Budgerigar fledgling disease virus - 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Gammapolyomavirus; Aves polyomavirus 1

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9IBL3|Q9IBL3_BFPYV Large T antigen OS=Budgerigar fledgling disease virus - 1 OX=133789 PE=4 SV=1
MM1 pKa = 7.13STPARR6 pKa = 11.84DD7 pKa = 3.44PNTAGTAALSPFSTPNHH24 pKa = 5.79EE25 pKa = 4.42LRR27 pKa = 11.84APGPGEE33 pKa = 3.59AHH35 pKa = 6.97SPFTPTAAPGSQPAGSLSDD54 pKa = 4.5PEE56 pKa = 5.47DD57 pKa = 4.64GPDD60 pKa = 3.35PTFNFYY66 pKa = 10.23IQPNSPTPTATTPEE80 pKa = 4.24ASPAAPTTEE89 pKa = 4.29STEE92 pKa = 4.19TTGPSVATNATEE104 pKa = 4.09PSEE107 pKa = 4.25SRR109 pKa = 11.84PARR112 pKa = 3.7

Molecular weight: 11.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E4MVD7|E4MVD7_BFPYV Agnoprotein 2b OS=Budgerigar fledgling disease virus - 1 OX=133789 PE=4 SV=1
MM1 pKa = 7.76PNGTPTLNRR10 pKa = 11.84PLARR14 pKa = 11.84LALRR18 pKa = 11.84ITSCAHH24 pKa = 5.48QGLEE28 pKa = 3.75RR29 pKa = 11.84LIRR32 pKa = 11.84LLHH35 pKa = 6.34LLLHH39 pKa = 6.85LGRR42 pKa = 11.84SLLDD46 pKa = 3.37LFQTLRR52 pKa = 11.84TALTQPSTSIFSLTRR67 pKa = 11.84LPLLLPPLRR76 pKa = 11.84LLLLLLLPSPPRR88 pKa = 11.84QPALASRR95 pKa = 11.84LTQPNRR101 pKa = 11.84PNRR104 pKa = 11.84GRR106 pKa = 11.84LAKK109 pKa = 10.46

Molecular weight: 12.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

2139

79

599

237.7

26.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.322 ± 1.055

1.356 ± 0.507

4.956 ± 0.673

6.732 ± 0.784

3.273 ± 0.466

6.358 ± 1.045

2.057 ± 0.419

4.535 ± 0.68

4.208 ± 0.85

10.519 ± 1.59

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.104 ± 0.286

3.693 ± 0.269

7.62 ± 1.266

4.628 ± 0.517

6.826 ± 0.695

5.704 ± 0.832

8.275 ± 0.867

5.002 ± 0.745

0.888 ± 0.329

2.945 ± 0.398
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski