Sewage-associated circular DNA virus-37

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UI36|A0A0B4UI36_9VIRU Capsid protein OS=Sewage-associated circular DNA virus-37 OX=1592104 PE=4 SV=1
MM1 pKa = 7.82PFRR4 pKa = 11.84VSAKK8 pKa = 10.28SFIITYY14 pKa = 7.68PRR16 pKa = 11.84CDD18 pKa = 3.1VDD20 pKa = 5.69KK21 pKa = 11.05EE22 pKa = 4.77VILQWFQDD30 pKa = 3.18HH31 pKa = 6.86FMLLGCRR38 pKa = 11.84VARR41 pKa = 11.84EE42 pKa = 3.84LHH44 pKa = 6.72ADD46 pKa = 4.03GTPHH50 pKa = 5.84IHH52 pKa = 6.11VVFHH56 pKa = 6.94IANTFQTRR64 pKa = 11.84NARR67 pKa = 11.84YY68 pKa = 9.2FDD70 pKa = 4.1IGDD73 pKa = 3.5HH74 pKa = 6.32HH75 pKa = 7.48PNVQSTRR82 pKa = 11.84GLPASYY88 pKa = 10.4AYY90 pKa = 9.75VAKK93 pKa = 10.81DD94 pKa = 2.94GDD96 pKa = 4.34YY97 pKa = 10.68IDD99 pKa = 5.7FGTIPATRR107 pKa = 11.84PHH109 pKa = 6.75GSWADD114 pKa = 3.28IANATTKK121 pKa = 10.72EE122 pKa = 3.73EE123 pKa = 3.79ALALVKK129 pKa = 9.21EE130 pKa = 4.51TSPRR134 pKa = 11.84DD135 pKa = 3.3YY136 pKa = 11.26VLNYY140 pKa = 10.65DD141 pKa = 4.71KK142 pKa = 10.64IISFCEE148 pKa = 3.51SHH150 pKa = 6.3FANRR154 pKa = 11.84TEE156 pKa = 4.2AYY158 pKa = 10.2VPIYY162 pKa = 10.71QNFNGLPQQLTTWCDD177 pKa = 3.01QRR179 pKa = 11.84LEE181 pKa = 4.02VSEE184 pKa = 4.26RR185 pKa = 11.84HH186 pKa = 5.76SPPPAAGLPPQPPTEE201 pKa = 4.1WGEE204 pKa = 3.78LLYY207 pKa = 10.34IVTNQSLGHH216 pKa = 5.97HH217 pKa = 6.45MYY219 pKa = 10.45FNAMFDD225 pKa = 3.41LGEE228 pKa = 4.2WDD230 pKa = 5.63DD231 pKa = 3.91GAQYY235 pKa = 11.16AIFDD239 pKa = 4.36DD240 pKa = 3.71WADD243 pKa = 2.99WSKK246 pKa = 11.03FYY248 pKa = 10.66AYY250 pKa = 9.67KK251 pKa = 10.48QFLGAQRR258 pKa = 11.84QFTITDD264 pKa = 3.49KK265 pKa = 10.76YY266 pKa = 9.4RR267 pKa = 11.84RR268 pKa = 11.84KK269 pKa = 9.33RR270 pKa = 11.84TVRR273 pKa = 11.84WGKK276 pKa = 8.56PAIVISNLRR285 pKa = 11.84PAWEE289 pKa = 4.44DD290 pKa = 3.5EE291 pKa = 3.83DD292 pKa = 4.61WITVNCFQCHH302 pKa = 5.39LLGALFF308 pKa = 4.26

Molecular weight: 35.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UI36|A0A0B4UI36_9VIRU Capsid protein OS=Sewage-associated circular DNA virus-37 OX=1592104 PE=4 SV=1
MM1 pKa = 7.7PHH3 pKa = 6.6RR4 pKa = 11.84NWRR7 pKa = 11.84KK8 pKa = 7.49RR9 pKa = 11.84THH11 pKa = 6.96DD12 pKa = 3.47ALHH15 pKa = 6.3RR16 pKa = 11.84VGSHH20 pKa = 4.85VLRR23 pKa = 11.84NGKK26 pKa = 7.9TYY28 pKa = 11.0GDD30 pKa = 3.71VYY32 pKa = 11.1SAYY35 pKa = 9.85KK36 pKa = 9.95KK37 pKa = 10.77LKK39 pKa = 7.52THH41 pKa = 6.75HH42 pKa = 6.46SSEE45 pKa = 4.38RR46 pKa = 11.84SIPMAPKK53 pKa = 10.36DD54 pKa = 3.45ALTNQFNAQTIFRR67 pKa = 11.84RR68 pKa = 11.84KK69 pKa = 9.55KK70 pKa = 7.64NRR72 pKa = 11.84RR73 pKa = 11.84WRR75 pKa = 11.84KK76 pKa = 8.46QLSFAQKK83 pKa = 10.06VLRR86 pKa = 11.84ANQIFSSEE94 pKa = 3.67KK95 pKa = 8.82TYY97 pKa = 11.42QLMRR101 pKa = 11.84TWASAAAAGVQGFNGISLYY120 pKa = 11.4ANGSGDD126 pKa = 4.17ADD128 pKa = 3.63VNDD131 pKa = 3.8IFQAYY136 pKa = 10.31DD137 pKa = 3.11NVTSRR142 pKa = 11.84EE143 pKa = 3.35WSINMKK149 pKa = 9.81SAHH152 pKa = 6.19VDD154 pKa = 3.52YY155 pKa = 11.1LCTNVGSTTLVLKK168 pKa = 10.71VYY170 pKa = 9.65TIVPKK175 pKa = 10.49KK176 pKa = 9.86DD177 pKa = 3.18APVAAGNNLGNAWIASLDD195 pKa = 3.61MSVAMPGSTVGLDD208 pKa = 3.15QIVANPVGTPARR220 pKa = 11.84PGATPFDD227 pKa = 3.77SSVFCSQYY235 pKa = 10.6TVIKK239 pKa = 9.05CVEE242 pKa = 4.41YY243 pKa = 10.24ILPPGQVASFNDD255 pKa = 2.89SMMRR259 pKa = 11.84RR260 pKa = 11.84GKK262 pKa = 10.73LDD264 pKa = 3.26TAEE267 pKa = 4.28FNVGVATTKK276 pKa = 10.44TNWAVKK282 pKa = 9.54GWTKK286 pKa = 10.67SHH288 pKa = 6.58LFVHH292 pKa = 6.6HH293 pKa = 7.09GIATSGAVSSLATYY307 pKa = 10.33YY308 pKa = 9.68PASSLGVIANKK319 pKa = 9.21TYY321 pKa = 10.88KK322 pKa = 9.74FTVNEE327 pKa = 3.71QRR329 pKa = 11.84NQSEE333 pKa = 4.18IQVASTVTT341 pKa = 3.13

Molecular weight: 37.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

649

308

341

324.5

36.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.553 ± 0.525

1.387 ± 0.374

5.547 ± 1.064

3.236 ± 0.873

4.777 ± 0.928

5.855 ± 0.44

3.544 ± 0.451

4.931 ± 0.609

5.085 ± 1.009

6.163 ± 0.437

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.849 ± 0.367

5.239 ± 0.895

4.931 ± 0.826

4.468 ± 0.268

5.547 ± 0.198

6.78 ± 1.706

7.088 ± 0.613

7.088 ± 1.262

2.619 ± 0.418

4.314 ± 0.371
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski