Filobacillus milosensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Filobacillus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3330 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y8IF77|A0A4Y8IF77_9BACI Peptidase S8 OS=Filobacillus milosensis OX=94137 GN=E3U55_11320 PE=4 SV=1
MM1 pKa = 7.47AFIVGCVPDD10 pKa = 4.08EE11 pKa = 4.61APVEE15 pKa = 4.21DD16 pKa = 5.34DD17 pKa = 4.66EE18 pKa = 4.94NTEE21 pKa = 4.13EE22 pKa = 5.49DD23 pKa = 4.42NSDD26 pKa = 3.65SQNDD30 pKa = 3.89SEE32 pKa = 4.97NDD34 pKa = 3.33NSSDD38 pKa = 3.52EE39 pKa = 4.61GNLSGQLEE47 pKa = 3.97IQYY50 pKa = 10.25FVGGYY55 pKa = 9.85GDD57 pKa = 3.88SWWKK61 pKa = 10.22EE62 pKa = 3.68VIGDD66 pKa = 4.2FKK68 pKa = 11.37EE69 pKa = 4.18EE70 pKa = 4.14YY71 pKa = 9.8PNVEE75 pKa = 4.49VVEE78 pKa = 4.21HH79 pKa = 6.88AGPNINEE86 pKa = 4.23EE87 pKa = 4.01MRR89 pKa = 11.84SRR91 pKa = 11.84WVSGNPPDD99 pKa = 4.1VVYY102 pKa = 10.71IDD104 pKa = 4.16GAGSSEE110 pKa = 3.98TQMVEE115 pKa = 4.61DD116 pKa = 4.27GQLMNLSEE124 pKa = 4.08WAKK127 pKa = 10.54EE128 pKa = 3.74IEE130 pKa = 4.59LEE132 pKa = 5.13DD133 pKa = 3.84GTPLLDD139 pKa = 3.89NFIAEE144 pKa = 4.22PASYY148 pKa = 10.62DD149 pKa = 3.44GEE151 pKa = 4.79LYY153 pKa = 10.87SLPLVFDD160 pKa = 4.09TWGTWYY166 pKa = 10.48DD167 pKa = 3.68KK168 pKa = 11.41AWFEE172 pKa = 4.58DD173 pKa = 3.35GGYY176 pKa = 9.8EE177 pKa = 4.25VPKK180 pKa = 10.64DD181 pKa = 3.65FEE183 pKa = 4.97SFMNTMGDD191 pKa = 3.02IKK193 pKa = 10.65ASEE196 pKa = 5.13DD197 pKa = 3.2IPPFVTTGQYY207 pKa = 10.21PYY209 pKa = 10.81YY210 pKa = 9.85FLRR213 pKa = 11.84GMLYY217 pKa = 9.92PAFSAYY223 pKa = 10.3GGDD226 pKa = 3.63EE227 pKa = 4.06LLAGVITGEE236 pKa = 3.88EE237 pKa = 4.6GAWSSEE243 pKa = 3.81EE244 pKa = 3.98VLTVMQNVQRR254 pKa = 11.84MQEE257 pKa = 3.72EE258 pKa = 5.72GYY260 pKa = 10.14IDD262 pKa = 3.83PGFGAFNHH270 pKa = 6.2TQSQMNFLLHH280 pKa = 6.53EE281 pKa = 4.24NAFIPVGFWLPNEE294 pKa = 4.27MAGDD298 pKa = 3.83TPEE301 pKa = 3.79NFQYY305 pKa = 11.21GFIPSPMNEE314 pKa = 4.28AGEE317 pKa = 4.09PMAIVPDD324 pKa = 4.2LRR326 pKa = 11.84PLAIAEE332 pKa = 4.06EE333 pKa = 4.53AEE335 pKa = 4.03NPEE338 pKa = 3.89AAKK341 pKa = 10.89AFVDD345 pKa = 4.86FVFTRR350 pKa = 11.84EE351 pKa = 3.88YY352 pKa = 11.0AKK354 pKa = 10.7LFSEE358 pKa = 4.3HH359 pKa = 6.31TGAIMNISGVDD370 pKa = 3.47LSQNEE375 pKa = 4.3NVPEE379 pKa = 3.83YY380 pKa = 10.62LIEE383 pKa = 4.59ANAMINDD390 pKa = 4.1PEE392 pKa = 4.2QVQIYY397 pKa = 8.69HH398 pKa = 6.83KK399 pKa = 8.66PHH401 pKa = 6.75PMSADD406 pKa = 3.27LEE408 pKa = 4.72TPISNSLVSLMLGNITAEE426 pKa = 4.1KK427 pKa = 9.96FIEE430 pKa = 4.3EE431 pKa = 4.1AEE433 pKa = 4.38KK434 pKa = 10.69AAEE437 pKa = 4.18EE438 pKa = 4.14YY439 pKa = 10.84RR440 pKa = 11.84NNQQ443 pKa = 2.78

Molecular weight: 49.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y8IMI9|A0A4Y8IMI9_9BACI Uncharacterized protein OS=Filobacillus milosensis OX=94137 GN=E3U55_08950 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.35QPNNRR10 pKa = 11.84KK11 pKa = 9.28RR12 pKa = 11.84SKK14 pKa = 9.57VHH16 pKa = 5.93GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 10.31SKK25 pKa = 10.6NGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.36KK37 pKa = 10.05GRR39 pKa = 11.84KK40 pKa = 8.66VLSAA44 pKa = 4.05

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3330

0

3330

921258

25

1532

276.7

31.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.11 ± 0.048

0.619 ± 0.013

5.692 ± 0.04

7.93 ± 0.053

4.608 ± 0.04

6.533 ± 0.038

2.159 ± 0.025

8.001 ± 0.039

6.796 ± 0.04

9.58 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.742 ± 0.02

4.975 ± 0.034

3.415 ± 0.024

4.04 ± 0.038

3.826 ± 0.032

5.987 ± 0.031

5.183 ± 0.025

7.028 ± 0.034

1.02 ± 0.017

3.756 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski