[Clostridium] symbiosum WAL-14163

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnoclostridium; [Clostridium] symbiosum

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4668 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E7GNS1|E7GNS1_CLOSY Uncharacterized protein OS=[Clostridium] symbiosum WAL-14163 OX=742740 GN=HMPREF9474_02566 PE=4 SV=1
MM1 pKa = 7.43AASLTACSGDD11 pKa = 3.38SGTSGSGSTAAPAPAAEE28 pKa = 4.35SQGGEE33 pKa = 4.37GEE35 pKa = 4.68GVQAPSVDD43 pKa = 2.95IDD45 pKa = 3.47RR46 pKa = 11.84ATEE49 pKa = 3.96FVTIATGPTSGIYY62 pKa = 10.2YY63 pKa = 9.29PIGGAFATVLGNAGYY78 pKa = 7.86KK79 pKa = 9.67TSAQATGASVEE90 pKa = 4.22NINLITAGEE99 pKa = 4.18AEE101 pKa = 4.81LAIAMQDD108 pKa = 3.16SVMQAYY114 pKa = 9.75EE115 pKa = 4.03GFGAFEE121 pKa = 4.26TPNTEE126 pKa = 2.93LRR128 pKa = 11.84AAMRR132 pKa = 11.84LWPNYY137 pKa = 8.35VQLVTTANTGIKK149 pKa = 10.07SVEE152 pKa = 3.95DD153 pKa = 3.47LKK155 pKa = 11.38GKK157 pKa = 10.07RR158 pKa = 11.84VGVGAPNSGVEE169 pKa = 3.94LNARR173 pKa = 11.84MIYY176 pKa = 9.41EE177 pKa = 4.71AYY179 pKa = 10.32GMTYY183 pKa = 10.22EE184 pKa = 4.73DD185 pKa = 4.53SEE187 pKa = 4.43VDD189 pKa = 3.39YY190 pKa = 11.15LSYY193 pKa = 11.54GEE195 pKa = 6.07AIDD198 pKa = 4.03QMKK201 pKa = 10.68NGQCDD206 pKa = 3.21AAFVTSGLPNSTVSEE221 pKa = 4.16LAFSYY226 pKa = 11.55DD227 pKa = 3.58MVIVPIDD234 pKa = 3.4GDD236 pKa = 3.86GRR238 pKa = 11.84DD239 pKa = 3.75NLINKK244 pKa = 9.0YY245 pKa = 9.91PFFAASIIPADD256 pKa = 3.77TYY258 pKa = 11.75NNEE261 pKa = 3.86EE262 pKa = 4.08DD263 pKa = 3.83VEE265 pKa = 4.97SVFVYY270 pKa = 11.07NIMLVNKK277 pKa = 9.78DD278 pKa = 3.8LSNDD282 pKa = 3.25MVYY285 pKa = 11.64DD286 pKa = 3.6MMDD289 pKa = 4.37CIFNDD294 pKa = 2.96IGSIKK299 pKa = 10.25ASHH302 pKa = 5.88NTADD306 pKa = 3.49QNIDD310 pKa = 3.21VTFGVDD316 pKa = 3.25DD317 pKa = 4.1VKK319 pKa = 11.17IPLHH323 pKa = 6.94DD324 pKa = 4.75GAAKK328 pKa = 8.58WWQDD332 pKa = 2.8HH333 pKa = 7.02GYY335 pKa = 7.78DD336 pKa = 3.7TPQNN340 pKa = 3.62

Molecular weight: 36.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E7GSD3|E7GSD3_CLOSY SpoJ protein OS=[Clostridium] symbiosum WAL-14163 OX=742740 GN=HMPREF9474_03828 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.79KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.44VHH16 pKa = 5.57GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSAGGRR28 pKa = 11.84KK29 pKa = 8.81VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.29GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 4.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4668

0

4668

1494359

31

4534

320.1

35.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.07 ± 0.041

1.511 ± 0.015

5.338 ± 0.028

7.555 ± 0.043

4.13 ± 0.029

7.756 ± 0.036

1.625 ± 0.013

7.098 ± 0.038

6.282 ± 0.029

9.142 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.168 ± 0.023

4.0 ± 0.025

3.585 ± 0.021

3.074 ± 0.023

4.893 ± 0.03

5.855 ± 0.027

5.347 ± 0.031

6.7 ± 0.03

0.941 ± 0.012

3.931 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski