Staphylococcus phage PT1028

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified dsDNA phages

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4ZE87|Q4ZE87_9VIRU ORF010 OS=Staphylococcus phage PT1028 OX=320831 PE=4 SV=1
MM1 pKa = 8.27DD2 pKa = 3.86KK3 pKa = 10.53QQIKK7 pKa = 10.78GFVCDD12 pKa = 3.34YY13 pKa = 10.94HH14 pKa = 6.77EE15 pKa = 4.34RR16 pKa = 11.84TRR18 pKa = 11.84SDD20 pKa = 3.32VLIDD24 pKa = 4.43DD25 pKa = 6.21DD26 pKa = 4.79INTDD30 pKa = 3.47EE31 pKa = 4.82FFSIGDD37 pKa = 3.67EE38 pKa = 4.7NSNEE42 pKa = 3.41WMADD46 pKa = 3.53DD47 pKa = 5.85NIDD50 pKa = 3.4DD51 pKa = 5.39HH52 pKa = 7.99IVKK55 pKa = 9.81NHH57 pKa = 6.7LEE59 pKa = 4.22MIVDD63 pKa = 3.54QVANDD68 pKa = 3.42KK69 pKa = 10.67EE70 pKa = 4.63FYY72 pKa = 10.6IFDD75 pKa = 3.75SLIQGRR81 pKa = 11.84SFKK84 pKa = 10.75DD85 pKa = 2.86ISNVLEE91 pKa = 4.44CSEE94 pKa = 3.9QSVRR98 pKa = 11.84LWYY101 pKa = 7.65EE102 pKa = 3.57TLLDD106 pKa = 4.5KK107 pKa = 10.73IVEE110 pKa = 4.48VIEE113 pKa = 4.08

Molecular weight: 13.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4ZE87|Q4ZE87_9VIRU ORF010 OS=Staphylococcus phage PT1028 OX=320831 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.42LLKK5 pKa = 9.67TKK7 pKa = 10.46NCLYY11 pKa = 10.4YY12 pKa = 10.86RR13 pKa = 11.84NGDD16 pKa = 3.6NKK18 pKa = 10.58LSEE21 pKa = 4.42YY22 pKa = 10.79QLLTQFNPAFINKK35 pKa = 8.6KK36 pKa = 9.68IKK38 pKa = 9.14MCEE41 pKa = 3.79FQIEE45 pKa = 4.59SMYY48 pKa = 10.71HH49 pKa = 6.07LSASTTTCDD58 pKa = 3.38EE59 pKa = 3.56IMGIVSVSYY68 pKa = 9.84PIEE71 pKa = 4.11KK72 pKa = 10.29LVIKK76 pKa = 10.38IIEE79 pKa = 4.29TKK81 pKa = 10.8ARR83 pKa = 11.84LQNYY87 pKa = 8.67KK88 pKa = 10.27NRR90 pKa = 11.84SISNMVLLKK99 pKa = 10.01TVLNHH104 pKa = 4.98YY105 pKa = 7.27TEE107 pKa = 5.21KK108 pKa = 10.55EE109 pKa = 3.81QKK111 pKa = 10.14QVVKK115 pKa = 10.31YY116 pKa = 8.17MRR118 pKa = 11.84SNGRR122 pKa = 11.84YY123 pKa = 8.61KK124 pKa = 10.23PYY126 pKa = 10.97NVIEE130 pKa = 4.13RR131 pKa = 11.84LQVDD135 pKa = 5.23LYY137 pKa = 10.41QASIKK142 pKa = 9.94QRR144 pKa = 11.84SEE146 pKa = 3.59RR147 pKa = 11.84QKK149 pKa = 10.33QRR151 pKa = 11.84NIAIEE156 pKa = 3.78NSKK159 pKa = 9.76IARR162 pKa = 11.84VNAYY166 pKa = 8.94HH167 pKa = 6.26QSSHH171 pKa = 5.06VKK173 pKa = 9.97VVV175 pKa = 2.88

Molecular weight: 20.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22

0

22

4111

60

569

186.9

21.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.965 ± 0.382

0.803 ± 0.151

5.887 ± 0.378

7.808 ± 0.564

3.77 ± 0.377

4.816 ± 0.584

2.311 ± 0.326

7.735 ± 0.271

9.852 ± 0.66

8.562 ± 0.34

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.141 ± 0.178

7.297 ± 0.357

2.287 ± 0.27

3.843 ± 0.322

3.77 ± 0.281

7.37 ± 0.56

5.522 ± 0.521

6.106 ± 0.435

0.924 ± 0.122

5.23 ± 0.382
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski