Roseobacter phage CRP-3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A646QVZ6|A0A646QVZ6_9CAUD Uncharacterized protein OS=Roseobacter phage CRP-3 OX=2559282 GN=CRP3_gp46 PE=4 SV=1
MM1 pKa = 7.64AGYY4 pKa = 7.86TRR6 pKa = 11.84QSTSQIINGANITAPPLNAEE26 pKa = 4.47FNQLASSFGVTGHH39 pKa = 5.4THH41 pKa = 7.57DD42 pKa = 4.42GTSGNAPKK50 pKa = 9.83IDD52 pKa = 3.77LATSVTGYY60 pKa = 10.97LPATNGGTGGKK71 pKa = 9.71NNLAATTNPGNGDD84 pKa = 3.92DD85 pKa = 5.43ADD87 pKa = 4.35DD88 pKa = 4.92GYY90 pKa = 11.99SRR92 pKa = 11.84GSYY95 pKa = 8.01WYY97 pKa = 10.17NYY99 pKa = 9.25TGDD102 pKa = 3.11RR103 pKa = 11.84WYY105 pKa = 10.09ICINNTVGSAVWRR118 pKa = 11.84EE119 pKa = 3.77MLMVEE124 pKa = 4.35TGSVIEE130 pKa = 4.37PGITGAVDD138 pKa = 3.96LGSTTFKK145 pKa = 11.01FKK147 pKa = 9.73DD148 pKa = 3.34THH150 pKa = 7.28LSGSMNSVSAAISGNITVGGTQTNTGAATFNGSATFNNITNFNGASNTIANAAITSGTINGTTIGGATAAAGTFTALTANNSANLTNATISGGTINNSVIGGTTPLNITGLTVAASTGFSGDD272 pKa = 2.95LTGNVLGNVTGNLTGTVNATGGVIGDD298 pKa = 3.4VTGNVTASSGSSTFNNVVINGSLDD322 pKa = 3.25MDD324 pKa = 3.85ATTASTITGLSTPVNPTDD342 pKa = 3.94ASTKK346 pKa = 10.44AYY348 pKa = 10.69VDD350 pKa = 3.42QQVALVLSSAPAALDD365 pKa = 3.51TLNEE369 pKa = 3.77LAAAINDD376 pKa = 3.85DD377 pKa = 4.31ANFATTVNNSIATKK391 pKa = 10.36LSLSGGTMSGNIDD404 pKa = 3.74LNSTNKK410 pKa = 7.44ITNMPTPSANSDD422 pKa = 3.44GSNKK426 pKa = 10.13GYY428 pKa = 11.0VDD430 pKa = 3.49TQRR433 pKa = 11.84DD434 pKa = 3.48TRR436 pKa = 11.84LATTGGTMSGAINMNSQAITSLANPANSGDD466 pKa = 3.83AANKK470 pKa = 10.02FYY472 pKa = 11.14VDD474 pKa = 4.79SILGSATSAAASASTASALAAQASGSAANAQASEE508 pKa = 4.73DD509 pKa = 4.12EE510 pKa = 4.27AQEE513 pKa = 4.03WATKK517 pKa = 6.57TTGTITGEE525 pKa = 4.03SEE527 pKa = 4.16YY528 pKa = 10.83SAKK531 pKa = 10.18EE532 pKa = 3.8YY533 pKa = 10.79AIGTVIRR540 pKa = 11.84GNIGSAKK547 pKa = 10.2DD548 pKa = 2.96WSTYY552 pKa = 9.59TGGTVDD558 pKa = 3.57GTNYY562 pKa = 9.26SAKK565 pKa = 10.26YY566 pKa = 8.05WATNANIGTIASNIDD581 pKa = 3.45DD582 pKa = 4.62LVNVANDD589 pKa = 3.91LSSGNFVAGQIYY601 pKa = 10.37DD602 pKa = 3.72FGSITDD608 pKa = 3.9AASGTSGAPDD618 pKa = 3.55GFIVTVANNLADD630 pKa = 3.74VQTVSNVITNVNTVAGISANVTTVAGVSSAVSTLAAISSDD670 pKa = 3.38VSAVAPIAANVTTVAGVSSDD690 pKa = 3.42VTAVANNNANINTVVGQITPNNNISTLASIAGNISTVASVSVDD733 pKa = 3.21VTTVAGISSNVTAVANNNANVTSVANIDD761 pKa = 3.62SDD763 pKa = 4.0VTAVAGIASDD773 pKa = 3.68VTTAATNVTDD783 pKa = 3.33ITNFADD789 pKa = 4.38VYY791 pKa = 10.5QGPKK795 pKa = 8.55ATAPTQRR802 pKa = 11.84NNNSSLVAGDD812 pKa = 4.72LYY814 pKa = 11.31FDD816 pKa = 3.91TVSGFMRR823 pKa = 11.84YY824 pKa = 9.11YY825 pKa = 10.78SGSSWSNITAPTGDD839 pKa = 3.8MGNQNSTSVAITGGSITGITDD860 pKa = 3.8LEE862 pKa = 4.37IADD865 pKa = 4.53GGTGASSAPAARR877 pKa = 11.84TNLGLDD883 pKa = 3.51TMATQAANNVAITGGTISVDD903 pKa = 3.79FDD905 pKa = 3.9FGSII909 pKa = 3.53

Molecular weight: 90.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A646QX19|A0A646QX19_9CAUD Uncharacterized protein OS=Roseobacter phage CRP-3 OX=2559282 GN=CRP3_gp03 PE=4 SV=1
MM1 pKa = 7.48YY2 pKa = 10.45ILGANFLLAGQLKK15 pKa = 9.48IRR17 pKa = 11.84AALRR21 pKa = 11.84GGFFLTAPRR30 pKa = 11.84AIFRR34 pKa = 11.84RR35 pKa = 11.84FEE37 pKa = 3.86WGVIGPGILTQKK49 pKa = 10.56NPFPVFSGDD58 pKa = 3.28LVFFRR63 pKa = 11.84VYY65 pKa = 10.44FMAFCTVFFTPLYY78 pKa = 9.23IVRR81 pKa = 11.84SIHH84 pKa = 6.13NLGKK88 pKa = 10.43VFPHH92 pKa = 7.43LIKK95 pKa = 10.92CLL97 pKa = 3.55

Molecular weight: 11.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

16287

43

1292

271.4

29.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.603 ± 0.545

0.786 ± 0.128

6.907 ± 0.304

6.766 ± 0.445

3.764 ± 0.181

8.479 ± 0.589

1.351 ± 0.208

5.25 ± 0.172

5.391 ± 0.398

7.472 ± 0.303

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.935 ± 0.226

5.047 ± 0.336

3.917 ± 0.302

4.071 ± 0.262

5.004 ± 0.321

6.576 ± 0.319

6.564 ± 0.451

6.011 ± 0.257

1.068 ± 0.132

3.039 ± 0.2
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski