Actinobacteria bacterium SCGC AG-212-D09

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; unclassified Actinobacteria

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 651 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177PYJ2|A0A177PYJ2_9ACTN Uncharacterized protein OS=Actinobacteria bacterium SCGC AG-212-D09 OX=1799652 GN=AYO39_01600 PE=4 SV=1
MM1 pKa = 6.85STEE4 pKa = 3.78ALFINVEE11 pKa = 3.84VDD13 pKa = 3.08EE14 pKa = 5.08SVANDD19 pKa = 3.49PALAAKK25 pKa = 8.55LTEE28 pKa = 4.13VCPVDD33 pKa = 5.16IFAQSDD39 pKa = 3.58DD40 pKa = 3.42GTLRR44 pKa = 11.84IVEE47 pKa = 4.43EE48 pKa = 4.12NLDD51 pKa = 3.79EE52 pKa = 5.13CVLCEE57 pKa = 4.18LCINAAPTGTVRR69 pKa = 11.84VIKK72 pKa = 10.2LYY74 pKa = 10.92SGEE77 pKa = 4.18TLPAA81 pKa = 4.79

Molecular weight: 8.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177PXF9|A0A177PXF9_9ACTN PNPLA domain-containing protein OS=Actinobacteria bacterium SCGC AG-212-D09 OX=1799652 GN=AYO39_03130 PE=4 SV=1
MM1 pKa = 7.64QMIVIGVDD9 pKa = 2.89THH11 pKa = 6.4KK12 pKa = 10.69RR13 pKa = 11.84SHH15 pKa = 6.0TLVALDD21 pKa = 4.34AGTGGARR28 pKa = 11.84GQLTIAASADD38 pKa = 3.38GTLEE42 pKa = 3.85ALRR45 pKa = 11.84FAAALDD51 pKa = 3.95SEE53 pKa = 4.87RR54 pKa = 11.84VWAVEE59 pKa = 3.94DD60 pKa = 3.73CRR62 pKa = 11.84HH63 pKa = 5.0VSGRR67 pKa = 11.84LEE69 pKa = 4.13RR70 pKa = 11.84EE71 pKa = 4.02LLSSGDD77 pKa = 3.21RR78 pKa = 11.84VVRR81 pKa = 11.84VAPGLTEE88 pKa = 3.72NSRR91 pKa = 11.84RR92 pKa = 11.84AARR95 pKa = 11.84QPGKK99 pKa = 10.24SDD101 pKa = 4.87PIDD104 pKa = 3.41ATAIARR110 pKa = 11.84AALRR114 pKa = 11.84EE115 pKa = 4.68GIDD118 pKa = 3.51TLPVAFLDD126 pKa = 4.29EE127 pKa = 4.17QAHH130 pKa = 6.45EE131 pKa = 4.04IRR133 pKa = 11.84VLNDD137 pKa = 2.7YY138 pKa = 10.45RR139 pKa = 11.84RR140 pKa = 11.84QLVAEE145 pKa = 4.52RR146 pKa = 11.84VRR148 pKa = 11.84LINRR152 pKa = 11.84LRR154 pKa = 11.84WHH156 pKa = 7.05LVQIAPEE163 pKa = 3.9IEE165 pKa = 4.16AQIRR169 pKa = 11.84PAGLIGPRR177 pKa = 11.84IRR179 pKa = 11.84AKK181 pKa = 8.54VTRR184 pKa = 11.84QLARR188 pKa = 11.84LPHH191 pKa = 5.84SPQLRR196 pKa = 11.84VAKK199 pKa = 10.45AILKK203 pKa = 8.81RR204 pKa = 11.84VCEE207 pKa = 4.14NYY209 pKa = 9.96RR210 pKa = 11.84EE211 pKa = 4.14EE212 pKa = 4.33NEE214 pKa = 4.26LLIEE218 pKa = 4.61LKK220 pKa = 9.96TLIDD224 pKa = 3.4AHH226 pKa = 6.79CPQLLNQRR234 pKa = 11.84GCGTVTAAIIIGHH247 pKa = 5.06TAGAKK252 pKa = 9.75RR253 pKa = 11.84FPTDD257 pKa = 2.44ACFARR262 pKa = 11.84HH263 pKa = 6.1TGTAPIPASSGNTQRR278 pKa = 11.84HH279 pKa = 4.86RR280 pKa = 11.84LHH282 pKa = 7.08RR283 pKa = 11.84GGDD286 pKa = 3.27RR287 pKa = 11.84QLNHH291 pKa = 7.19ALHH294 pKa = 6.98IIALSRR300 pKa = 11.84ARR302 pKa = 11.84TDD304 pKa = 3.39PATRR308 pKa = 11.84AYY310 pKa = 10.83LDD312 pKa = 3.43RR313 pKa = 11.84RR314 pKa = 11.84HH315 pKa = 6.14TEE317 pKa = 3.59GKK319 pKa = 7.83TKK321 pKa = 10.63KK322 pKa = 9.71EE323 pKa = 3.65ALRR326 pKa = 11.84CLKK329 pKa = 10.25RR330 pKa = 11.84HH331 pKa = 5.57LARR334 pKa = 11.84RR335 pKa = 11.84IWRR338 pKa = 11.84LLYY341 pKa = 8.23TTIEE345 pKa = 4.13AAPPRR350 pKa = 11.84TDD352 pKa = 3.19PSLEE356 pKa = 3.81KK357 pKa = 10.85SEE359 pKa = 4.55IPNFTT364 pKa = 3.53

Molecular weight: 40.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

651

0

651

184414

59

1282

283.3

30.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.857 ± 0.139

0.876 ± 0.032

5.533 ± 0.084

5.622 ± 0.129

3.088 ± 0.053

9.095 ± 0.13

2.152 ± 0.045

4.167 ± 0.064

2.108 ± 0.068

9.994 ± 0.134

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.662 ± 0.04

2.317 ± 0.066

5.882 ± 0.077

3.165 ± 0.058

8.122 ± 0.129

6.114 ± 0.092

5.818 ± 0.108

7.837 ± 0.081

1.436 ± 0.037

2.155 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski