Pseudomonas phage KPP23

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8VME6|W8VME6_9CAUD Uncharacterized protein OS=Pseudomonas phage KPP23 OX=1462581 GN=KPP23_075 PE=4 SV=1
MM1 pKa = 7.8NYY3 pKa = 9.97LQLIAVLLFFLFLFWIAWALFVKK26 pKa = 10.3SDD28 pKa = 3.91EE29 pKa = 4.1GSAYY33 pKa = 9.06YY34 pKa = 10.12TIDD37 pKa = 4.03GGNLCPFPDD46 pKa = 3.57MTDD49 pKa = 3.23EE50 pKa = 3.93QLYY53 pKa = 10.4DD54 pKa = 3.73YY55 pKa = 10.9EE56 pKa = 4.74SDD58 pKa = 3.38PFIGIKK64 pKa = 9.8PEE66 pKa = 3.63NRR68 pKa = 11.84TWPTLGDD75 pKa = 3.54YY76 pKa = 11.11LDD78 pKa = 3.61YY79 pKa = 11.31CRR81 pKa = 11.84ARR83 pKa = 11.84DD84 pKa = 3.7AKK86 pKa = 10.78KK87 pKa = 10.59EE88 pKa = 3.95NSNGG92 pKa = 3.42

Molecular weight: 10.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8VMA5|W8VMA5_9CAUD Uncharacterized protein OS=Pseudomonas phage KPP23 OX=1462581 GN=KPP23_030 PE=4 SV=1
MM1 pKa = 7.59TEE3 pKa = 3.85EE4 pKa = 3.58QLLAEE9 pKa = 4.31RR10 pKa = 11.84EE11 pKa = 4.11LRR13 pKa = 11.84VRR15 pKa = 11.84LLQLAEE21 pKa = 4.12NKK23 pKa = 10.35LLEE26 pKa = 4.12IDD28 pKa = 3.05KK29 pKa = 10.8ARR31 pKa = 11.84RR32 pKa = 11.84IRR34 pKa = 11.84FAFVLLVDD42 pKa = 5.42AIRR45 pKa = 11.84NSRR48 pKa = 11.84PDD50 pKa = 3.34EE51 pKa = 4.4LDD53 pKa = 2.86ALMRR57 pKa = 11.84DD58 pKa = 3.55SGVSRR63 pKa = 11.84WCKK66 pKa = 8.13TVVRR70 pKa = 11.84VIRR73 pKa = 11.84GSLQKK78 pKa = 10.54RR79 pKa = 11.84SVRR82 pKa = 11.84KK83 pKa = 9.84LEE85 pKa = 4.01YY86 pKa = 10.25NGRR89 pKa = 11.84TT90 pKa = 3.48

Molecular weight: 10.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

18816

44

907

198.1

21.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.56 ± 0.422

1.111 ± 0.135

5.814 ± 0.235

7.308 ± 0.31

4.22 ± 0.159

7.52 ± 0.24

1.424 ± 0.125

4.858 ± 0.177

5.325 ± 0.343

8.19 ± 0.182

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.11 ± 0.105

3.869 ± 0.218

4.751 ± 0.262

3.837 ± 0.277

7.153 ± 0.299

5.963 ± 0.185

5.57 ± 0.213

6.101 ± 0.226

1.286 ± 0.123

3.029 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski