Dragonfly associated cyclovirus 3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Cyclovirus

Average proteome isoelectric point is 8.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|K0A1K7|K0A1K7_9CIRC Spliced replication-associated protein OS=Dragonfly associated cyclovirus 3 OX=1234881 PE=4 SV=1
MM1 pKa = 7.9PSPRR5 pKa = 11.84DD6 pKa = 3.16LKK8 pKa = 10.82GAVRR12 pKa = 11.84LGIYY16 pKa = 8.15VLRR19 pKa = 11.84CLYY22 pKa = 10.42NGTMSARR29 pKa = 11.84QRR31 pKa = 11.84LKK33 pKa = 10.78RR34 pKa = 11.84IVFTLNNYY42 pKa = 10.06CEE44 pKa = 4.55DD45 pKa = 3.86DD46 pKa = 3.88FVRR49 pKa = 11.84IQSYY53 pKa = 9.01VEE55 pKa = 3.95IYY57 pKa = 9.68QYY59 pKa = 11.1CVVGRR64 pKa = 11.84EE65 pKa = 4.18TAPEE69 pKa = 4.04TGTPHH74 pKa = 6.13LQGFINFKK82 pKa = 9.91SKK84 pKa = 11.2RR85 pKa = 11.84EE86 pKa = 3.75FGTIKK91 pKa = 10.07TIVGDD96 pKa = 3.69RR97 pKa = 11.84AHH99 pKa = 7.14IEE101 pKa = 3.85PAKK104 pKa = 10.88GSDD107 pKa = 3.6VQNKK111 pKa = 8.23EE112 pKa = 3.92YY113 pKa = 9.25CTKK116 pKa = 10.92GGDD119 pKa = 3.41YY120 pKa = 9.68WEE122 pKa = 5.54CGTPSGPGYY131 pKa = 10.44RR132 pKa = 11.84SDD134 pKa = 4.17LADD137 pKa = 3.11VVQTVKK143 pKa = 10.32GAKK146 pKa = 9.34RR147 pKa = 11.84LRR149 pKa = 11.84EE150 pKa = 3.89VVEE153 pKa = 3.76RR154 pKa = 11.84HH155 pKa = 4.82PCEE158 pKa = 3.95FIKK161 pKa = 9.71YY162 pKa = 9.32HH163 pKa = 6.67RR164 pKa = 11.84GIEE167 pKa = 4.14KK168 pKa = 10.41LFGMLSEE175 pKa = 4.2RR176 pKa = 11.84EE177 pKa = 3.79KK178 pKa = 11.16RR179 pKa = 11.84NWKK182 pKa = 8.47TEE184 pKa = 4.04TIVYY188 pKa = 10.28YY189 pKa = 10.74GDD191 pKa = 3.83PGAGKK196 pKa = 9.66SRR198 pKa = 11.84KK199 pKa = 9.0AAEE202 pKa = 3.91VGAAAEE208 pKa = 4.23GGVYY212 pKa = 10.49YY213 pKa = 9.22KK214 pKa = 10.39TRR216 pKa = 11.84GPWWDD221 pKa = 3.66GYY223 pKa = 11.25NGEE226 pKa = 4.39TTVIVDD232 pKa = 5.39DD233 pKa = 4.36YY234 pKa = 10.71YY235 pKa = 11.83GWLAYY240 pKa = 10.53DD241 pKa = 4.01EE242 pKa = 4.4VLKK245 pKa = 9.99ITDD248 pKa = 3.59RR249 pKa = 11.84YY250 pKa = 10.33LL251 pKa = 4.8

Molecular weight:
28.65 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|K0A154|K0A154_9CIRC Replication-associated protein OS=Dragonfly associated cyclovirus 3 OX=1234881 PE=4 SV=1
MM1 pKa = 7.74AYY3 pKa = 10.44LRR5 pKa = 11.84ARR7 pKa = 11.84YY8 pKa = 6.89TNRR11 pKa = 11.84RR12 pKa = 11.84FSRR15 pKa = 11.84RR16 pKa = 11.84GLRR19 pKa = 11.84QVQRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84LIRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84PRR34 pKa = 11.84KK35 pKa = 9.96SNLSCKK41 pKa = 9.1FTTNLVLKK49 pKa = 8.44QTTGIQYY56 pKa = 11.14AHH58 pKa = 7.68IAPALEE64 pKa = 4.82DD65 pKa = 3.67FAEE68 pKa = 4.42TTNLITNFEE77 pKa = 4.39YY78 pKa = 11.07YY79 pKa = 10.45KK80 pKa = 10.06IRR82 pKa = 11.84KK83 pKa = 8.51VRR85 pKa = 11.84ITVTPRR91 pKa = 11.84NNVAYY96 pKa = 10.1QGQTVPDD103 pKa = 3.75YY104 pKa = 11.24VIAPFHH110 pKa = 6.76HH111 pKa = 6.77PVDD114 pKa = 4.46DD115 pKa = 4.33KK116 pKa = 11.64SISVDD121 pKa = 3.33SLLTLDD127 pKa = 3.23RR128 pKa = 11.84HH129 pKa = 5.37KK130 pKa = 10.66KK131 pKa = 9.38YY132 pKa = 10.59RR133 pKa = 11.84GTQRR137 pKa = 11.84GHH139 pKa = 4.75MTFKK143 pKa = 10.31PAVIGLASTSLDD155 pKa = 3.7DD156 pKa = 4.94SSGTYY161 pKa = 9.1ATMRR165 pKa = 11.84WSPKK169 pKa = 10.12ILITNNTTPQQVKK182 pKa = 9.72HH183 pKa = 5.4FCGLLAFAPQGSNAQEE199 pKa = 3.79YY200 pKa = 10.07EE201 pKa = 3.98ITLDD205 pKa = 4.2AYY207 pKa = 10.17CTFYY211 pKa = 10.57NQKK214 pKa = 9.89INKK217 pKa = 8.94LLL219 pKa = 3.84

Molecular weight:
25.39 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

770

219

300

256.7

29.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.714 ± 0.44

1.948 ± 0.229

4.935 ± 0.33

5.714 ± 1.265

3.377 ± 0.296

9.091 ± 1.823

2.078 ± 0.259

5.195 ± 0.3

6.494 ± 0.285

7.013 ± 0.692

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.169 ± 0.088

3.636 ± 0.672

4.286 ± 0.132

3.117 ± 0.868

8.571 ± 0.709

4.416 ± 0.425

7.662 ± 0.878

7.143 ± 0.773

1.688 ± 0.458

6.753 ± 0.482

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski