Pseudomonas phage YMC12/01/R24

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Casadabanvirus; unclassified Casadabanvirus

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346FB95|A0A346FB95_9CAUD Uncharacterized protein OS=Pseudomonas phage YMC12/01/R24 OX=2283028 GN=PAER24_00530 PE=4 SV=1
MM1 pKa = 7.46SFNSRR6 pKa = 11.84EE7 pKa = 3.86SSLADD12 pKa = 3.51GQPVRR17 pKa = 11.84LYY19 pKa = 10.17QFSRR23 pKa = 11.84GAIRR27 pKa = 11.84WSYY30 pKa = 10.82NSSDD34 pKa = 4.7RR35 pKa = 11.84DD36 pKa = 3.12ITYY39 pKa = 10.21QNQIFRR45 pKa = 11.84TVPGGITDD53 pKa = 3.54NGIICSGDD61 pKa = 3.43PQSDD65 pKa = 3.53QFVITAPADD74 pKa = 3.6LDD76 pKa = 3.68VALLYY81 pKa = 8.52KK82 pKa = 10.05TRR84 pKa = 11.84SPSGAIDD91 pKa = 3.52LVVYY95 pKa = 10.5DD96 pKa = 3.72MHH98 pKa = 8.34YY99 pKa = 11.14GDD101 pKa = 4.46TEE103 pKa = 4.2AAVSWVGQIGDD114 pKa = 4.0VDD116 pKa = 3.86WPTVDD121 pKa = 3.06SCRR124 pKa = 11.84ITCVSEE130 pKa = 4.47DD131 pKa = 3.9EE132 pKa = 6.08LMDD135 pKa = 3.76QPGLIDD141 pKa = 3.86TYY143 pKa = 11.23CRR145 pKa = 11.84TCTAVVGDD153 pKa = 4.48HH154 pKa = 6.1RR155 pKa = 11.84CKK157 pKa = 11.04VNLVPYY163 pKa = 9.97RR164 pKa = 11.84VTLTPQSISGWVISSGVVAGYY185 pKa = 10.36VDD187 pKa = 3.51GWFTGGYY194 pKa = 8.66VEE196 pKa = 4.37WQVDD200 pKa = 3.02GDD202 pKa = 4.29NYY204 pKa = 10.6DD205 pKa = 2.73SRR207 pKa = 11.84YY208 pKa = 9.2IEE210 pKa = 4.13RR211 pKa = 11.84HH212 pKa = 5.85AGPDD216 pKa = 3.48LYY218 pKa = 10.62ILGGTEE224 pKa = 5.08GIPAGGQLRR233 pKa = 11.84VYY235 pKa = 9.42PGCDD239 pKa = 3.1GLAQTCDD246 pKa = 3.63DD247 pKa = 4.57KK248 pKa = 11.59FSNLPNFRR256 pKa = 11.84GFNAMQGKK264 pKa = 9.57SPFDD268 pKa = 4.06GDD270 pKa = 4.13QVWW273 pKa = 3.01

Molecular weight: 29.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346FB60|A0A346FB60_9CAUD Uncharacterized protein OS=Pseudomonas phage YMC12/01/R24 OX=2283028 GN=PAER24_00180 PE=4 SV=1
MM1 pKa = 7.2TNEE4 pKa = 4.2TKK6 pKa = 10.15TDD8 pKa = 3.35RR9 pKa = 11.84QRR11 pKa = 11.84RR12 pKa = 11.84LARR15 pKa = 11.84EE16 pKa = 3.61RR17 pKa = 11.84QRR19 pKa = 11.84AKK21 pKa = 10.65RR22 pKa = 11.84EE23 pKa = 3.43RR24 pKa = 11.84DD25 pKa = 3.22ALRR28 pKa = 11.84RR29 pKa = 11.84AALGGRR35 pKa = 11.84RR36 pKa = 11.84FNMDD40 pKa = 3.83MYY42 pKa = 10.99QGTADD47 pKa = 4.65ALDD50 pKa = 5.18LICAAGGFAEE60 pKa = 4.46PAEE63 pKa = 4.31AVTLLLHH70 pKa = 6.04NVAEE74 pKa = 4.23IAEE77 pKa = 4.21RR78 pKa = 11.84DD79 pKa = 3.33ASRR82 pKa = 11.84FAEE85 pKa = 4.92LIQKK89 pKa = 9.95RR90 pKa = 11.84SHH92 pKa = 6.71PGRR95 pKa = 11.84TKK97 pKa = 10.55RR98 pKa = 4.06

Molecular weight: 11.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

14607

38

1188

208.7

22.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.295 ± 0.496

0.883 ± 0.12

5.682 ± 0.21

5.983 ± 0.258

2.807 ± 0.169

7.921 ± 0.281

1.739 ± 0.156

4.19 ± 0.198

3.389 ± 0.271

10.043 ± 0.441

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.266 ± 0.121

2.779 ± 0.209

5.285 ± 0.278

5.237 ± 0.333

7.483 ± 0.324

5.792 ± 0.242

5.176 ± 0.271

6.723 ± 0.246

1.643 ± 0.122

2.684 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski