Streptomyces sp. AA4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8394 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|D9UXG6|D9UXG6_9ACTN Tryptophan--tRNA ligase OS=Streptomyces sp. AA4 OX=591158 GN=SSMG_06442 PE=3 SV=1
MM1 pKa = 7.58SGTRR5 pKa = 11.84PQHH8 pKa = 6.84PNDD11 pKa = 2.95AWYY14 pKa = 9.99GGTMAITLDD23 pKa = 3.61AQLLEE28 pKa = 4.38ILACPSPDD36 pKa = 3.39HH37 pKa = 6.95APLRR41 pKa = 11.84PGTPDD46 pKa = 3.56DD47 pKa = 4.71AEE49 pKa = 5.91ADD51 pKa = 3.36ALTCTEE57 pKa = 4.44CGRR60 pKa = 11.84VYY62 pKa = 10.34PVRR65 pKa = 11.84DD66 pKa = 4.78GIPVLLLDD74 pKa = 4.22EE75 pKa = 4.57ATLPGTAGPDD85 pKa = 3.4ADD87 pKa = 4.93HH88 pKa = 7.24GDD90 pKa = 3.86SAA92 pKa = 5.04

Molecular weight:
9.56 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|D9VIX6|D9VIX6_9ACTN HTH cro/C1-type domain-containing protein OS=Streptomyces sp. AA4 OX=591158 GN=SSMG_08157 PE=4 SV=1
MM1 pKa = 7.94AGMGLDD7 pKa = 3.15RR8 pKa = 11.84GLHH11 pKa = 5.22RR12 pKa = 11.84RR13 pKa = 11.84ASRR16 pKa = 11.84GPRR19 pKa = 11.84HH20 pKa = 6.04RR21 pKa = 11.84PSRR24 pKa = 11.84PQEE27 pKa = 4.11DD28 pKa = 3.11PGARR32 pKa = 11.84GRR34 pKa = 11.84GTRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84PGRR42 pKa = 11.84RR43 pKa = 11.84EE44 pKa = 3.57TLRR47 pKa = 11.84PRR49 pKa = 11.84SQARR53 pKa = 11.84VQARR57 pKa = 11.84RR58 pKa = 11.84LRR60 pKa = 11.84EE61 pKa = 3.57GSRR64 pKa = 11.84PDD66 pKa = 2.78RR67 pKa = 11.84HH68 pKa = 5.78GRR70 pKa = 11.84NRR72 pKa = 11.84LPRR75 pKa = 11.84PAARR79 pKa = 11.84VAAAPAAGRR88 pKa = 11.84GRR90 pKa = 11.84PRR92 pKa = 11.84PPRR95 pKa = 11.84GSRR98 pKa = 11.84TRR100 pKa = 11.84RR101 pKa = 11.84RR102 pKa = 11.84RR103 pKa = 11.84PRR105 pKa = 11.84RR106 pKa = 11.84ARR108 pKa = 11.84RR109 pKa = 11.84ARR111 pKa = 11.84RR112 pKa = 11.84RR113 pKa = 11.84SRR115 pKa = 11.84DD116 pKa = 3.5RR117 pKa = 11.84GPGAADD123 pKa = 3.52PPHH126 pKa = 6.78RR127 pKa = 11.84RR128 pKa = 11.84ARR130 pKa = 11.84PARR133 pKa = 11.84RR134 pKa = 11.84GQRR137 pKa = 11.84RR138 pKa = 11.84RR139 pKa = 11.84RR140 pKa = 11.84GLVRR144 pKa = 11.84PAARR148 pKa = 11.84RR149 pKa = 11.84ARR151 pKa = 11.84CGAGVPAQPSRR162 pKa = 11.84RR163 pKa = 11.84PGAGRR168 pKa = 11.84PRR170 pKa = 11.84PGPRR174 pKa = 11.84RR175 pKa = 11.84RR176 pKa = 11.84RR177 pKa = 11.84PRR179 pKa = 11.84EE180 pKa = 3.33ADD182 pKa = 2.88HH183 pKa = 7.27RR184 pKa = 11.84IRR186 pKa = 11.84PGPLGRR192 pKa = 11.84PLLAPARR199 pKa = 11.84RR200 pKa = 11.84RR201 pKa = 11.84IRR203 pKa = 11.84RR204 pKa = 11.84AGPDD208 pKa = 2.89RR209 pKa = 11.84RR210 pKa = 11.84RR211 pKa = 11.84GRR213 pKa = 11.84DD214 pKa = 3.32PRR216 pKa = 11.84EE217 pKa = 3.77DD218 pKa = 3.74RR219 pKa = 11.84EE220 pKa = 4.16PGRR223 pKa = 11.84PVPAPDD229 pKa = 2.91RR230 pKa = 11.84VRR232 pKa = 11.84RR233 pKa = 11.84PRR235 pKa = 11.84RR236 pKa = 11.84ARR238 pKa = 11.84PDD240 pKa = 3.29RR241 pKa = 11.84GIHH244 pKa = 5.93RR245 pKa = 11.84PHH247 pKa = 6.69AALRR251 pKa = 11.84RR252 pKa = 11.84HH253 pKa = 5.58PRR255 pKa = 11.84TRR257 pKa = 11.84RR258 pKa = 11.84ADD260 pKa = 3.53PQIPQHH266 pKa = 5.4PQHH269 pKa = 6.64RR270 pKa = 11.84PVRR273 pKa = 11.84QEE275 pKa = 3.31PAAAGAARR283 pKa = 11.84AARR286 pKa = 11.84SRR288 pKa = 11.84RR289 pKa = 11.84ARR291 pKa = 11.84QPGVRR296 pKa = 11.84GGPVRR301 pKa = 11.84RR302 pKa = 11.84GRR304 pKa = 11.84ADD306 pKa = 3.39GPRR309 pKa = 11.84PAGAADD315 pKa = 3.88DD316 pKa = 4.13VPRR319 pKa = 11.84DD320 pKa = 3.44LRR322 pKa = 11.84DD323 pKa = 3.27RR324 pKa = 11.84ADD326 pKa = 3.74RR327 pKa = 11.84QPPRR331 pKa = 11.84RR332 pKa = 11.84GRR334 pKa = 11.84RR335 pKa = 11.84AGAGRR340 pKa = 11.84AAPRR344 pKa = 11.84VRR346 pKa = 11.84RR347 pKa = 11.84RR348 pKa = 11.84QRR350 pKa = 11.84RR351 pKa = 11.84RR352 pKa = 11.84RR353 pKa = 11.84AHH355 pKa = 5.74GARR358 pKa = 11.84RR359 pKa = 11.84ADD361 pKa = 3.81RR362 pKa = 11.84DD363 pKa = 3.47GARR366 pKa = 11.84ALPACGRR373 pKa = 11.84AGPARR378 pKa = 11.84GPRR381 pKa = 11.84PRR383 pKa = 11.84RR384 pKa = 11.84VGRR387 pKa = 11.84ASRR390 pKa = 11.84ARR392 pKa = 11.84RR393 pKa = 11.84PGRR396 pKa = 11.84GVPGFPADD404 pKa = 3.76PLGHH408 pKa = 6.55GGAGRR413 pKa = 11.84PAHH416 pKa = 5.71RR417 pKa = 11.84AVRR420 pKa = 11.84RR421 pKa = 11.84PAGRR425 pKa = 11.84GLRR428 pKa = 11.84SRR430 pKa = 11.84SQHH433 pKa = 4.28QGRR436 pKa = 11.84TRR438 pKa = 11.84RR439 pKa = 11.84RR440 pKa = 11.84RR441 pKa = 11.84GAGPGRR447 pKa = 11.84ARR449 pKa = 11.84ADD451 pKa = 3.33RR452 pKa = 11.84RR453 pKa = 11.84ARR455 pKa = 11.84SGPGRR460 pKa = 11.84PPAHH464 pKa = 7.47RR465 pKa = 11.84SRR467 pKa = 11.84PPGRR471 pKa = 11.84VRR473 pKa = 11.84RR474 pKa = 11.84RR475 pKa = 11.84RR476 pKa = 11.84PPGSRR481 pKa = 11.84PHH483 pKa = 6.16TPAPARR489 pKa = 11.84GGRR492 pKa = 11.84RR493 pKa = 11.84ACFRR497 pKa = 11.84RR498 pKa = 11.84VRR500 pKa = 11.84SGGPRR505 pKa = 11.84RR506 pKa = 11.84GRR508 pKa = 11.84GPRR511 pKa = 11.84RR512 pKa = 11.84PRR514 pKa = 11.84RR515 pKa = 11.84PGRR518 pKa = 11.84EE519 pKa = 3.91HH520 pKa = 6.9RR521 pKa = 11.84CRR523 pKa = 11.84PAGQSGRR530 pKa = 11.84PRR532 pKa = 11.84PGHH535 pKa = 6.7PGSAPARR542 pKa = 11.84RR543 pKa = 11.84GHH545 pKa = 5.59RR546 pKa = 11.84HH547 pKa = 4.96GRR549 pKa = 11.84PPGARR554 pKa = 11.84RR555 pKa = 11.84RR556 pKa = 11.84RR557 pKa = 11.84AVAARR562 pKa = 11.84PGRR565 pKa = 11.84PATRR569 pKa = 11.84RR570 pKa = 11.84ADD572 pKa = 3.44AAVPAGPHH580 pKa = 5.22RR581 pKa = 11.84EE582 pKa = 3.54RR583 pKa = 11.84RR584 pKa = 11.84GRR586 pKa = 11.84LRR588 pKa = 11.84RR589 pKa = 11.84ARR591 pKa = 11.84RR592 pKa = 11.84LGHH595 pKa = 6.94RR596 pKa = 11.84GRR598 pKa = 11.84AGPPDD603 pKa = 3.46RR604 pKa = 11.84VRR606 pKa = 11.84RR607 pKa = 11.84PRR609 pKa = 11.84SPGPAAASRR618 pKa = 11.84PPRR621 pKa = 11.84SDD623 pKa = 2.72RR624 pKa = 11.84AGRR627 pKa = 11.84RR628 pKa = 11.84TRR630 pKa = 11.84PARR633 pKa = 11.84PRR635 pKa = 11.84DD636 pKa = 3.59PRR638 pKa = 11.84QGRR641 pKa = 11.84HH642 pKa = 4.34RR643 pKa = 11.84TAARR647 pKa = 11.84PRR649 pKa = 11.84PDD651 pKa = 2.95RR652 pKa = 11.84RR653 pKa = 11.84RR654 pKa = 11.84VRR656 pKa = 11.84AARR659 pKa = 11.84AAARR663 pKa = 11.84RR664 pKa = 11.84AAGAALRR671 pKa = 11.84GPDD674 pKa = 3.02VPLRR678 pKa = 11.84HH679 pKa = 6.21RR680 pKa = 11.84PRR682 pKa = 11.84RR683 pKa = 11.84DD684 pKa = 2.93LHH686 pKa = 6.49RR687 pKa = 11.84DD688 pKa = 2.99RR689 pKa = 11.84TRR691 pKa = 11.84GVEE694 pKa = 3.91VGRR697 pKa = 11.84RR698 pKa = 11.84AALGRR703 pKa = 11.84PRR705 pKa = 11.84PSVCGRR711 pKa = 11.84DD712 pKa = 3.33PARR715 pKa = 11.84RR716 pKa = 11.84RR717 pKa = 11.84RR718 pKa = 11.84PGPRR722 pKa = 11.84AGAGPDD728 pKa = 3.08RR729 pKa = 11.84HH730 pKa = 5.63GTGPRR735 pKa = 11.84DD736 pKa = 3.32AGARR740 pKa = 11.84TADD743 pKa = 3.48RR744 pKa = 11.84RR745 pKa = 11.84PRR747 pKa = 11.84RR748 pKa = 11.84ARR750 pKa = 11.84AQRR753 pKa = 11.84RR754 pKa = 11.84RR755 pKa = 11.84AGPGRR760 pKa = 11.84SRR762 pKa = 11.84RR763 pKa = 11.84QRR765 pKa = 11.84RR766 pKa = 11.84PARR769 pKa = 11.84NPTGTADD776 pKa = 3.99RR777 pKa = 11.84GPAGARR783 pKa = 11.84RR784 pKa = 11.84PAAPVRR790 pKa = 11.84RR791 pKa = 11.84RR792 pKa = 11.84RR793 pKa = 11.84RR794 pKa = 11.84AARR797 pKa = 11.84LRR799 pKa = 11.84RR800 pKa = 11.84RR801 pKa = 11.84VHH803 pKa = 5.45LRR805 pKa = 11.84RR806 pKa = 11.84RR807 pKa = 11.84TAGRR811 pKa = 11.84PRR813 pKa = 11.84PRR815 pKa = 11.84AGGGLAVPAGPAPLRR830 pKa = 11.84GAAVAGAGGQHH841 pKa = 6.4PVDD844 pKa = 3.75GRR846 pKa = 11.84AGEE849 pKa = 4.18AVPVPGRR856 pKa = 11.84RR857 pKa = 11.84PGRR860 pKa = 11.84ALGKK864 pKa = 10.01RR865 pKa = 11.84PAPAADD871 pKa = 3.27VRR873 pKa = 11.84DD874 pKa = 4.02DD875 pKa = 3.4TRR877 pKa = 11.84VVHH880 pKa = 6.07RR881 pKa = 11.84HH882 pKa = 5.13RR883 pKa = 11.84LRR885 pKa = 11.84PRR887 pKa = 11.84PDD889 pKa = 2.89RR890 pKa = 11.84RR891 pKa = 11.84VRR893 pKa = 11.84APHH896 pKa = 5.62RR897 pKa = 11.84RR898 pKa = 11.84QRR900 pKa = 11.84RR901 pKa = 11.84THH903 pKa = 6.19RR904 pKa = 11.84PARR907 pKa = 11.84TAGRR911 pKa = 11.84AGGDD915 pKa = 3.05RR916 pKa = 11.84RR917 pKa = 11.84AGAGGPGTGRR927 pKa = 11.84SRR929 pKa = 11.84RR930 pKa = 11.84RR931 pKa = 11.84ADD933 pKa = 3.24RR934 pKa = 11.84RR935 pKa = 11.84RR936 pKa = 11.84RR937 pKa = 11.84GADD940 pKa = 2.76RR941 pKa = 11.84GRR943 pKa = 11.84ARR945 pKa = 11.84TRR947 pKa = 11.84RR948 pKa = 11.84RR949 pKa = 11.84TPHH952 pKa = 6.25AGDD955 pKa = 3.14RR956 pKa = 11.84AQRR959 pKa = 11.84VRR961 pKa = 11.84DD962 pKa = 3.73PGARR966 pKa = 11.84QQQGRR971 pKa = 11.84HH972 pKa = 4.82RR973 pKa = 11.84AAQTVAHH980 pKa = 6.41PQVQVLQAPGILVPAQAVAFRR1001 pKa = 11.84HH1002 pKa = 5.26AA1003 pKa = 4.09

Molecular weight:
111.65 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8394

0

8394

2700982

49

5850

321.8

34.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.532 ± 0.043

0.78 ± 0.008

5.807 ± 0.021

5.668 ± 0.03

2.956 ± 0.017

9.243 ± 0.026

2.17 ± 0.012

3.298 ± 0.02

2.26 ± 0.019

10.442 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.658 ± 0.012

1.928 ± 0.016

6.125 ± 0.024

2.879 ± 0.016

7.915 ± 0.034

5.359 ± 0.018

5.757 ± 0.021

8.705 ± 0.028

1.508 ± 0.011

2.008 ± 0.014

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski