Eubacterium sp. CAG:581

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; environmental samples

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1746 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7NMR9|R7NMR9_9FIRM Glutamate--tRNA ligase OS=Eubacterium sp. CAG:581 OX=1262890 GN=gltX PE=3 SV=1
MM1 pKa = 7.64KK2 pKa = 10.43KK3 pKa = 10.36NISIKK8 pKa = 10.85GLVLLLALCLVGSFAMYY25 pKa = 10.7GCGSDD30 pKa = 4.76DD31 pKa = 3.81KK32 pKa = 11.27SSSKK36 pKa = 10.95ADD38 pKa = 3.29SSASDD43 pKa = 3.52TADD46 pKa = 3.59KK47 pKa = 11.36SSDD50 pKa = 3.33DD51 pKa = 4.2SKK53 pKa = 11.93NSDD56 pKa = 3.88EE57 pKa = 5.42NSDD60 pKa = 5.13DD61 pKa = 3.67DD62 pKa = 4.31TQVADD67 pKa = 4.08ITGQWVLDD75 pKa = 3.98QIVDD79 pKa = 3.62ADD81 pKa = 4.24GNSQTLEE88 pKa = 4.34EE89 pKa = 4.3YY90 pKa = 10.58CAAQGVDD97 pKa = 3.27SSGVDD102 pKa = 3.21VTYY105 pKa = 10.4TFSEE109 pKa = 4.58DD110 pKa = 3.21GTLVGAVGGVGVEE123 pKa = 4.06GTYY126 pKa = 10.78EE127 pKa = 4.04FDD129 pKa = 4.02GSTVSCTISDD139 pKa = 3.64TTSNFDD145 pKa = 3.55YY146 pKa = 11.14NADD149 pKa = 4.32DD150 pKa = 3.94DD151 pKa = 4.75TISYY155 pKa = 10.74SDD157 pKa = 4.0EE158 pKa = 4.05NTGLSSVLVRR168 pKa = 11.84KK169 pKa = 9.51

Molecular weight: 17.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7NQ27|R7NQ27_9FIRM 3-oxoacyl-[acyl-carrier-protein] synthase 2 OS=Eubacterium sp. CAG:581 OX=1262890 GN=BN720_00035 PE=3 SV=1
MM1 pKa = 8.04ADD3 pKa = 3.43RR4 pKa = 11.84PNRR7 pKa = 11.84GRR9 pKa = 11.84KK10 pKa = 8.04SRR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 9.03VCGFCVDD21 pKa = 3.39KK22 pKa = 11.41VEE24 pKa = 4.93KK25 pKa = 9.78IDD27 pKa = 3.73YY28 pKa = 10.45KK29 pKa = 11.11DD30 pKa = 2.93IARR33 pKa = 11.84LRR35 pKa = 11.84RR36 pKa = 11.84YY37 pKa = 8.36MSEE40 pKa = 3.79RR41 pKa = 11.84AKK43 pKa = 10.39ILPRR47 pKa = 11.84RR48 pKa = 11.84VTGTCAAHH56 pKa = 5.7QRR58 pKa = 11.84EE59 pKa = 4.66LTVAIKK65 pKa = 10.33RR66 pKa = 11.84ARR68 pKa = 11.84QIALLPYY75 pKa = 10.15TSDD78 pKa = 3.02

Molecular weight: 9.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1746

0

1746

525267

30

1479

300.8

33.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.128 ± 0.055

1.597 ± 0.025

6.076 ± 0.052

6.502 ± 0.062

4.31 ± 0.05

6.624 ± 0.052

1.479 ± 0.026

8.057 ± 0.059

8.697 ± 0.057

8.187 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.75 ± 0.031

5.981 ± 0.047

3.005 ± 0.031

2.423 ± 0.028

3.28 ± 0.044

6.533 ± 0.055

6.068 ± 0.062

7.294 ± 0.049

0.677 ± 0.018

4.327 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski