Blackbird associated gemykibivirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus blabi1

Average proteome isoelectric point is 7.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T1YSF1|T1YSF1_9VIRU Replication-associated protein OS=Blackbird associated gemykibivirus 1 OX=1391038 PE=3 SV=1
MM1 pKa = 7.83SSFRR5 pKa = 11.84FQARR9 pKa = 11.84YY10 pKa = 9.94ALLTYY15 pKa = 8.97AQCGDD20 pKa = 4.18LDD22 pKa = 3.95PFAVSDD28 pKa = 3.87HH29 pKa = 6.55LSGLRR34 pKa = 11.84AEE36 pKa = 4.86CIIGRR41 pKa = 11.84EE42 pKa = 3.95THH44 pKa = 6.43ADD46 pKa = 3.41EE47 pKa = 6.26GIHH50 pKa = 6.09LHH52 pKa = 6.33AFVDD56 pKa = 4.63FGVKK60 pKa = 10.14FSTRR64 pKa = 11.84NVRR67 pKa = 11.84VFDD70 pKa = 4.09VQGHH74 pKa = 5.85HH75 pKa = 7.09PNVEE79 pKa = 3.99PSRR82 pKa = 11.84GRR84 pKa = 11.84PAGGYY89 pKa = 10.24DD90 pKa = 3.5YY91 pKa = 10.78AIKK94 pKa = 10.94DD95 pKa = 3.59GDD97 pKa = 4.09VVAGGLDD104 pKa = 3.27RR105 pKa = 11.84PADD108 pKa = 4.1DD109 pKa = 4.66GGVGVSKK116 pKa = 7.8TTQKK120 pKa = 9.89WATIVGAQTRR130 pKa = 11.84DD131 pKa = 3.63EE132 pKa = 4.66FWALLAEE139 pKa = 5.02LDD141 pKa = 3.64PGAMVRR147 pKa = 11.84SFTQCRR153 pKa = 11.84AYY155 pKa = 10.32ADD157 pKa = 3.32HH158 pKa = 7.51RR159 pKa = 11.84FRR161 pKa = 11.84EE162 pKa = 4.35SPEE165 pKa = 4.09RR166 pKa = 11.84YY167 pKa = 7.51VTPEE171 pKa = 4.04SIIIDD176 pKa = 3.72TGHH179 pKa = 6.44VDD181 pKa = 5.5GLNEE185 pKa = 3.79WALSNIVGHH194 pKa = 6.24RR195 pKa = 11.84RR196 pKa = 11.84GGIGKK201 pKa = 9.29SLVLYY206 pKa = 10.73GPTRR210 pKa = 11.84TGKK213 pKa = 7.51TVWARR218 pKa = 11.84SLGDD222 pKa = 3.2HH223 pKa = 7.09AYY225 pKa = 10.49FGGLFSMDD233 pKa = 3.83EE234 pKa = 4.95DD235 pKa = 3.74ISSATYY241 pKa = 10.75AVFDD245 pKa = 4.61DD246 pKa = 4.28FGGLKK251 pKa = 10.18FLPSYY256 pKa = 9.33KK257 pKa = 9.98FWMGHH262 pKa = 4.03QKK264 pKa = 9.93QFYY267 pKa = 8.81VTDD270 pKa = 3.67KK271 pKa = 11.21YY272 pKa = 10.96KK273 pKa = 10.95GKK275 pKa = 10.67KK276 pKa = 8.39LVEE279 pKa = 4.02WGRR282 pKa = 11.84PSIWINNEE290 pKa = 3.65DD291 pKa = 3.53PRR293 pKa = 11.84EE294 pKa = 4.01EE295 pKa = 4.08HH296 pKa = 6.85GVRR299 pKa = 11.84CDD301 pKa = 3.5EE302 pKa = 5.25IEE304 pKa = 4.02WLEE307 pKa = 3.93QNCIFIRR314 pKa = 11.84LDD316 pKa = 3.28STIVRR321 pKa = 11.84ARR323 pKa = 11.84IDD325 pKa = 3.11

Molecular weight: 36.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T1YSF1|T1YSF1_9VIRU Replication-associated protein OS=Blackbird associated gemykibivirus 1 OX=1391038 PE=3 SV=1
MM1 pKa = 7.69AFRR4 pKa = 11.84RR5 pKa = 11.84FRR7 pKa = 11.84NTQRR11 pKa = 11.84RR12 pKa = 11.84PIQGRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84VVRR22 pKa = 11.84TRR24 pKa = 11.84HH25 pKa = 4.84RR26 pKa = 11.84RR27 pKa = 11.84VMRR30 pKa = 11.84TRR32 pKa = 11.84RR33 pKa = 11.84SRR35 pKa = 11.84VPKK38 pKa = 10.16RR39 pKa = 11.84AILNLVSRR47 pKa = 11.84KK48 pKa = 9.72KK49 pKa = 9.98RR50 pKa = 11.84DD51 pKa = 3.16AMAGLSQTAGTQNNGPLVMTANTTNSGFGVFVVPWVPTARR91 pKa = 11.84DD92 pKa = 3.6FFVNPSGNHH101 pKa = 5.86SDD103 pKa = 4.5INDD106 pKa = 3.24QSTRR110 pKa = 11.84TAQRR114 pKa = 11.84CFIRR118 pKa = 11.84GVQEE122 pKa = 4.47RR123 pKa = 11.84IRR125 pKa = 11.84MYY127 pKa = 10.62TSTSLPWEE135 pKa = 3.57WRR137 pKa = 11.84RR138 pKa = 11.84IVFAFKK144 pKa = 9.43GTAIMLPIGSAFTPYY159 pKa = 10.55YY160 pKa = 10.63ADD162 pKa = 3.94SSSGWRR168 pKa = 11.84RR169 pKa = 11.84YY170 pKa = 9.71ALNLTATTSTSDD182 pKa = 4.02QIASANNLLGFMFRR196 pKa = 11.84GRR198 pKa = 11.84IGQDD202 pKa = 2.96YY203 pKa = 10.32FDD205 pKa = 5.6PLTAITDD212 pKa = 3.8TNIVKK217 pKa = 10.18IMSDD221 pKa = 3.13RR222 pKa = 11.84TRR224 pKa = 11.84RR225 pKa = 11.84IASSNNSGTQKK236 pKa = 10.33VYY238 pKa = 11.14KK239 pKa = 10.02LWHH242 pKa = 6.43PVNKK246 pKa = 9.16TLVYY250 pKa = 10.48SDD252 pKa = 5.0SEE254 pKa = 4.91DD255 pKa = 3.78GDD257 pKa = 3.93EE258 pKa = 4.33EE259 pKa = 4.48DD260 pKa = 5.62AGFLSTPGRR269 pKa = 11.84PGCGDD274 pKa = 3.5IYY276 pKa = 11.26VVDD279 pKa = 3.87IFKK282 pKa = 9.8PHH284 pKa = 7.11PSASTDD290 pKa = 2.89ILSFGPEE297 pKa = 3.59ASLFWHH303 pKa = 6.46EE304 pKa = 4.15RR305 pKa = 3.36

Molecular weight: 34.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

630

305

325

315.0

35.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.143 ± 0.173

1.111 ± 0.305

6.984 ± 0.946

3.968 ± 1.122

5.556 ± 0.012

8.73 ± 1.237

2.698 ± 0.71

5.556 ± 0.012

3.492 ± 0.363

5.873 ± 0.421

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.905 ± 0.482

3.651 ± 1.07

4.444 ± 0.537

3.016 ± 0.176

9.683 ± 1.422

7.302 ± 1.04

6.825 ± 1.359

6.667 ± 0.293

2.222 ± 0.171

3.175 ± 0.37
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski