Mycobacterium phage Marshawn

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8D9U3|A0A5P8D9U3_9CAUD Uncharacterized protein OS=Mycobacterium phage Marshawn OX=2652423 GN=74 PE=4 SV=1
MM1 pKa = 7.71SYY3 pKa = 10.2HH4 pKa = 6.32YY5 pKa = 9.76CTGDD9 pKa = 3.63DD10 pKa = 4.18CGHH13 pKa = 6.7CARR16 pKa = 11.84RR17 pKa = 11.84IDD19 pKa = 3.51QAEE22 pKa = 4.24YY23 pKa = 10.55EE24 pKa = 4.14RR25 pKa = 11.84DD26 pKa = 3.53VYY28 pKa = 11.52GDD30 pKa = 4.16DD31 pKa = 5.82DD32 pKa = 4.44YY33 pKa = 11.97PDD35 pKa = 4.53YY36 pKa = 11.71YY37 pKa = 11.27DD38 pKa = 3.5GTT40 pKa = 4.14

Molecular weight: 4.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8D9V4|A0A5P8D9V4_9CAUD Uncharacterized protein OS=Mycobacterium phage Marshawn OX=2652423 GN=84 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.25RR3 pKa = 11.84TRR5 pKa = 11.84TYY7 pKa = 9.96RR8 pKa = 11.84APAVAVDD15 pKa = 3.81RR16 pKa = 11.84PEE18 pKa = 4.05VVVNGVTLEE27 pKa = 4.23PGQEE31 pKa = 3.66VSIRR35 pKa = 11.84GEE37 pKa = 3.58RR38 pKa = 11.84GRR40 pKa = 11.84YY41 pKa = 9.05RR42 pKa = 11.84FVKK45 pKa = 9.95SARR48 pKa = 11.84TSSGRR53 pKa = 11.84VTCDD57 pKa = 4.2FIGPDD62 pKa = 4.12DD63 pKa = 3.76QTKK66 pKa = 9.74CWRR69 pKa = 11.84SFYY72 pKa = 9.91PEE74 pKa = 4.32RR75 pKa = 11.84IKK77 pKa = 10.04TVHH80 pKa = 5.69RR81 pKa = 11.84VSRR84 pKa = 11.84TRR86 pKa = 11.84ANAAA90 pKa = 3.24

Molecular weight: 10.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

18777

36

1334

197.7

21.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.437 ± 0.488

1.097 ± 0.138

6.641 ± 0.243

5.635 ± 0.337

2.732 ± 0.159

8.942 ± 0.491

2.226 ± 0.162

3.685 ± 0.172

3.046 ± 0.218

8.404 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.306 ± 0.092

2.546 ± 0.14

5.651 ± 0.248

3.137 ± 0.163

7.206 ± 0.391

4.878 ± 0.184

6.002 ± 0.209

8.026 ± 0.246

2.077 ± 0.122

2.327 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski