Salmonella phage Solent

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Sashavirus; unclassified Sashavirus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385ISY9|A0A385ISY9_9CAUD Uncharacterized protein OS=Salmonella phage Solent OX=2283281 GN=CPT_Solent_089 PE=4 SV=1
MM1 pKa = 7.83KK2 pKa = 9.23ITKK5 pKa = 9.7QEE7 pKa = 3.99LKK9 pKa = 10.99DD10 pKa = 4.7FGACQDD16 pKa = 3.33GFKK19 pKa = 10.75RR20 pKa = 11.84FVNQTNNTDD29 pKa = 3.39EE30 pKa = 4.49PVDD33 pKa = 3.9VVSLIGGCNTVSDD46 pKa = 4.7LLWLAGKK53 pKa = 7.87TLAKK57 pKa = 10.11EE58 pKa = 3.95KK59 pKa = 10.2IVRR62 pKa = 11.84FACDD66 pKa = 3.1CALLNIEE73 pKa = 5.18KK74 pKa = 10.04IRR76 pKa = 11.84PYY78 pKa = 10.44TNDD81 pKa = 2.94YY82 pKa = 10.8NLIVDD87 pKa = 4.77FLNTPTADD95 pKa = 3.68AADD98 pKa = 3.49AAYY101 pKa = 10.03AADD104 pKa = 3.52AAYY107 pKa = 10.07AADD110 pKa = 4.22AADD113 pKa = 3.59AAYY116 pKa = 10.06AAARR120 pKa = 11.84AADD123 pKa = 3.95ADD125 pKa = 3.8AAAYY129 pKa = 8.91AAYY132 pKa = 9.88AAYY135 pKa = 9.9AAYY138 pKa = 9.89AAYY141 pKa = 9.94AAARR145 pKa = 11.84AADD148 pKa = 3.95ADD150 pKa = 3.8AAAYY154 pKa = 8.91AAYY157 pKa = 9.92AAYY160 pKa = 9.99AADD163 pKa = 4.02AADD166 pKa = 3.98AADD169 pKa = 3.45AAKK172 pKa = 10.36INKK175 pKa = 9.35LLVKK179 pKa = 10.29LFSEE183 pKa = 4.76

Molecular weight: 19.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385ISD9|A0A385ISD9_9CAUD Uncharacterized protein OS=Salmonella phage Solent OX=2283281 GN=CPT_Solent_091 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 9.94PRR4 pKa = 11.84YY5 pKa = 9.61RR6 pKa = 11.84LMRR9 pKa = 11.84LSQCPNGTRR18 pKa = 11.84FYY20 pKa = 10.62FYY22 pKa = 10.67RR23 pKa = 11.84LKK25 pKa = 10.69QLLPFTLIANSFKK38 pKa = 10.28KK39 pKa = 10.09HH40 pKa = 4.64EE41 pKa = 4.86SIYY44 pKa = 10.96HH45 pKa = 6.08EE46 pKa = 3.95GHH48 pKa = 7.07DD49 pKa = 4.3FNITARR55 pKa = 11.84KK56 pKa = 8.79YY57 pKa = 8.07RR58 pKa = 11.84TSIHH62 pKa = 5.97KK63 pKa = 9.51LVWAKK68 pKa = 10.96VNN70 pKa = 3.44

Molecular weight: 8.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

17235

44

971

187.3

20.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.686 ± 0.553

1.3 ± 0.138

6.057 ± 0.234

5.918 ± 0.241

3.592 ± 0.193

7.015 ± 0.316

1.833 ± 0.165

5.698 ± 0.238

5.657 ± 0.25

7.868 ± 0.205

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.599 ± 0.154

5.199 ± 0.245

4.41 ± 0.248

4.41 ± 0.474

5.164 ± 0.27

5.762 ± 0.264

6.313 ± 0.261

7.113 ± 0.243

1.613 ± 0.133

3.795 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski