Blautia sp. An249

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Blautia; unclassified Blautia

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3735 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4F6F3|A0A1Y4F6F3_9FIRM TM1586_NiRdase domain-containing protein OS=Blautia sp. An249 OX=1965603 GN=B5F53_09470 PE=4 SV=1
MM1 pKa = 7.42SRR3 pKa = 11.84KK4 pKa = 9.38HH5 pKa = 6.18KK6 pKa = 10.74NLVILATVLSAACIFSACGKK26 pKa = 10.06DD27 pKa = 4.04KK28 pKa = 10.91EE29 pKa = 4.48DD30 pKa = 3.36QVVVAEE36 pKa = 4.94APTPTPTVAPTITPVPSVAATAQKK60 pKa = 8.9VTYY63 pKa = 8.48TSKK66 pKa = 10.81DD67 pKa = 3.35SSVSIVLPDD76 pKa = 3.66NTWKK80 pKa = 8.81NTSDD84 pKa = 3.3NGKK87 pKa = 10.49SIIFNSDD94 pKa = 2.66TQGNISITYY103 pKa = 10.1ANTDD107 pKa = 2.92ADD109 pKa = 4.17LAKK112 pKa = 10.81VQIPSSKK119 pKa = 9.94EE120 pKa = 3.42LAGSITAGTSLTEE133 pKa = 4.06GTDD136 pKa = 4.15FDD138 pKa = 4.14ILDD141 pKa = 3.74YY142 pKa = 10.51TATEE146 pKa = 3.87MDD148 pKa = 3.43GVDD151 pKa = 3.27VYY153 pKa = 11.18RR154 pKa = 11.84YY155 pKa = 6.55TVKK158 pKa = 10.76AADD161 pKa = 3.47ASKK164 pKa = 9.96MNGYY168 pKa = 10.79AYY170 pKa = 9.76IEE172 pKa = 4.2NYY174 pKa = 10.34YY175 pKa = 9.21LTDD178 pKa = 3.67GSEE181 pKa = 4.69LYY183 pKa = 10.52QISGSLKK190 pKa = 10.28LQDD193 pKa = 3.69PTSLNTMKK201 pKa = 10.62EE202 pKa = 3.96AVTSFRR208 pKa = 11.84ILKK211 pKa = 10.1ADD213 pKa = 3.79SPLKK217 pKa = 10.61AAADD221 pKa = 4.47TIASQNSSSATPSVTPSVTPAASGDD246 pKa = 3.79SDD248 pKa = 4.52NSDD251 pKa = 3.52SSDD254 pKa = 4.11DD255 pKa = 3.78SDD257 pKa = 4.25TNTSSTSDD265 pKa = 3.3GSSSDD270 pKa = 2.98SSYY273 pKa = 11.77DD274 pKa = 3.63DD275 pKa = 4.03SSSSGDD281 pKa = 3.47DD282 pKa = 3.3SGSGNSGQSSEE293 pKa = 4.71DD294 pKa = 2.71GWYY297 pKa = 10.42DD298 pKa = 3.18GDD300 pKa = 4.06TFISYY305 pKa = 11.09DD306 pKa = 3.82DD307 pKa = 5.31GYY309 pKa = 11.46FDD311 pKa = 6.73DD312 pKa = 5.4DD313 pKa = 6.31GYY315 pKa = 9.33WHH317 pKa = 7.73WYY319 pKa = 9.76GDD321 pKa = 3.81DD322 pKa = 3.96SGDD325 pKa = 5.07DD326 pKa = 3.74GSGDD330 pKa = 4.06DD331 pKa = 4.76GSDD334 pKa = 4.28DD335 pKa = 3.87GSSDD339 pKa = 4.85DD340 pKa = 4.73GSGDD344 pKa = 3.95DD345 pKa = 5.63GSGSDD350 pKa = 4.92DD351 pKa = 4.49GGWYY355 pKa = 10.01DD356 pKa = 3.78NNGNYY361 pKa = 9.72FSYY364 pKa = 11.35NDD366 pKa = 4.75GYY368 pKa = 11.39FDD370 pKa = 6.87DD371 pKa = 5.77DD372 pKa = 6.31GVWQWYY378 pKa = 8.98

Molecular weight: 39.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4F598|A0A1Y4F598_9FIRM Inosine monophosphate cyclohydrolase OS=Blautia sp. An249 OX=1965603 GN=B5F53_09430 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.89TKK25 pKa = 10.34GGRR28 pKa = 11.84KK29 pKa = 8.67VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.68GRR39 pKa = 11.84KK40 pKa = 8.28QLSAA44 pKa = 3.9

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3735

0

3735

1167479

37

3650

312.6

35.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.321 ± 0.044

1.516 ± 0.018

5.229 ± 0.037

8.243 ± 0.045

4.037 ± 0.026

7.262 ± 0.038

1.673 ± 0.016

7.037 ± 0.038

6.852 ± 0.039

9.16 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.99 ± 0.022

3.987 ± 0.025

3.409 ± 0.022

3.654 ± 0.021

4.639 ± 0.03

5.824 ± 0.038

5.426 ± 0.039

6.638 ± 0.036

0.971 ± 0.014

4.132 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski