Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium; Sinorhizobium meliloti

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6169 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q92KC5|Q92KC5_RHIME Uncharacterized protein OS=Rhizobium meliloti (strain 1021) OX=266834 GN=SMc00940 PE=4 SV=1
MM1 pKa = 7.62LIDD4 pKa = 4.54DD5 pKa = 4.05QPEE8 pKa = 3.66ISMVLPKK15 pKa = 10.27GRR17 pKa = 11.84AMATRR22 pKa = 11.84IYY24 pKa = 8.21GTNYY28 pKa = 10.59ADD30 pKa = 4.09IIKK33 pKa = 10.28QNGYY37 pKa = 9.43IAIEE41 pKa = 4.13IYY43 pKa = 10.42AYY45 pKa = 10.66DD46 pKa = 4.56GDD48 pKa = 4.58DD49 pKa = 5.02DD50 pKa = 4.92IYY52 pKa = 11.62LNRR55 pKa = 11.84TDD57 pKa = 4.95SYY59 pKa = 11.57GGYY62 pKa = 9.83NFVDD66 pKa = 3.04AGYY69 pKa = 11.4GNDD72 pKa = 3.56LVVNSYY78 pKa = 10.96EE79 pKa = 4.22GGNDD83 pKa = 2.84IYY85 pKa = 11.24LGGGNDD91 pKa = 3.63IYY93 pKa = 11.46VADD96 pKa = 3.61IRR98 pKa = 11.84VRR100 pKa = 11.84DD101 pKa = 3.7ASSYY105 pKa = 10.3DD106 pKa = 3.14IVYY109 pKa = 10.39GGSGNDD115 pKa = 2.85RR116 pKa = 11.84FEE118 pKa = 5.18IEE120 pKa = 5.36GYY122 pKa = 10.62ASDD125 pKa = 4.55YY126 pKa = 10.89YY127 pKa = 11.36GEE129 pKa = 4.58SGNDD133 pKa = 3.1TFFSVGFNNYY143 pKa = 9.05FNGGTGTDD151 pKa = 3.56TISYY155 pKa = 9.27QFQDD159 pKa = 2.75DD160 pKa = 3.38WSAEE164 pKa = 3.97RR165 pKa = 11.84GKK167 pKa = 11.1GVTIDD172 pKa = 3.85LGYY175 pKa = 11.18NYY177 pKa = 8.51ATTGSGRR184 pKa = 11.84RR185 pKa = 11.84EE186 pKa = 3.75DD187 pKa = 4.76LISIEE192 pKa = 4.21NATGTNYY199 pKa = 10.38GHH201 pKa = 7.85DD202 pKa = 5.48DD203 pKa = 3.3ITGSAVANTLRR214 pKa = 11.84GLGGHH219 pKa = 7.18DD220 pKa = 3.55IIEE223 pKa = 4.45GLGGNDD229 pKa = 3.39VLDD232 pKa = 4.69GGSGDD237 pKa = 4.09DD238 pKa = 4.32DD239 pKa = 5.55LYY241 pKa = 11.4GGSGADD247 pKa = 3.28ILRR250 pKa = 11.84GGTGFDD256 pKa = 3.64YY257 pKa = 11.14LVGGTGTDD265 pKa = 3.0SFDD268 pKa = 3.78FNSISEE274 pKa = 4.45SAVGSRR280 pKa = 11.84RR281 pKa = 11.84DD282 pKa = 3.6VITDD286 pKa = 3.89FHH288 pKa = 8.09RR289 pKa = 11.84SEE291 pKa = 4.63FDD293 pKa = 3.7VIDD296 pKa = 4.92LSTIDD301 pKa = 6.04ADD303 pKa = 4.42TTWSGNQSFTYY314 pKa = 10.01IGGNAFSGEE323 pKa = 3.92AGEE326 pKa = 4.54LNFRR330 pKa = 11.84SGIISGDD337 pKa = 3.64VNGDD341 pKa = 3.43GYY343 pKa = 11.78ADD345 pKa = 3.46FQVRR349 pKa = 11.84VNGATSLRR357 pKa = 11.84VDD359 pKa = 4.43DD360 pKa = 5.57FFLL363 pKa = 5.77

Molecular weight: 38.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q92SH8|Q92SH8_RHIME Ribokinase OS=Rhizobium meliloti (strain 1021) OX=266834 GN=rbsK PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.06GGRR28 pKa = 11.84KK29 pKa = 9.01VLTARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6169

0

6169

1905276

14

2832

308.8

33.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.124 ± 0.035

0.861 ± 0.011

5.494 ± 0.023

6.037 ± 0.032

3.914 ± 0.022

8.538 ± 0.039

2.046 ± 0.016

5.477 ± 0.021

3.419 ± 0.023

10.021 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.519 ± 0.015

2.673 ± 0.017

4.958 ± 0.021

2.898 ± 0.016

7.169 ± 0.035

5.664 ± 0.02

5.157 ± 0.021

7.455 ± 0.024

1.299 ± 0.012

2.276 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski