Cynara cardunculus var. scolymus (Globe artichoke) (Cynara scolymus)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; campanulids; Asterales; Asteraceae;

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26434 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A103Y6C1|A0A103Y6C1_CYNCS Chlorophyll a-b binding protein chloroplastic OS=Cynara cardunculus var. scolymus OX=59895 GN=Ccrd_018365 PE=3 SV=1
MM1 pKa = 7.84ADD3 pKa = 3.33MSNIQALSLQPNTPQHH19 pKa = 5.26QVLEE23 pKa = 4.33EE24 pKa = 4.1EE25 pKa = 5.0EE26 pKa = 4.1EE27 pKa = 4.55DD28 pKa = 3.64EE29 pKa = 4.99TISSSQQTLDD39 pKa = 3.79NSITNCSSLVEE50 pKa = 4.22EE51 pKa = 5.06EE52 pKa = 4.57EE53 pKa = 5.24EE54 pKa = 4.87EE55 pKa = 5.71DD56 pKa = 5.8DD57 pKa = 6.63DD58 pKa = 7.0DD59 pKa = 6.54DD60 pKa = 5.33LVNVDD65 pKa = 4.39EE66 pKa = 5.49GDD68 pKa = 3.73VLPYY72 pKa = 10.07TFNFDD77 pKa = 3.47PFSPLNSSFFVPSHH91 pKa = 5.88HH92 pKa = 6.68HH93 pKa = 5.05HH94 pKa = 7.14HH95 pKa = 6.38NPQVNSNSCSCCSCEE110 pKa = 4.51SGSEE114 pKa = 4.19SEE116 pKa = 5.09SEE118 pKa = 4.07SDD120 pKa = 4.19CPDD123 pKa = 3.01STVYY127 pKa = 10.42PDD129 pKa = 3.62CLYY132 pKa = 11.21GHH134 pKa = 6.27EE135 pKa = 4.41EE136 pKa = 4.94EE137 pKa = 4.42YY138 pKa = 11.31DD139 pKa = 3.45QMDD142 pKa = 5.33FITDD146 pKa = 3.97LFDD149 pKa = 3.62SRR151 pKa = 11.84GEE153 pKa = 3.93IEE155 pKa = 4.9HH156 pKa = 6.89VSGTDD161 pKa = 3.06EE162 pKa = 4.94GISVSEE168 pKa = 5.32AINDD172 pKa = 3.73HH173 pKa = 7.35DD174 pKa = 5.73DD175 pKa = 4.32DD176 pKa = 4.46FVSSNIEE183 pKa = 3.72EE184 pKa = 4.52LGLGFEE190 pKa = 4.67GGGLRR195 pKa = 11.84VVGIEE200 pKa = 4.15SEE202 pKa = 4.33SDD204 pKa = 3.22SGTDD208 pKa = 3.79EE209 pKa = 4.64PGQVNDD215 pKa = 4.03QNDD218 pKa = 3.42EE219 pKa = 4.39RR220 pKa = 11.84IQITDD225 pKa = 3.33MNHH228 pKa = 3.45EE229 pKa = 4.76HH230 pKa = 6.75FWDD233 pKa = 4.55CPTFDD238 pKa = 3.57TARR241 pKa = 11.84VDD243 pKa = 4.47DD244 pKa = 4.58RR245 pKa = 11.84DD246 pKa = 3.95GLSSVIDD253 pKa = 4.33RR254 pKa = 11.84IEE256 pKa = 4.97DD257 pKa = 3.24ISVSSDD263 pKa = 2.46ISSEE267 pKa = 3.71GDD269 pKa = 3.43SAVGVLEE276 pKa = 3.99WEE278 pKa = 4.0ILLAVNNLEE287 pKa = 3.95RR288 pKa = 11.84DD289 pKa = 3.73LEE291 pKa = 4.37FEE293 pKa = 4.52TNGDD297 pKa = 3.38EE298 pKa = 4.45GFVYY302 pKa = 9.61TAEE305 pKa = 4.25FDD307 pKa = 3.69TLIGQFVEE315 pKa = 4.62TVRR318 pKa = 11.84ALRR321 pKa = 11.84GSPPAAKK328 pKa = 10.14SIIDD332 pKa = 3.69NLPSVVLKK340 pKa = 10.04TNNSKK345 pKa = 11.09DD346 pKa = 3.55GDD348 pKa = 4.1HH349 pKa = 7.48DD350 pKa = 3.91PVHH353 pKa = 6.33NQDD356 pKa = 3.84HH357 pKa = 6.73DD358 pKa = 4.05HH359 pKa = 6.79VICAVCKK366 pKa = 10.68DD367 pKa = 4.23EE368 pKa = 5.75ISMEE372 pKa = 4.2EE373 pKa = 4.56KK374 pKa = 9.6VTQLPCRR381 pKa = 11.84HH382 pKa = 6.75HH383 pKa = 5.75YY384 pKa = 10.7HH385 pKa = 7.05GDD387 pKa = 3.92CIVPWLSIRR396 pKa = 11.84NTCPVCRR403 pKa = 11.84FEE405 pKa = 6.22LPTDD409 pKa = 3.66DD410 pKa = 4.67VDD412 pKa = 4.25YY413 pKa = 10.85EE414 pKa = 4.17RR415 pKa = 11.84SKK417 pKa = 11.5NRR419 pKa = 11.84VNDD422 pKa = 3.38NEE424 pKa = 4.37LVVGYY429 pKa = 10.31GSEE432 pKa = 4.16LSPP435 pKa = 4.8

Molecular weight: 48.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A103Y954|A0A103Y954_CYNCS Protein kinase ATP binding site-containing protein OS=Cynara cardunculus var. scolymus OX=59895 GN=Ccrd_016883 PE=3 SV=1
MM1 pKa = 7.54LSLHH5 pKa = 6.65RR6 pKa = 11.84SSSVPIFKK14 pKa = 10.47LPSSSIVPLPSQSSSFHH31 pKa = 5.77RR32 pKa = 11.84HH33 pKa = 4.8PLFLFRR39 pKa = 11.84RR40 pKa = 11.84SSTRR44 pKa = 11.84IRR46 pKa = 11.84AMASNAVLAINLRR59 pKa = 11.84HH60 pKa = 5.79

Molecular weight: 6.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26434

0

26434

10709625

8

5364

405.1

45.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.461 ± 0.014

1.812 ± 0.008

5.39 ± 0.01

6.267 ± 0.017

4.278 ± 0.01

6.609 ± 0.014

2.455 ± 0.007

5.477 ± 0.011

6.074 ± 0.015

9.609 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.542 ± 0.007

4.538 ± 0.01

4.911 ± 0.017

3.57 ± 0.01

5.244 ± 0.01

8.951 ± 0.017

5.094 ± 0.009

6.587 ± 0.01

1.267 ± 0.006

2.831 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski