uncultured phage MedDCM-OCT-S04-C26

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D6PH13|D6PH13_9CAUD Uncharacterized protein OS=uncultured phage MedDCM-OCT-S04-C26 OX=743544 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.42KK3 pKa = 10.25LLLFIIFLSPSSAFADD19 pKa = 3.28ITAKK23 pKa = 10.62YY24 pKa = 7.88VTAASFSIDD33 pKa = 3.26SPYY36 pKa = 10.92VITNAAPNTYY46 pKa = 10.0SISGNNITTSTGSGDD61 pKa = 3.94SIVTNGIGGLNLSSITNGLAGVTATNTTVTNAGSAFSLSEE101 pKa = 4.11SYY103 pKa = 10.48QAGDD107 pKa = 3.58ATQSAITPSSGIATLPVLGGQTTVISGGTLGSGSISSVSSGIHH150 pKa = 5.2SCSGAFGSGTSCTASTTVQIEE171 pKa = 3.53IDD173 pKa = 3.27

Molecular weight: 16.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D6PH40|D6PH40_9CAUD Uncharacterized protein OS=uncultured phage MedDCM-OCT-S04-C26 OX=743544 PE=4 SV=1
MM1 pKa = 7.47SIQRR5 pKa = 11.84GGHH8 pKa = 3.88TFEE11 pKa = 5.81RR12 pKa = 11.84VDD14 pKa = 3.91KK15 pKa = 10.58PIRR18 pKa = 11.84TPNHH22 pKa = 6.19SSGKK26 pKa = 8.47SHH28 pKa = 6.85AVVIKK33 pKa = 10.74KK34 pKa = 10.57GDD36 pKa = 3.5GYY38 pKa = 11.16RR39 pKa = 11.84LIRR42 pKa = 11.84FGMQGARR49 pKa = 11.84TKK51 pKa = 10.4PPRR54 pKa = 11.84KK55 pKa = 9.92GEE57 pKa = 4.01SDD59 pKa = 3.09ADD61 pKa = 3.47KK62 pKa = 11.14AKK64 pKa = 10.58RR65 pKa = 11.84KK66 pKa = 9.39SFKK69 pKa = 10.33ARR71 pKa = 11.84HH72 pKa = 5.86AKK74 pKa = 10.31NIAKK78 pKa = 10.14GKK80 pKa = 8.67TSAAYY85 pKa = 8.83WADD88 pKa = 3.27KK89 pKa = 10.73VKK91 pKa = 9.86WW92 pKa = 3.5

Molecular weight: 10.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

9674

54

542

164.0

17.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.6 ± 0.529

0.868 ± 0.101

5.541 ± 0.221

5.479 ± 0.402

4.114 ± 0.195

6.926 ± 0.478

1.395 ± 0.163

6.347 ± 0.261

6.626 ± 0.626

7.391 ± 0.288

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.067 ± 0.165

5.634 ± 0.288

3.897 ± 0.276

4.083 ± 0.258

4.135 ± 0.319

7.99 ± 0.52

8.735 ± 0.633

6.285 ± 0.224

0.879 ± 0.137

3.008 ± 0.212
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski