34NVDD114 pKa = 4.05TIVCIGVLKK123 pKa = 9.6DD124 pKa = 3.45TQFPPIRR131 pKa = 11.84HH132 pKa = 5.91AKK134 pKa = 7.99VAPVQQYY141 pKa = 8.58FSNMTTFLYY150 pKa = 10.62ASKK153 pKa = 10.94NSVKK157 pKa = 10.28FSLYY161 pKa = 8.6DD162 pKa = 3.36TPQIRR167 pKa = 11.84NLNIYY172 pKa = 9.24ALCFDD177 pKa = 4.77DD178 pKa = 4.07EE179 pKa = 5.21AYY181 pKa = 10.79AKK183 pKa = 9.25FIPKK187 pKa = 10.24ALLDD191 pKa = 3.64QMNQSSLLSDD201 pKa = 3.22QFVTVDD207 pKa = 3.26TSTEE211 pKa = 3.81FTQIFLNYY219 pKa = 8.5HH220 pKa = 5.53PNMRR224 pKa = 11.84KK225 pKa = 9.47YY226 pKa = 10.74LDD228 pKa = 3.89SNVFPLPLAVPQGYY242 pKa = 8.72NLQTSCNSSAPVYY255 pKa = 9.25QQIVPAADD263 pKa = 3.03KK264 pKa = 10.26MIYY267 pKa = 9.78PIVNGYY273 pKa = 10.37DD274 pKa = 3.59DD275 pKa = 4.01STCRR279 pKa = 11.84DD280 pKa = 3.71PLIIPPYY287 pKa = 8.86LYY289 pKa = 11.23CMDD292 pKa = 3.69YY293 pKa = 10.51FYY295 pKa = 11.43GRR297 pKa = 11.84GRR299 pKa = 11.84SCPLKK304 pKa = 10.78VNPPSIAGAPNFHH317 pKa = 6.79MFTRR321 pKa = 11.84WYY323 pKa = 9.74IRR325 pKa = 11.84SPP327 pKa = 3.31

Molecular weight: 37.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z2RTJ3|A0A1Z2RTJ3_9VIRU Uncharacterized protein OS=Culex negev-like virus 1 OX=2010273 PE=4 SV=1
MM1 pKa = 7.72AIIDD5 pKa = 4.77EE6 pKa = 4.72IDD8 pKa = 3.18TTTNNRR14 pKa = 11.84EE15 pKa = 3.89RR16 pKa = 11.84RR17 pKa = 11.84LTTQGVTVIRR27 pKa = 11.84GGQTVNDD34 pKa = 3.96SFFQATLSCYY44 pKa = 10.96ANIVYY49 pKa = 9.98HH50 pKa = 6.63PIAMYY55 pKa = 9.71MLALAIMFALSEE67 pKa = 4.1HH68 pKa = 6.8HH69 pKa = 6.56GTDD72 pKa = 3.72GPLEE76 pKa = 4.17VIKK79 pKa = 11.12NIFVKK84 pKa = 10.6FADD87 pKa = 4.44DD88 pKa = 4.41DD89 pKa = 4.24KK90 pKa = 11.84EE91 pKa = 4.28PDD93 pKa = 3.69ALRR96 pKa = 11.84KK97 pKa = 7.87IAKK100 pKa = 9.97YY101 pKa = 8.72MITVFTHH108 pKa = 7.02LITYY112 pKa = 8.47KK113 pKa = 10.66DD114 pKa = 3.42KK115 pKa = 10.46YY116 pKa = 8.98ATLIFISVAPIAKK129 pKa = 9.59PSTRR133 pKa = 11.84NIIFASLITISLILGSFTLLEE154 pKa = 4.11MFIFSQLFYY163 pKa = 10.57LHH165 pKa = 6.52VMYY168 pKa = 10.84RR169 pKa = 11.84NPTYY173 pKa = 11.07KK174 pKa = 10.65LLAVFAMVGLFFLDD188 pKa = 3.61NFFTPSTPEE197 pKa = 3.83SPPTQTTTSKK207 pKa = 10.87PPITTTTKK215 pKa = 8.8TSRR218 pKa = 11.84LRR220 pKa = 11.84RR221 pKa = 11.84SVGDD225 pKa = 3.85AFSHH229 pKa = 6.1TSDD232 pKa = 3.37PTVV235 pKa = 2.74

Molecular weight: 26.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3288

235

2726

1096.0

125.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.326 ± 0.249

2.129 ± 0.635

7.238 ± 0.71

4.106 ± 1.049

5.049 ± 1.322

3.802 ± 0.736

2.676 ± 0.097

6.539 ± 0.678

6.63 ± 1.415

8.029 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.768 ± 0.161

4.775 ± 0.555

4.532 ± 1.532

3.224 ± 0.374

4.988 ± 0.275

6.813 ± 0.292

7.451 ± 1.288

6.995 ± 0.352

0.73 ± 0.281

5.201 ± 0.339
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski